30 results about "Genetic function" patented technology

The invention provides a signal lamp timing evaluation and real-time adjustment method. The method comprises steps: initialization is carried out, and related information is acquired according to an intersection number; a lane group is defined, and under a certain phase, all lanes with the right of way in the same road section are defined as one lane group; a core release lane group under the current phase is found out, and if all phases of a lane have the right of way, the lane is removed from the lane group; a phase key lane is found out; the theoretical minimum period and the maximum period are judged; the saturation of each phase key lane is judged; a penalty function for the period is determined; and a planning function under a non oversaturation phase is determined, a genetic function is called to solve the optimum solution of the planning function, and finally, according to the optimum solution result, based on the period and on the premise of green lamp protection, and the signal lamp is adjusted dynamically.

The invention discloses application of a phiC31 recombinase system and a piggyBac transposon and a fixed point transgenetic system of silkworm and a preparation method of the fixed point transgenetic system. According to the invention, the phiC31 recombinase system and the transgenic technology for mediating the silkworm to transform by the piggyBac transposon are combined; first, two attB sites or attP sites which are arranged in the same direction are connected into a piggyBac transposon carrier; the carrier in transformed into silkworm; then, single copy transgenetic silkwork is screened to be used as a target system; then, the target system is transformed by using the phiC31 recombinase and a carrier, wherein target gene expression frames are anchored at the two ends of the carrier by the attB sites or attP sites which are arranged in the same direction; and thus the target gene expression frames are transformed to the place between fixed points, namely the two attB sites or attP sites of the piggyBac transposon carrier, thereby realizing the purpose of fixed point transgene of silkwork. The method is simple to operate and provides a powerful tool for genetic function research for silkworm.

The invention provides a dynamic signal lamp assessment method based on a genetic algorithm, and the method comprises the steps: carrying out the initialization, and obtaining related information through an intersection number; defining a lane group, wherein all lanes with the passing right at one phase at the same segment are defined as one lane group; finding a core permission lane group at a current phase, wherein the lane is removed from the lane group if the lane has the passing right at all phases; finding the key lane at the phase; determining a theoretical minimum period and a theoretical maximum period; determining the saturation of the lane at each phase; determining a punishment function for the period; determining a programming function at a non-oversaturation phase, carrying out the call of a genetic function to solve an optimal solution of the programming function. The invention also proposes a dynamic signal lamp assessment system based on the genetic algorithm.

This invention publishes a new recombined framework plasmid of adenovirus vector and its application. The framework plasmid constructed by this vector can be used to obtain Ad5F35 recombined adenovirus containing foreign genes quickly, efficiently and easily, with a significantly promoted yield. The technological realization of this invention is to replace the spiked gene sequence coded with Ad5 in pBHGlox (delta) E1, 3Cre framework plasmid with spiked gene sequence coded with Ad35. It has wide application in packaging Ad5F35 recombined adenovirus vector containing foreign genes quickly, steadily and proliferously, and infecting target cells such as malignant tumor cells, human peripheral blood cells and hematopoietic stem cells which are usually difficult to infect with conventional virus vectors, and is further applicable in the field of genetic therapy, research on genetic function, antisense and bacterin development.

The invention discloses a security early-warning model based on a genetic wavelet neural-network. The model includes the following steps: 1) carrying out fuzzification of input data; 2) determining the layer number of the genetic wavelet neural-network and the node numbers of an input layer, an output layer and hidden layers; 3) carrying out information encoding; 4) carrying out population initialization; 5) carrying out fitness function calculation; 6) carrying out a selection operation; 7) carrying out a genetic operation; 8) selecting an optimal individual for decoding; 9) carrying out training according to parameters of the BP network set in the first step; 10) optimizing the wavelet neural-network; and 11) optimizing a genetic function. According to the security early-warning model ofthe invention, a structure of the wavelet neural-network is simpler, a convergence speed is higher, the neural network is enabled to have higher learning ability through using wavelet basis functionsas neurons of the neural network, precision is higher, immediate, highly-efficient, scientific, accurate and early warning on a chemical production system is realized, and chemical production accident appearing is avoided.

