213 results about "Mus spretus" patented technology

The invention discloses a construction method and application of an NMD escape model of an Alport syndrome mouse. A non-human animal model carrying Col4a5 gene c.4432delG frame shift mutation is prepared on the basis of a CRISPR / Cas9 gene editing technology. The method comprises the following steps: co-injecting gRNA of a 49 exon of a targeted Col4a5 gene, homologous recombinant donor oligonucleotide containing c.4432delG mutation and Cas9 nuclease into a mouse fertilized egg, and carrying out embryo transplantation to obtain an F0-generation mutant mouse; a mutation site is verified by combining PCR (Polymerase Chain Reaction) with sequencing, and a stably inherited mutation line is established through two generations of breeding. Through verification, the model accords with pathological characteristics of the Alport syndrome, can stably simulate typical clinical manifestation and pathological characteristics of the human X-linked Alport syndrome, and can be used as an important tool for research of the Alport syndrome.

The invention relates to the technical field of animal experiment equipment, and discloses an isolated culture device for mouse sweet water preference test in an IVC cage, which comprises a shell cover, the partition plate is fixedly installed on the inner side wall of the shell cover, and an inner cavity of the shell cover is divided into a living area and a functional area through the partition plate; the sliding cover is arranged in the living area of the shell cover and used for containing solid feed, and multiple sets of feeding trough openings which are arranged at equal intervals are formed in the bottom of the sliding cover; according to the device, an innovative cage-in-cage design is adopted, a single cage is converted into a plurality of independent experiment units on the premise that an existing IVC system is not changed, and cage position resources are greatly saved. The functions of isolation, double-bottle water drinking and independent feeding are integrated, and convenient operation is achieved through the pull-out type fixing plate and the sliding cover plate. The centrifugal tube is adopted to replace a special water bottle, so that the cost is reduced, the SPF environment is kept in the whole process, data accuracy and reliability are ensured, and the traditional SPT test problem is effectively solved.

The spliced sleep deprivation mouse cage comprises a plurality of square cage bodies, a tray and a food box, the square cage bodies are transparent structural members, the top of each square cage body is provided with an organism inlet and at least one square notch, the square notches are formed in the side edges of the square cage bodies, and the food box is arranged on the tray. A plurality of water leakage holes are evenly formed in the bottoms of the square cage bodies at intervals, clamping grooves are formed in the tray, the square cage bodies can be spliced and embedded into the clamping grooves, circulation gaps are formed between the bottom walls of the square cage bodies and the inner bottom wall of the tray, and a water injection opening is formed in the side wall of the tray. When the sides, provided with the square notches, of any four square cage bodies are spliced, a containing groove communicated with the four square cage bodies is formed, and the food box is arranged in the containing groove. The splicing type sleep deprivation mouse cage can effectively deprive the sleep of mice, the problems of poor sleep deprivation and wire entanglement caused by mixed feeding of the mice are solved, the water level height in each mouse cage is consistent, and meanwhile, the water quality is convenient to replace.

The invention provides a method for establishing a mouse postoperative delirium model under a simulated high altitude urgent entry environment, and relates to the technical field of animal model establishment. According to the method for establishing the model for simulating postoperative delirium of the mouse in the sudden entering plateau environment, a control group (group C), a plateau group (group H), a POD model group (group P) and a plateau POD model group (group HP) are included, behavior changes of the mouse are evaluated through a Y maze experiment, then the mouse is killed, brain tissue is taken, and the mouse is subjected to postoperation delirium simulation in the sudden entering plateau environment. The mouse hippocampus tissue structure damage change is observed through morphological methods such as Nissl staining and a transmission electron microscope, meanwhile, blood is taken through mouse eye sockets, the concentration of inflammatory related indexes IL-6 and TNF-alpha in serum is measured through an ELISA method, and the stability and repeatability of model establishment are known. The plateau postoperative delirium mouse model prepared by the invention can generate obvious damage to brain tissues and neurological functions, and is high in operability and good in repeatability.

