Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for purifying cheliensisin A

A technology of carnation and fragrant leaves, which is applied in the field of supercritical CO2 extraction and high-speed countercurrent chromatography to purify Jinghong gonacarin, which can solve the problems of low extraction efficiency and yield, environmental pollution, etc., and achieve a large amount of preparation , short extraction time and high efficiency

Inactive Publication Date: 2012-01-25
苏州宝泽堂医药科技有限公司
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this traditional process has relatively low extraction efficiency and yield, which seriously pollutes the environment.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Take Jinghonggena fragrant leaf medicinal material, crush 60 mesh, take 500g of raw material and add supercritical CO 2 In the extraction kettle, turn on the equipment and adjust the parameters: the extraction temperature is 50°C, the extraction pressure is 25MPa, the temperature of the separation kettle I is 55°C, and the pressure is 10MPa, the temperature of the separation kettle II is 45°C, and the pressure is 5MPa. After reaching the above parameters, extract at a flow rate of 20L / h for 4h, and analyze the extract. Take n-hexane, ethyl acetate, methanol, and water, and mix them in a ratio of 3:3:5:4. The following phase is the stationary phase, and the upper phase is the mobile phase. Turn on the high-speed countercurrent chromatograph, and inject the dissolved extract of ethyl acetate into the Chromatograph, adjust rotating speed 900r / min, flow velocity 2ml / min, separate to obtain jinghongfen, collect jinghong, and dry it under reduced pressure to obtain jinghong, ...

Embodiment 2

[0017] Take Jinghong Gona fragrant leaf medicinal material, crush 60 mesh, take 500g raw material and add CO 2 In the extraction kettle, turn on the equipment and adjust the parameters: the extraction temperature is 50°C, the extraction pressure is 20MPa, the temperature of the separation kettle I is 50°C, and the pressure is 8MPa, the temperature of the separation kettle II is 45°C, and the pressure is 6MPa. After reaching the above parameters, extract at a flow rate of 30L / h for 2h and collect the extract. Take n-hexane, ethyl acetate, methanol, and water, and mix them in a ratio of 3:5:4:4. The following phase is the stationary phase, and the upper phase is the mobile phase. Turn on the high-speed countercurrent chromatograph, and inject the dissolved extract of ethyl acetate into the Chromatograph, adjust rotating speed 850r / min, flow velocity 2ml / min, separate jinghongcona, collect jinghongzheng fraction, dry under reduced pressure to get jinghongcona 671mg, high performa...

Embodiment 3

[0019] Take Jinghong Gona fragrant leaf medicinal material, crush 80 mesh, take 1kg of raw material and add CO 2 In the extraction kettle, turn on the equipment and adjust the parameters: the extraction temperature is 55°C, the extraction pressure is 22MPa, the temperature of the separation kettle I is 50°C, and the pressure is 8MPa, the temperature of the separation kettle II is 45°C, and the pressure is 6MPa. After reaching the above parameters, extract at a flow rate of 30L / h for 2h and collect the extract. Take n-hexane, ethyl acetate, methanol, and water, and mix them in a ratio of 3:4:5:4. The following phase is the stationary phase, and the upper phase is the mobile phase. Turn on the high-speed countercurrent chromatograph, and inject the ethyl acetate-dissolved extract into the chromatogram. Apparatus, adjust the rotating speed 850r / min, flow rate 2ml / min, separate jinghongcona, collect jinghongcona fraction, dry under reduced pressure to get jinghongcona 1.31g, high ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for purifying cheliensisin A. The method is characterized by comprising the following steps: taking leaves of goniothalamus cheliensis as raw materials, grinding the leaves to 60-80 meshes, adding the ground leaves to an extraction kettle, extracting the leaves by adopting supercritical CO2, collecting the extracts, adopting a high-speed counter-current chromatography for separation and drying stream under reduced pressure by taking n-hexane-ethyl acetate-methanol-water as a solvent system, thus obtaining cheliensisin A. The method has the advantages of high efficiency, environment friendliness and the like.

Description

technical field [0001] The present invention relates to a kind of method of purifying Jinghong cornagarin, in particular to a method of using supercritical CO 2 Methods of extraction and high-speed countercurrent chromatography purification of Jinghong genarin. Background technique [0002] Jinghong Cheliensisin A, chemical name 6(7,8-epoxy-styryl)-5-acetoxy-5,6-dihydro-2-pyrone, molecular weight 274, White crystal, melting point 152-153°C. [0003] Jinghong Gonagenin A is extracted from the leaves of Jinghong Gonagarin, an anemone plant, and has a strong anti-tumor effect. Both the literature "Jinghong Gnagarin Induces Human Promyelocytic Leukemia Cell Apoptosis and Its Signal Transduction Pathway" and the patent "Jinghong Gnagarin A and Its Preparation and Application" have reported Significant anti-tumor effects of nalcohol. [0004] The existing method for purifying Jinghong Gonagenin is as follows: Jinghong Gonaxiang leaves or stems are extracted by cold immersion i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07D407/04
CPCY02P20/54Y02P20/582
Inventor 李法庆刘东锋
Owner 苏州宝泽堂医药科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com