Provided are methods, kits and arrays for carrying out relative measurement of an analyte of interest in a biological sample. As specifically exemplified, there is an array of stabilized, desiccated cDNA preparations, each at a defined location within the array, where those cDNAs were prepared from cells treated with a particular condition believed to modulate at least one gene of interest. Detection can be via Real Time Polymerase Chain Reaction using an appropriate reaction mixture and primers specific for a coding sequence of interest, and a greater relative amount of a RT PCR product from a control preparation reflects greater gene expression in response to the test condition whereas a lower amount of RT PCR product reflects an inhibitory effect on expression of the coding sequence of interest as a result of the application of the test condition.

The invention discloses an agrobacterium-mediated dendrocalamus latiflorus transformation method which comprises the steps of preparing explants, preparing an agrobacterium infected bacteria solution, infecting, screening, inducing of adventive buds, and inducting and rooting. agrobacterium is utilized to infect explant at stem apex of dendrocalamus latiflorus, co-culture is performed in a NB culture medium containing 2 mg/L, 2,4-D and 200 mu M acetosyringone, and steps such as screening are performed to finally obtain transgenic dendrocalamus latiflorus. The agrobacterium-mediated dendrocalamus latiflorus transformation method utilizes an agrobacterium-mediated efficient transformation system to firstly establish a transgenic system, which takes dendrocalamus latiflorus nutritive organs as start, of dendrocalamus latiflorus, and the system can overcome the obstacle that the conventional breeding method cannot be applied to bamboo, and provides premise for molecular breeding of dendrocalamus latiflorus through the transgenic technology. Through the method, technical support is provided for researching genetic functions of bamboos in the basic research field.

The invention relates to a method to knock out goat EDAR (ectodysplasin-areceptor) gene via CRISPER-Cas9 (clustered regularly interspaced short palindromic repeats and CRISPR associated protein 9) system mediation; the method comprises: constructing two gRNA expression vectors based on CRISPER-Cas9 according to goat's EDAR gene sequences, transferring optimized CRISPER-Cas9 vectors and the constructed gRNA expression vectors jointly into fibroblasts of a goat fetus to obtain goat cells with EDAR gene knocked out. The CRISPER-Cas9-mediated targeting vectors constructed herein provide a simple, quick and safe means to knock out goat EDAR gene. The method involves no selection marker genes during cell line screening, the safety of transgenic animals is improved greatly, and the method is of great value to the genetic breeding of goat and genetic function studies.

Transgenic silencing is a little understood process by which genes introduced into plants are turned off or silenced. Genetic screens were designed to identify corn mutants with reduced gene silencing activity. Such mutant corn lines include Mop1-1; Mop1-2EMS; Mop2-1, mop3-1; CC2343, rmr1-1; rmr1-2; rmr2-1; rmr6-1; rmr7-1; rmr7-2; rmr8-1; rmr9-1; Mop1-4; Mop1-5; and rmr11-1 and seeds derived therefrom, the plants are useful for corn breeding programs to produce inbred and hybrid seed with reduced gene silencing activity.

The invention discloses a genetic manipulation system based on Hfx. mediterranei and pyrF genes and its application. The genetic manipulation system comprises recombinant halophilic archaea and a recombinant plasmid carrier. The recombinant halophilic archaea is prepared by defunctionalizing an encoding gene of a protein sequence of SEQ ID NO:1 in initial halophilic archaea. The recombinant plasmid carrier at least contains a replication origin required by replication of Escherichia coli, an expression cassette for screening resistance selectable marker genes of Escherichia coli transformants, an expression cassette of the encoding gene of the protein sequence of SEQ ID NO:1, and polycloning sites for allowing exogenous genes to insert. The genetic manipulation system can be used as a high-efficiency gene knockout system, and can carry out extensive genetic function research and metabolic pathway illumination on Hfx. mediterranei. According to the experiment, the frequency of positive recombinants obtained from genetic transformation by using the inventive system is greatly improved.