A primer pair, method, and use for rapidly identifying newborn nude mice. On the basis that a deletion mutation occurs in the base G at position 337 of a coding region of the Foxn1 gene in nude mice, a primer pair introducing an enzyme digestion site is designed according to sequence characteristics. Using the genome of a mouse to be tested as a template, the Foxn1 gene is amplified according to a PCR-RFLP method. The PCR product is then digested with a SmaI restriction endonuclease, and the digested product is genotyped by agarose gel electrophoresis. In addition, a reaction system that can be combined with lateral flow technology is screened, thereby achieving instant detection of the newborn nude mice. The method has the characteristics of simple operation, short time consumption, low cost, instant detection, etc.

The invention relates to the technical field of medicines, in particular to novel application of galactooligosaccharide. The novel use of galactooligosaccharide inhibits the formation of gallstone. Main reagents and materials are galactooligosaccharide; a mouse calculus model feed; the experimental animals are derived from animal varieties and strains: mice, C57BL / 6J; sex: male; week age: 4 week age; the body weight is 16-20 g; the experimental environment is SPF environment feeding, and the feeding condition is that the room temperature is 20-26 DEG C; the relative humidity is 40-70%; automatic illumination is achieved, and light and shade are alternated for 12 h; the drinking water is RO membrane reverse osmosis sterile water; the feeding density is defaulted to be 4 per cage, and the padding is replaced once a week; the experimental process comprises the following steps: starting an experiment after experimental feeding of all mice for one week, and randomly dividing 36 5-week male C57BL / 6J mice into three groups, namely 12 mice in each group. The invention provides a new application of galactooligosaccharide, which can inhibit the formation of gallstone and provides a new thought for preventing the occurrence of the gallstone.

The application relates to the technical field of animal activity behavior monitoring, and discloses an animal behavior activity monitoring device, which comprises a supporting seat, supporting columns are symmetrically arranged at the bottom end of the supporting seat, a supporting frame is fixedly connected to the top end of the supporting seat, a monitoring body is arranged at the top end of the supporting frame, a receiving cavity is arranged in the supporting seat, a discharging pipe, which is in communication with the receiving cavity, is arranged at the bottom end of the supporting seat, an annular track is arranged at the top end of the supporting seat, an opening is arranged at the bottom end of the annular track, and a supporting and discharging assembly is arranged in the opening; the two feeding frames can be driven to move in the annular track, the feeding frames can push the mice to move to achieve sleep deprivation of the mice, the problem that, when a traditional feeding frame with a cuboid structure is fixedly arranged, the mice are driven to move only by a deprivation rod and the sleep deprivation of the mice is interrupted due to a gap caused by the deprivation rod is solved, and the sleep deprivation of the mice can be realized continuously.

The invention belongs to the technical field of animal model construction, and particularly relates to a mouse model based on alpha-synuclein mutation and a construction method and application thereof. The mouse Snca gene is subjected to site-specific modification and mutates at the site S129A, S129D or Y125F, an alpha-syn mutation animal model which does not need exogenous induction and can naturally develop typical PD pathology and behavior phenotypes is constructed, and the limitation of an existing model in the aspects of pathology reduction degree and experiment controllability is broken through.

The application discloses a mouse and Mongolian gerbil universal immunization sampling fixing device, and relates to the field of experimental animal fixing devices.The device comprises a supporting base, a bearing support is arranged on the front end surface of the supporting base, a sampling fixing plate is rotatably arranged on the outer surface of the bearing support, guide sliding grooves are symmetrically arranged on the upper surface of the sampling fixing plate, movable blocks are movably arranged in the guide sliding grooves, and a limiting sleeve is fixedly arranged on the front surface of the sampling fixing plate and extends from one end of the movable blocks.The synchronous transmission structure of the supporting disc, the first connecting plate and the second connecting plate drives the movable blocks on the two sides to synchronously move towards each other or in the opposite direction along the guide sliding grooves, and the movable blocks are matched with the positioning support plates which can slide in the limiting sleeve, so that the horizontal spacing and the longitudinal extension length of the limb fixing points can be simultaneously adjusted, the adjustment range completely covers the size difference of adult mice and adult Mongolian gerbils, and two special fixing devices are not needed, so that the universality of the device is improved compared with the prior art.

The invention discloses a construction method of a COL4A5-K229X point mutation X-linked Alport syndrome mouse model. The construction method comprises the following steps: aiming at c.685Agt of a No.12 exon of a mouse COL4A5 gene; carrying out T point mutation, and designing and preparing Cas9 mRNA, gRNA and a donor vector; the components are mixed and then microinjected into fertilized eggs of a C57BL / 6J mouse to obtain an F0-generation mouse; identifying the genotype through PCR (Polymerase Chain Reaction) amplification and Sanger sequencing, and screening positive mice; mating the positive F0-generation mice with the wild-type mice, and breeding F1-generation and subsequent generations; the phenotype of the model is further verified through qPCR, biochemical analysis, light microscopic examination, transmission electron microscope and immunofluorescence. The model constructed by the invention shows hematuria, proteinuria, azemia, podocyte loss and irregular thickening and layering of glomerular basement membrane, is consistent with phenotypes of human XLAS patients, and provides an animal model tool for analyzing pathogenesis and developing treatment strategies.

The invention discloses a construction method and application of an enterovirus 71 type susceptible transgenic mouse model, and belongs to the technical field of biology. The method comprises the following steps: up-regulating or enhancing or improving the content or / and activity of placenta specific protein 8 in a receptor mouse to obtain a mouse overexpressing the placenta specific protein 8, hybridizing the mouse overexpressing the placenta specific protein 8 with a wild mouse to obtain an F1-generation heterozygote mouse, a homozygote mouse obtained after the F1-generation heterozygote mice are hybridized with each other is an enterovirus 71 type susceptible transgenic mouse model. The application has the advantages that not only is the susceptibility of mice to EV71 increased, but also a proper animal model is provided for exploring the important function of Plac8 in the EV71 infection process.

The application discloses a method for constructing a CRBN gene humanized mouse model, which comprises replacing a knockout region of a mouse CRBN gene with a knock-in region of a human CRBN gene, so that the CRBN gene humanized mouse model is constructed, and the CRBN gene of the CRBN gene humanized mouse model is subjected to amino acid substitution of S369C, V380E, I391V and E431D. The application replaces a key functional domain of a mouse endogenous CRBN gene with a corresponding sequence of a human CRBN gene, so that the mouse can express a functional human CRBN protein, thereby solving the problem of lack of sensitivity of a wild-type mouse to a CRBN-dependent drug. The model provides an ideal preclinical experimental platform for studying the efficacy, toxicity and mechanism of action of a CRBN-related drug.

Non-human animals, and methods and compositions for making and using the same, are provided, wherein said non-human animals comprise a humanization of an endogenous cluster of differentiation (CD) gene, in particular a humanization of a CD47 gene. Said non-human animals may be described, in some embodiments, as having a genetic modification to an endogenous CD47 gene so that said non-human animals express a CD47 polypeptide that includes a human portion and a non-human portion (e.g., a murine portion).

The invention discloses a construction method of a glomerular IgA deposition mouse model, and relates to the technical field of animal model development and glomerular IgA dyeing. Comprising the following steps: obtaining Cas9 mRNA (messenger ribonucleic acid), guideRNA (ribonucleic acid) and donor DNA (deoxyribonucleic acid) in an in-vitro transcription manner; the method comprises the following steps: carrying out microinjection on Cas9 mRNA (messenger ribonucleic acid), gRNA (guide ribonucleic acid) and donor DNA (deoxyribonucleic acid) into fertilized eggs of a C57BL / 6J mouse; carrying out genotype identification on the F0 generation of birth mice through PCR (Polymerase Chain Reaction) amplification and sequencing; mating the F0-generation positive mouse with a wild type C57BL / 6J mouse, and breeding to obtain an F1-generation mouse; and spontaneous glomerular IgA deposition appears in 24 weeks after the F1 generation heterozygote mouse is born. According to the method, accurate mutation is introduced by utilizing a homologous recombination repair mechanism to construct a glomerular IgA deposition model, the efficiency is high, and the off-target risk is low.

