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Method for extracting almond protein by microwave

A technology of almond protein and almonds, which is applied to the protein composition of vegetable seeds, protein food ingredients, food science, etc., can solve the problems of low extraction rate, high energy consumption, and long extraction time, and achieve high extraction rate and long extraction time. Short, the effect of maintaining functional properties and nutritional value

Inactive Publication Date: 2011-08-17
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The above-mentioned methods have the following defects: some have high energy consumption, some have long extraction time, and some have low extraction rate

Method used

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  • Method for extracting almond protein by microwave
  • Method for extracting almond protein by microwave

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for microwave extraction of almond protein, comprising the steps of:

[0021] (1) Crush the degreased almond residue through a 40-mesh sieve, accurately weigh a certain amount of almond residue powder, and dissolve it in distilled water at room temperature; the mass ratio of the almond residue powder to water is 1:30;

[0022] (2) Under the conditions of microwave power 350W, frequency 915MHz, temperature 55℃, pH=11, microwave extraction for 25min;

[0023] (3) Under the condition of 4000 rpm, centrifuge for 5 minutes, remove the sediment in the lower layer after centrifugation; let the upper liquid stand for 10 minutes, remove the oil layer on the liquid surface, and obtain substance A; the adjustment of the pH value is 1.5mol / L NaOH aqueous solution;

[0024] (4) Adjust substance A with 2% HCl aqueous solution, adjust its pH=4.5, and centrifuge for 3 minutes at 4000 rpm; remove the upper layer solution, take the precipitate, and obtain substance B;

[0025]...

Embodiment 2

[0027] A method for microwave extraction of almond protein, comprising the steps of:

[0028] (1) Crush the degreased almond residue through a 40-mesh sieve, accurately weigh a certain amount of almond residue powder, and dissolve it in distilled water at room temperature; the mass ratio of the almond residue powder to water is 1:35;

[0029] (2) Under the conditions of microwave power 250W, frequency 915 MHz, temperature 50°C, pH=11.5, microwave extraction for 15 minutes;

[0030] (3) Under the condition of 3500 rpm, centrifuge for 6 minutes, remove the sediment in the lower layer after centrifugation; let the upper liquid stand for 8 minutes, remove the oil layer on the liquid surface, and obtain substance A; the adjustment of the pH value is 0.5mol / L of NaOH aqueous solution;

[0031] (4) Adjust substance A with 3% HCl aqueous solution to make it pH = 4.5, and centrifuge it for 4 minutes at 3500 rpm; remove the upper layer solution, take the precipitate, and obtain substa...

Embodiment 3

[0034] A method for microwave extraction of almond protein, comprising the steps of:

[0035] (1) Crush the degreased almond residue through a 40-mesh sieve, accurately weigh a certain amount of almond residue powder, and dissolve it in distilled water at room temperature; the mass ratio of the almond residue powder to water is 1:40;

[0036] (2) Under the conditions of microwave power 150W, frequency 915MHz, temperature 45°C, pH=10.5, microwave extraction for 20 minutes;

[0037] (3) Under the condition of 4500 rpm, centrifuge for 4 minutes, remove the sediment in the lower layer after centrifugation; let the upper liquid stand for 5 minutes, remove the oil layer on the liquid surface, and obtain substance A; the pH value is adjusted using 1mol / L NaOH aqueous solution;

[0038] (4) Adjust substance A with 2% HCl aqueous solution to make it pH = 4.5, and centrifuge it for 2 minutes at 4500 rpm; remove the upper layer solution, take the precipitate, and obtain substance B;

...

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Abstract

The invention relates to a method for extracting almond protein by microwave. In the method, with almond residuals subjected to oil removal and degrease removal as a raw material, the almond protein is prepared through the steps of raw material sieving, water dissolution, microwave extraction, centrifugal separation, pH value regulation with HCl, vacuum freeze-drying and the like. The invention has the characteristics and beneficial effects: a microwave solution promotion technology is utilized, thus the advantages of energy conservation, environmental friendliness, short extraction time, high extraction rate and the like are achieved; compared with an extraction technology in an ultrasonic environment, the time required by the microwave solution promotion technology is shortened by 20-60min, and the extraction rate of the almond protein under the optimal extraction condition is improved by 16.7%; compared with the common extraction technology, the time required by the microwave solution promotion technology is shortened by 95-105min, and the extraction rate of the almond protein under the optimal extraction condition is improved by 30.7%; meanwhile, when the almond protein is dried, the vacuum freeze-drying technology is utilized, and thus the prepared almond protein product can better maintain the original functional characteristics and nutritional value.

Description

[0001] Technical field [0002] The invention involves a method of extraction of almond protein, especially a method of microwave extraction almond protein. Background technique [0003] The protein in almonds is a high -quality plant protein with superior functional characteristics and high nutritional value.Not only can it be used as a pharmaceutical raw material, health products, nutrition products, functional foods, food enhancers, infant food and elderly food additives, etc., but also can be used as animal feed additives, antioxidants, preservatives, etc.There will be no environmental pollution.At present, there are many research on using almonds to extract plant protein with almonds. For example, patented CN1663407 (200510041901.6) disclosed a process to make almond oil and almond protein powder; CN1116069 (95103365.4) disclosed a acidic hybrid juice (pulp flesh flesh (pulp flesh flesh)) Almond protein beverage; patented CN1385082 (01116049.7) revealed a method of preparing...

Claims

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Application Information

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IPC IPC(8): A23J1/14
Inventor 包晓霞牛天水李凯梁懂懂王茂森张继梁俊玉王云普姚健马君义
Owner NORTHWEST NORMAL UNIVERSITY
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