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Preparation method of high-purity anthocyanins by extracting from roselle

An anthocyanin and roselle technology, applied in sustainable manufacturing/processing, chemical industry, climate sustainability, etc., can solve problems such as large solvent consumption and large solvent loss, and achieve shortened extraction time, low cost, and continuous The effect of large-scale industrial production

Inactive Publication Date: 2016-08-17
JIANGXI HAIFU BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the use of organic solvents for extraction, although the purity of anthocyanins in the extract will be higher, the amount of solvent used is relatively large, and the solvent loss is relatively large

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1) Pretreatment of raw materials ： Properly crush 1000Kg of raw materials with a crusher, and most of them pass through a 20-40 mesh sieve as the standard.

[0034] 2) Extraction: use water as solvent, adopt the continuous countercurrent extraction method, first soak in 8-10T cold water to remove impurities, and then use 10T 45-55℃ hot water for continuous countercurrent extraction; separate the cold water and hot water extracts and collect them separately. 200 mesh filter bag to obtain filtrate.

[0035] 3) Enzymolysis: the hot water extract is enzymatically hydrolyzed with cellulase, pectinase, and protease compound enzyme. The compound enzyme is an imported liquid enzyme, and the compound ratio of cellulose: pectinase: protease is 2 to 3 :1~2:0.5~1, the amount of compound enzyme added is 0.01~0.02% of the extract volume, the enzymolysis temperature is controlled at 30~40°C, the enzymolysis time is controlled for 2 hours, and the enzymolysis pH is controlled at 2~3. ...

Embodiment 2

[0043] 1) Pretreatment of raw materials: Properly crush 1000Kg of raw materials with a crusher, and most of them pass through a 20-40 mesh sieve as the standard.

[0044] 2) Extraction: use water as solvent, adopt the continuous countercurrent extraction method, first soak in 8-10T cold water to remove impurities, and then use 10T 45-55℃ hot water for continuous countercurrent extraction; separate the cold water and hot water extracts and collect them separately. 200 mesh filter bag to obtain filtrate.

[0045]3) Enzymolysis: use cellulase, pectinase, and protease compound enzymes to carry out enzymolysis of the hot water extract. The compound enzymes are imported liquid enzymes, and the compound ratio of cellulose: pectinase: protease is 2 to 3:1 ~2:0.5~1, the amount of compound enzyme added is 0.03~0.05% of the volume of the extract, the enzymolysis temperature is controlled at 40~45°C, the enzymolysis time is controlled for 2 hours, and the enzymolysis pH is controlled at 2...

Embodiment 3

[0053] 1) Pretreatment of raw materials: Properly crush 1000Kg of raw materials with a crusher, and most of them pass through a 20-40 mesh sieve as the standard.

[0054] 2) Extraction: use water as solvent, adopt the continuous countercurrent extraction method, first soak in 8-10T cold water to remove impurities, and then use 10T 45-55℃ hot water for continuous countercurrent extraction; separate the cold water and hot water extracts and collect them separately. 200 mesh filter bag to obtain filtrate.

[0055] 3) Enzymolysis: use cellulase, pectinase, and protease compound enzymes to carry out enzymolysis of the hot water extract. The compound enzymes are imported liquid enzymes, and the compound ratio of cellulose: pectinase: protease is 2 to 3:1 ~2:0.5~1, the amount of compound enzyme added is 0.02~0.04% of the volume of the extract, the enzymolysis temperature is controlled at 40~45°C, the enzymolysis time is controlled for 2 hours, and the enzymolysis pH is controlled at ...

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PUM

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Abstract

The invention discloses a preparation method of high-purity anthocyanins by extracting from roselle, and relates to the extraction method of anthocyanins. The preparation method successively comprises the following specific steps: raw material pretreatment, extraction, enzymatic hydrolysis, centrifugalization, ultrafiltration, nanofiltration, concentration, microwave drying and crushing. The preparation method has the beneficial effects that the purity of an anthocyanin extract of the roselle can be improved from 2 to 3% to 30% or above, the energy can be saved, the process is simple, the cost is low, and the mass production can be facilitated.

Description

technical field [0001] The invention relates to a method for extracting anthocyanins, in particular to a method for extracting anthocyanins from roselles. Background technique [0002] Roselle, also known as roselle, roselle, mountain eggplant, etc. Originally produced in West Africa and India, it is currently cultivated in Guangdong, Guangxi, Fujian, Yunnan, Taiwan and other places in my country. Roselles are generally picked in mid-to-late November, and the calyx is often used as the extraction part. Roselle is rich in protein, pectin, organic acids, VC, amino acids, mineral elements, polyphenols and anthocyanins. The main active ingredients are anthocyanins and polyphenols. Medical research has proved that roselle anthocyanins mainly have pharmacological effects such as protecting eyesight, anti-oxidation, anti-tumor and antibacterial. [0003] At present, the extraction methods of roselle anthocyanins mainly include solvent extraction, continuous countercurrent extrac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62
CPCC07D311/62Y02P20/10
Inventor 陈钱龙伟岸黄华学
Owner JIANGXI HAIFU BIOENG
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