Application of substance for regulating level of NLRP6 gene and/or expression product thereof

A technology for expressing products and genes, which is applied in the field of substances that regulate the level of NLRP6 gene and/or its expression products, can solve the problems of no relevant reports on adipocyte differentiation and metabolic regulation mechanism, and achieve the effect of promoting adipocyte differentiation

Active Publication Date: 2021-11-12
XINXIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Studies on NLRP6 mostly focus on infectious inflammation and the role of NLRP6 in the gastrointestinal tract, and there are no related reports on the regulation mechanism of adipocyte differentiation and metabolism

Method used

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  • Application of substance for regulating level of NLRP6 gene and/or expression product thereof
  • Application of substance for regulating level of NLRP6 gene and/or expression product thereof
  • Application of substance for regulating level of NLRP6 gene and/or expression product thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Materials: C57 WT mice and NLRP6 KO mice were purchased from Saiye Biotechnology Co., Ltd.

[0025] DMEM medium (Gibco), fetal bovine serum (FBS), gentamicin, 1 μM dexamethasone, 0.5 Mm IBMX, 5 μg / mL insulin.

[0026] 2. Method

[0027] 2.1 Adipocyte isolation

[0028] 2.1.1 Before the experiment, put the items required for this experiment into the ultra-clean workbench, turn on the ultraviolet light for 30 minutes, and turn on the water bath to preheat to 37°C.

[0029] 2.1.2 After the ultraviolet irradiation is sterilized, turn on the wind speed and lighting options, and wipe the countertop with 75% medical ethanol.

[0030] 2.1.3 Cut out the gonadal fat of 4-week-old C57 WT mice or NLRP6 KO mice respectively.

[0031] 2.1.4 Wash the gonadal fat of WT or KO mice in three small petri dishes containing 2mL of PBS in turn.

[0032] 2.1.5 Put it into a small Petri dish containing 2mL of collagenase mixture, cut the tissue fully with scissors, collect it into a 15mL...

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Abstract

The invention relates to the technical field of genetic functions and applications, in particular to application of a substance for regulating a level of an NLRP6 gene and / or an expression product thereof. According to the application, researches find that the NLRP6 gene regulates and controls fat cell differentiation and lipid droplet fusion, the fat cell differentiation rate of an NLRP6-gene-knockout mouse is obviously increased compared with that of a wild mouse, and lipid droplets are more easily fused. A research result has a great application prospect in the aspects of occurrence, prevention, treatment and the like of chronic fat inflammation and related metabolic diseases.

Description

technical field [0001] The present invention relates to the technical field of gene function and application, in particular to the use of substances that regulate the level of NLRP6 gene and / or its expression products. Background technique [0002] Adipose tissue is mainly composed of adipocytes, interstitial fibroblasts, and precursor cells that form energy storage organs in the form of triglycerides (synthesized from fatty acids), packaged into lipid droplets. Adipocyte differentiation is now recognized as a dynamic, multimolecular process of differentiation from preadipocytes to mature adipocytes. It was also found that the role of fat cells is not only to store energy, but also has endocrine function, which can regulate the secretion, absorption and utilization of various hormones, cytokines and nutrients by secreting a variety of factors, and is considered to be the key to the homeostasis of systemic metabolism. important regulator. Adipocytes can release biologically...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61P3/04A61P29/00A61P3/10A61P9/00
CPCA61K45/00A61P3/04A61P29/00A61P3/10A61P9/00
Inventor 于莉莉李滟镕冯志伟任峰
Owner XINXIANG MEDICAL UNIV
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