A select technology for the gene referring to schizoid. The technology makes mouse with schizoid be as mode, combining with nucleic acid synthesis technology and animal behaviour test. It can be used for researching genetic function of desease and developing medicine.

The invention discloses a marine actinomycete genome library and a construction method thereof. The construction method includes: extracting DNA of marine actinomycete S.arenicola CNS-205; adopting a syringe needle of an injector to randomly shear the extracted DNA; adopting terminal repairase to repair the randomly shorn DNA, and connecting with a carrier; adopting packaging protein to package into bacteriophage, and infecting a host cell to obtain the genome library of the marine actinomycete S.arenicola CNS-205. The invention aims to construct the genome library by taking the marine actinomycete S.arenicola CNS-205 which is rare for the convenience of heterologously expressing a secondary metabolism product having biological activity in the strain to biologically synthesize related gene clusters and analyzing genetic functions of the gene clusters so to apply metabolic engineering or combinatorial biosynthesis methods in compound structure modifying.

The invention discloses a method for preparing a positive oncomelania of schistosoma. The method comprises the following steps: co-culturing myracidium incubated by schistosoma eggs and sf9 cells to obtain mother sporocyst developed by the myracidium; injecting the mother sporocyst into a negative oncomelania body under microscopy; and continuously feeding the oncomelania containing injected mother sporocyst, and detecting whether the oncomelania is positive or not to obtain the positive oncomelania of the schistosoma. By the method for preparing the positive oncomelania of the schistosoma, cercaria released by the cultured positive oncomelania has the same infectivity and the same biological characteristics with the schistosoma. The method provides an efficient and convenient in vitro manual operation method for studying the genetic functions of the schistosoma.

The present invention relates to a method of detecting genetic variants, including genetic variants associated with drug resistance, cancer and genetic dysfunctional diseases.

The invention uses a CRISPR/Cas9 system to mediate completion of fixed-point knockout of a goat KRTAP13-1 gene. According to a KRTAP13-1 gene sequence of a goat, a Cas9/gRNA targeting vector based onthe CRISPR/Cas9 system is constructed by the research; an optimized Cas9/gRNA coexpression knockout vector is transfected into a goat fetus fibroblast to obtain a KRTAP13-1 gene fixed-point knockout cell strain. The knockout vector constructed by the invention based on the CRISPR/Cas9 system provides a simple, fast and safe approach for the fixed-point knockout of the goat KRTAP13-1 gene. The method does not involve any selective marker gene in a cell strain screening process, thereby greatly improving safety of transgenic animals, and has important value to the study of the genetic function and genetic breeding of the goat.

The invention belongs to the technical field of biology and particularly relates to a multi-gene combined editing and screening method for microorganisms. A plurality of recombinant vectors are transformed at one time, each recombinant vector carries one or more target sites, a plurality of target genes and a plurality of target sites can be edited at the same time through one round of operation, according to a random combination principle, strains containing a single gene, different combinations of two genes, different combinations of three genes and different combinations of multiple genes can be rapidly constructed, and a strain library of multi-gene combined editing is obtained. Compared with a conventional method for sequentially editing a plurality of genes round by round, the method is simple to operate, has high efficiency, is time-saving and labor-saving, and has a wide use prospect. For example, on the basis of the combined library, screening of microorganisms with excellent shapes is promoted, functions of homologous or same family genes are favorably researched, and the microorganisms are conveniently modified to obtain new genetic functions and characteristics.

The present invention provides a conjugate comprising an antibody or antibody fragment targeting an exosome surface antigen, and an inhibitor of a gene or an expression product thereof, wherein the antibody or antibody fragment and the inhibitor of a gene or an expression product thereof are covalently bonded either directly or via a linker, or are non-covalently bonded.