The invention discloses primers and a method for detecting Corynebacterium bovis and application of the primers and the method in detection of pathogenic bacteria of immunodeficient mice. The primers comprise an upstream primer and a downstream primer aiming at 16S rRNA (ribosomal Ribonucleic Acid) genes of the Corynebacterium bovis. The invention establishes a qPCR molecular detection method for the pathogenic bacteria Corynebacterium bovis of the immunodeficient mice, and the qPCR molecular detection method has the advantages of strong specificity, high sensitivity, high detection efficiency and the like, and can quickly and accurately detect the Corynebacterium bovis in the immunodeficient mice and the environment. The establishment of the detection method is beneficial to timely discovery and control of the bacterium, and has important significance for guaranteeing the environmental safety of personnel and the health of experimental animals. In future experimental animal quality control and related scientific research work, the qPCR detection method is expected to be widely applied, and powerful technical support is provided for further deep research on biological characteristics and pathogenic mechanisms of corynebacterium bovis.

1. Name of the product in this design: Mouse tail blood collection fixation device. 2. Purpose of this design: Used as a fixation device for blood collection from the tail of mice. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1.

The application discloses a mouse vitiligo induction model based on chronic stress factors, wherein different kinds of stress factors capable of triggering stress response of animals are applied to stimulate the mouse periodically within a certain time period, so as to prevent the mouse from producing habitual response to the stress source and to promote the mouse to become white and the tail epidermis to be flaky. The technical method is different from the existing vitiligo model method, is based on the mechanism of occurrence and development of vitiligo observed in the clinic, and promotes the mouse to truly develop the vitiligo phenomenon from the whole instead of simulating the disease evolution locally. Compared with the existing vitiligo promoting mechanism, the effect is more stable and the authenticity is higher. The technical method is based on the objective fact that the clinical vitiligo patients often combine anxiety and depression to design the stress factors, and considers that the stress factors and the occurrence and development of vitiligo may be inter-caused and viciously circulate, namely, the adverse life events as the stress factors cause the damage and dysfunction of the melanin synthesis system at the micro level and form the vitiligo change at the macro level.

The invention discloses a method for detecting mouse bronchial Bordetella by using a qPCR (quantitative polymerase chain reaction) technology instead of a traditional sacrificial sentinel mouse, and a specific primer and probe combination comprises a forward primer sequence: 5 '-CGTTGTCGGCTTTCGTCTG-3', as shown in SEQ ID NO.1; the sequence of a reverse primer is 5 '-ACTATACGTCGGAAATCTGTTTG-3', and the sequence of the reverse primer is as shown in SEQ ID NO. 2; and a probe sequence: 5 '-FAM-CGGGCCGATAGTCAGGGCGTAG-BHQ1-3', and the probe sequence is as shown in SEQ ID NO. 3. The method disclosed by the invention has the beneficial effects that an analysis method suitable for detecting the bronchial Bordetella in the animal cage environment sample is established, the bronchial Bordetella in the environment sample can be rapidly and accurately detected and quantified by the method, and a technical support is provided for pathogen pollution control.

The application relates to a mouse model construction method for psoriasis research based on SBSN 600 lysine dihydroxyisobutyryl modification, which comprises the following steps: first step, constructing a plasmid comprising a DNA fragment of a SBSN gene sequence, wherein 600 sites of the SBSN sequence are mutated from lysine (K) to threonine (T); second step, culturing mouse embryonic stem cells; third step, electrically transforming the embryonic stem cells; fourth step, drug screening; fifth step, culturing the embryonic stem cells screened in the fourth step; sixth step, injecting the embryonic stem cells cultured in the fourth step into mouse blastocysts, then culturing the mouse blastocysts, and transplanting the cultured blastocysts into the uterus of a pseudopregnant female mouse, wherein the mouse delivered by the female mouse is a chimera mouse; and seventh step, breeding the chimera mouse and identifying the mouse genotype through PCR.

