Continuous chromatographic separation and purification system for separating sugar in inulin

A continuous chromatography, separation and purification technology, applied in the field of liquid separation and purification, can solve the problems of low resin utilization efficiency, low resin utilization rate, large discharge of acid-base waste liquid, etc., achieve good separation and purification effect, improve production efficiency, improve Effect of Utilization and Process Yield

Active Publication Date: 2015-12-30
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
View PDF5 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] When the fixed bed intermittently separates fructo-oligosaccharides and polyfructosaccharides, only a small amount of resin in the mass transfer area is working in the entire bed, and most of the resin is in an inactive idle and waiting state, which has the disadvantage of low utilization rate of the resin; at the same time A large amount of material or regenerant needs to pass through or soak the deactivated resin, causing pollution to the r

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Continuous chromatographic separation and purification system for separating sugar in inulin
  • Continuous chromatographic separation and purification system for separating sugar in inulin
  • Continuous chromatographic separation and purification system for separating sugar in inulin

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0038] Example 1:

[0039] A. Prepare 10L of 100g / L inulin solution, connect the instrument according to the operating rules, connect 75L pure water in zone 1 and 5L pure water in zones 2 and 3;

[0040] B. Set the experimental parameters of the panel and the pump flow, and press the "turntable to find the origin" button at the same time, clear the time, and set the feed flow rate of the peristaltic pump to 5ml / min;

[0041] Location

[0042] C. Turn on the pump and press the "Turntable start" button, then ensure that the instrument does not leak and runs normally;

[0043] D. Take samples after running the experiment for 24 hours, and analyze the sample points on the TLC plate. The sampling results of zone 1 and zone 3 are shown respectively image 3 The second and third TLC results.

Example Embodiment

[0044] Example 2:

[0045] A. Prepare 10L of 200g / L inulin solution, connect the instrument according to the flow chart, connect 75L pure water in zone 1 and 5L pure water in zone 2 and 3;

[0046] B. Set the experimental parameters of the panel and pump flow, and press the "turntable to find origin" button at the same time, clear the time, and set the feed flow rate of the peristaltic pump to 4ml / min

[0047] Location

[0048] C. Turn on the pump and press the "Turntable start" button, then ensure that the instrument does not leak and runs normally;

[0049] D. Take samples after running the experiment for 24 hours, and analyze the sample points on the TLC plate. The sampling results of zone 1 and zone 3 are shown respectively image 3 The 4th and 5th TLC results.

Example Embodiment

[0050] Example 3:

[0051] A. Prepare 10L of 200g / L inulin solution, connect the instrument according to the flow chart, connect 75L pure water in zone 1 and 5L pure water in zone 2 and 3;

[0052] B. Set the experimental parameters of the panel and the pump flow, and press the "turntable to find the origin" button at the same time, clear the time, and set the feed flow rate of the peristaltic pump to 5ml / min;

[0053] Location

[0054] C. Turn on the pump and press the "Turntable start" button, then ensure that the instrument does not leak and runs normally;

[0055] D. Take samples after running the experiment for 24 hours, and analyze the sample points on the TLC plate. The sampling results of zone 1 and zone 3 are shown respectively image 3 The 7th and 6th TLC results.

[0056] See image 3 For each strip from left to right: 1 lane is inulin; 2 lanes are sampling in area 1 of Example 1, lane 3 is sampling in area 3 of Example 1, and 4 lanes are sampling in area 1 of Example 2....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Login to view more

Abstract

The invention provides a continuous chromatographic separation and purification system for separating sugar in inulin. A plurality of separation units which are connected together in series are driven by a turntable to rotate, and when the turntable rotates a circle, under the action of cation exchange resin filled in the separation units, fructo-oligosaccharide flows out of an elution area, polyfructose flows out of a separation area, and recycled water flows out of a concentration area. In such a manner, an inulin solution is separated and purified, so that links of exchange, washing, recycling, leaching and the like in an original fixed bed are integrated in the same system to achieve a better separation and purification effect.

Description

technical field [0001] The invention relates to the technical field of liquid separation and purification, in particular to a continuous chromatographic separation and purification system for separating sugar in inulin. Background technique [0002] Inulin is a fructan mixture in which fructose molecules are connected by β-(2-1) glycosidic bonds and end with a glucose molecule. Currently, inulin is mainly derived from Jerusalem artichoke and chicory. The degree of polymerization of fructose units in natural inulin is usually 2-60, with an average degree of polymerization of about 10. [0003] Fructans with a polymerization degree of 2-9 in inulin are commonly called fructo-oligosaccharides, and the content of fructo-oligosaccharides in inulin is generally 5-35%. Fructose-oligosaccharide is a natural active substance, a new type of sweetener with health functions such as regulating intestinal flora, multiplying bifidobacteria, maintaining the balance of intestinal flora, pr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): B01D15/04
Inventor 李莉莉秦松张银焦绪栋崔玉琳
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap