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Flash extraction method of achyranthes root polysaccharide

A technology of flash extraction and flash extractor, which is applied in the field of flash extraction of Achyranthes bidentata polysaccharides, can solve the problems of long extraction time, destruction of polysaccharide structure and biological activity, and low extraction rate, so as to achieve short extraction time and maintain Structural integrity and biological activity, the effect of high extraction rate

Inactive Publication Date: 2013-08-21
NORTH CHINA UNIV OF WATER RESOURCES & ELECTRIC POWER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for extracting and purifying Achyranthes bidentata polysaccharides, to overcome the deficiencies in the prior art, mainly to solve the problems of long extraction time, low extraction rate, polysaccharide structure and biological factors in the existing extraction process. The problem of large activity damage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The method for extracting Achyranthes bidentata polysaccharides from Achyranthes bidentata in this embodiment is realized in the following steps:

[0020] (1) Flash extraction: Weigh 20g of Achyranthes bidentata powder, add 200mL of pure water, mix well, transfer to a flash extractor container, and extract with a flash extractor. The residue was extracted again in the same way, with a single extraction time of 2 minutes and an extraction temperature of 50°C, and the two extractions were combined.

[0021] (2) Centrifugation: centrifuge the extract obtained in step (1) at 4° C. and 4000 rpm for 8 minutes.

[0022] (3) Concentration: Concentrate the supernatant obtained in step (2) with a flash concentrator.

[0023] (4) Alcohol precipitation: Add 3 times the volume of absolute ethanol to the concentrated solution obtained in step (3) to carry out alcohol precipitation, stand at 4°C for 24 hours, and then centrifuge to obtain the precipitate.

[0024] (5) Impurity remov...

Embodiment 2

[0027] The method for extracting Achyranthes bidentata polysaccharides from Achyranthes bidentata in this embodiment is realized in the following steps:

[0028] (1) Flash extraction: Weigh 50g of Achyranthes bidentata powder, add 750mL of pure water, mix well, transfer to a flash extractor container, and extract with a flash extractor. The residue was extracted again in the same way, with a single extraction time of 3 minutes and an extraction temperature of 60°C, and the two extractions were combined.

[0029] (2) Centrifugation: centrifuge the extract obtained in step (1) at 4° C. and 5000 rpm for 10 minutes.

[0030] (3) Concentration: Concentrate the supernatant obtained in step (2) with a flash concentrator.

[0031] (4) Alcohol precipitation: Add 4 times the volume of absolute ethanol to the concentrated solution obtained in step (3) to carry out alcohol precipitation, stand still at 4° C. for 24 hours, and then centrifuge to obtain the precipitate.

[0032] (5) Impur...

Embodiment 3

[0035] (1) Flash extraction: Weigh 100g of Achyranthes bidentata powder, add 1500mL of pure water, mix well, transfer to a flash extractor container, and extract with a flash extractor. The residue was extracted again in the same way, with a single extraction time of 3 minutes and an extraction temperature of 50°C, and the two extractions were combined.

[0036] (2) Centrifugation: centrifuge the extract obtained in step (1) at 4° C. and 5000 rpm for 8 minutes.

[0037] (3) Concentration: Concentrate the supernatant obtained in step (2) with a flash concentrator.

[0038] (4) Alcohol precipitation: Add 4 times the volume of absolute ethanol to the concentrated solution obtained in step (3) to carry out alcohol precipitation, stand still at 4° C. for 24 hours, and then centrifuge to obtain the precipitate.

[0039] (5) Impurity removal: dissolve the precipitate obtained in step (4) with pure water 30 times the weight of the precipitate, filter and concentrate the obtained solu...

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Abstract

The invention discloses a flash extraction method of achyranthes root polysaccharide. The method is characterized by comprising the following steps: adding water to achyranthes root powder for flash extraction, and carrying out centrifugation and flash concentration on the extracting liquor; adding absolute ethyl alcohol to the concentrated liquor for natural alcohol precipitation, and standing and centrifuging to obtain a precipitate; adequately dissolving the precipitate by adding water, and after filtering, concentrating by a flash concentrator; and carrying out vacuum drying on the concentrated liquor to obtain an achyranthes root polysaccharide product. According to the method, the extraction time can be shortened, the use level of extraction liquor can be reduced, and the extraction rate can be improved. The method provided by the invention is simple and convenient for extraction process and low in equipment operation and maintenance cost. The structural integrity and the biological activity of polysaccharide are beneficially maintained.

Description

technical field [0001] The invention relates to a preparation method of plant polysaccharides, in particular to a flash extraction method of Achyranthes japonicus polysaccharides. Background technique [0002] Huai Achyranthes bidentata is one of the "Four Huai Medicines" of the famous authentic medicinal materials, which is produced in Jiaozuo City, Henan Province. It is a commonly used and precious health-care medicinal material. Because of its magical medicinal effect, unique nourishing effect and long history, it has been regarded as a treasure of traditional Chinese medicine by ancient Chinese medicine classics in my country, and enjoys the reputation of tribute in history. Jiaozuo City, Henan Province, with its unique geographical environment, traditional planting technology and processing technology, has created the excellent quality of Achyranthes bidentata as an authentic medicinal material, which is different from other origins of Achyranthes bidentata, and has obt...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 宋小勇
Owner NORTH CHINA UNIV OF WATER RESOURCES & ELECTRIC POWER
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