The invention provides an NOD / shiLt mouse genotype fluorescent quantitative identification method and application, and relates to the field of experimental animal genetic quality control. Comprising the following steps: acquiring a to-be-detected mouse genetic identification sample, and performing DNA extraction treatment on the mouse genetic identification sample to form a genome DNA template; according to the fluorescent quantitative identification method for the genotype of the NOD / shiLt mouse and the application of the fluorescent quantitative identification method, a real-time fluorescent quantitative polymerase chain reaction system containing a specific primer pair, an insertable fluorescent dye premixed solution and a standard substance series is constructed, so that accurate quantitative detection on a specific deletion sequence of a No.3 chromosome of the NOD / shiLt mouse and a reference gene GAPDH is realized. According to the present invention, the linear relationship between the Ct value and the template copy number is established through the standard substance series, and the quality determination of the amplification curve, the melting curve and the reference gene is combined, such that the NOD / shiLt mouse genotype fluorescence quantitative identification method can stably and rapidly identify whether the mouse genomic DNA contains the NOD / shiLt mouse genotype.

The application relates to a mouse metabolic behavior detection intervention device and an intervention method, and belongs to the technical field of animal experiment equipment. The device comprises a shell, an intervention cabin and a metabolism cabin are arranged in the shell, the intervention cabin comprises a cabin body located in the shell, an inducer for guiding experimental mice to spontaneously enter the cabin body is arranged at one end of the cabin body away from an entrance end of the cabin body, a self-motion mechanism is located between the inducer and the entrance end of the cabin body, is used for carrying the mice and generating reciprocating motion in response to spontaneous movement of the mice along the length direction of the cabin body, a monitoring mechanism is located at the periphery of the cabin body, is used for monitoring the position, state and motion track of the experimental mice in the cabin body, and a sealing mechanism is located at the entrance end of the cabin body and is used for opening or closing the entrance of the cabin body according to a detection signal of the monitoring unit. The mice spontaneously enter the intervention cabin through the inducer and autonomously run on the conveying belt, traditional forced motion means such as electric shock, a push rod and high-pressure airflow are completely abandoned, and the accuracy of monitoring data is improved.

The present disclosure relates to a method for constructing a mouse model with short telomere. Specifically, the present disclosure obtains fertilized eggs by fertilizing the sperms and eggs of mice in vitro, and the fertilized eggs are cultured to the blastocyst stage in vitro and then transferred into the surrogate female mouse for development, thereby producing mice with short telomere. The method of the present disclosure does not require gene editing, has a short modeling cycle, and the effect is reliable and stable. By changing only the environment during embryo transfer, the telomere elongation process in the embryo is interfered, so as to successfully construct a progeny model with short telomere. There is no significant effect on the reproductive rate of female mice. Therefore, the present disclosure can provide a method for constructing a mouse model with short telomere for exploring the mechanism of telomere shortening and studying telomere-related phenotypes such as aging.

The application provides a method for constructing a Bmp4 gene knock-in mouse model, and the method comprises the following steps: obtaining sgRNA12 shown in SEQ ID NO. 3; mixing the sgRNA12, Cas9 mRNA and a targeting vector shown in SEQ ID NO. 1, and then microinjecting into a mouse zygote to obtain F0 generation mice; selecting F0 generation positive mice in genotype identification results of the F0 generation mice, and mating the F0 generation positive mice with wild type mice to obtain F1 generation mice with stable genotypes; and screening gene targeting mice with correct recombination, and mating the gene targeting mice with retinal specific knock-out tool mice Six3-Cre to obtain Bmp4 gene knock-in model mice. The method has high gene editing efficiency, and the transgenic mouse can highly express human Bmp4 protein.

The present invention discloses a method for preparing a murine model of Staphylococcus aureus pneumonia and model evaluation, and the steps are as follows: Step 1: in vitro labeling of Staphylococcus aureus; Step 2: establishment of a murine model of Staphylococcus aureus pneumonia. By adopting the method of exposing the trachea and quantitatively aerosolizing bacteria through the oral trachea using a murine tracheal quantitative aerosol nebulization device, the amount inhaled by the mice can be controlled at the same level, with smaller errors in each group and higher quality of model establishment; the operation difficulty is small, the success rate of model establishment can be effectively improved, and the damage to animals is small. By observing the lung imaging of mice in vivo through a near-infrared imaging system, if obvious and uniform near-infrared fluorescence images are observed in the lungs of the mice, it indicates that the model establishment is successful. It can know whether the model is successfully established in the first time, avoiding lag. Moreover, this evaluation method does not require a series of invasive operations to extract samples and subsequent series of detections. The method is simple to operate and takes a short time.

