The invention provides a preparation method of a notopterygium root spray of nine ingredients, relating to a preparation method of a traditional Chinese medicine spray. The notopterygium root spray of nine ingredients is composed of notopterygium root, divaricate saposhnikovia root, Chinese atractylodes, asarum herb, chuanxiong rhizome, angelia, baical skullcap root, licorice and rehmannia root. The preparation method comprises the following steps of: extracting volatile oils from the notopterygium root, the divaricate saposhnikovia root, the Chinese atractylodes, the asarum herb, the chuanxiong rhizome and the angelica; performing ultrasonic extraction on the dregs after extraction, the baical skullcap root, the licorice and the rehmannia root, filtering after extraction; concentrating the obtained filtrate, adding ethanol, standing and filtering; and concentrating the filtrate, recovering the ethanol, adding the volatile oils, a surfactant, an essence and a preservative to the concentrated solution, mixing evenly, supplementing distilled water, filtering, subpackaging and sterilizing. The preparation method provided by the invention has the characteristics of short extraction time, high yield, no need of heating, and the like, saves the production time, improves the extraction rate, avoids degradation of thermal instable ingredients, is favorable to full utilization of medicinal materials, and saves energy and cost.