Method for simply and rapidly extracting total RNA from switchgrass tissue
A switchgrass, rapid technology, applied in the direction of recombinant DNA technology, DNA preparation, etc., can solve the problems of long time consumption, affecting the extraction efficiency of switchgrass, difficult to grind thoroughly, etc., and achieve the effect of low cost, large amount of RNA, and short extraction time
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[0024] Example 1
[0025] A. Preparation of experimental drugs: RNA extract with a volume fraction of 0.1‰ DEPC water and a volume fraction of 70% ethanol (prepared with 0.1‰ DEPC water).
[0026] B. Treatment of experimental supplies: After the utensils were soaked in DEPC water with a volume fraction of 0.1‰ overnight, sterilized at 120°C for 80 minutes, and the electrophoresis tank and sample comb were immersed in 2% hydrogen peroxide for 30 minutes.
[0027] C. Material: switchgrass leaves and stems
[0028] D. Extraction of total RNA:
[0029] (1) Sample grinding: Take about 0.5g of fresh switchgrass leaf or stem material in a mortar, add 3 3mm tungsten carbide grinding beads, add liquid nitrogen and quickly grind, repeat several times until pulverized, and the grinding time is controlled within 1min, add the ground powder to the pre-cooled 1.5mL EP tube;
[0030] (2) Nucleic acid release: Add 1 mL of the prepared RNA extraction solution to the centrifuge tube in step (1), shake w...
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[0046] Example 2
[0047] A. Preparation of experimental drugs: RNA extract, volume fraction of 0.1% DEPC water, volume fraction of 80% ethanol (prepared with 0.1% DEPC water).
[0048] B. Treatment of experimental supplies: After the utensils are soaked in 0.1% DEPC water overnight, sterilize at 120°C for 100 minutes, and the electrophoresis tank and sample comb are soaked in 3% hydrogen peroxide solution for 50 minutes.
[0049] C. Material: switchgrass leaves and stems
[0050] D. Extraction of total RNA:
[0051] (1) Sample grinding: Take about 1g of fresh switchgrass leaf or stem material in a mortar, add 5 3mm tungsten carbide grinding beads, add liquid nitrogen and quickly grind, repeat several times until grinding into powder, the grinding time is controlled at 1min , Add the ground powder to the pre-cooled 1.5mL EP tube;
[0052] (2) Nucleic acid release: Add 1 mL of the prepared RNA extraction solution to the centrifuge tube in step (1), shake well to make it evenly mixed, a...
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