The invention discloses an operation table for dissecting rats and mice, and relates to the technical field of mouse dissecting tables, the operation table comprises an experiment assembly and a laparotomy assembly, and the experiment assembly comprises a working plate; the laparotomy assembly comprises a rotating frame, a deformation plate and a clamping plate; the rotating frame is rotationally connected to the working plate through a friction hinge and is used for placing a mouse; during laparotomy and mouse observation, the rotating frame can drive the whole mouse to rotate, and the wound orientation is adjusted; a clamping plate is arranged at one end, far away from the rotating frame, of the deformation plate; the deformation plate is made of a bendable iron material, and the clamping plate is used for clamping and unfolding the epidermis of a mouse; according to the device, an operator can adjust the angle and the distance between the two clamping plates at will through the deformation plate capable of being manually bent, so that the device can be perfectly attached to mice of various body types from small size to large size, the problem that the belly is not fully lifted due to too loose clamping or the chest is pressed due to too tight clamping is solved, and a stable foundation is provided for anatomy operation.

A mouse model with conditional knockout of a vitamin D-binding protein gene, and the use thereof. A method comprises the following steps: S1, performing flox modification on the mouse vitamin D-binding protein gene to obtain F0-generation positive mice having undergone the flox modification; S2, mating said F0-generation positive mice with wild type mice to obtain F1-generation positive mice with preserved flox modification; S3, hybridizing the F1-generation positive mice with tissue-specific Cre tool mice to obtain heterozygous mice, and mutually mating the heterozygous mice to obtain homozygous mice; and S4, selecting the obtained homozygous mice to undergo tamoxifen injection, so as to obtain mice with conditional knockout of the vitamin D-binding protein gene.

This invention, belonging to the field of bioinformatics, discloses a cross-species prediction method for peptide-encoding sequence-dependent open-type recurrent (sORF) proteins. The method integrates protein-encoding DNA sequences from human and mouse sORF databases, Arabidopsis thaliana from the TAIR database, and some prokaryotes from the NCBI database. Positive and negative samples corresponding to each species are constructed using data filtering and negative sample generation strategies. Sequence features are extracted and the training set is screened using maximum relevance minimum redundancy and incremental selection methods to obtain the optimal feature sets corresponding to different methods and construct a peptide-encoding sORF prediction model. Parameters are optimized using a greedy grid search method to construct the optimal prediction model based on a support vector machine. The prediction model is evaluated using sensitivity, specificity, accuracy, and the Matthews correlation coefficient. This method facilitates the classification and identification of peptide-encoding sORFs and is of great significance for the study of peptide-encoding sORFs and gene annotation.

The invention discloses a method for constructing a mammalian leucopenia model based on a Crispr gene editing technology. The invention belongs to the technical field of biology, and particularly relates to a method for constructing a mammalian leucopenia model based on a Crispr gene editing technology. The construction method of the leucopenia mouse model comprises the following steps: specifically mutating an H4K31 site in a mouse fertilized egg cell, so that the site is changed into arginine from lysine, and the leucopenia mouse model is obtained. A corresponding gene sequence is mutated at an H4K31 site in a fertilized egg cell of the specifically mutated mouse by means of cutting of a Crispr gene editing system and a fixed-point knock-in technology of homologous recombination. By detecting the immune function regulation and control mechanism of a homozygous mouse with the H4K31 site after the homozygous mouse with the H4K31 site is prepared, the mouse shows a natural leucopenia phenotype after lysine at the H4K31 site is mutated into arginine.

The invention discloses a construction method and application of a DNAJB6a gene specific knockout mouse model, and belongs to the technical field of gene engineering. Comprising the following steps: S1, designing gRNA sequences aiming at ninth to tenth exon regions of the mouse DNAJB6 gene; s2, in vitro transcription is carried out to prepare Cas9mRNA and gRNA; s3, carrying out microinjection on Cas9mRNA and gRNA (guide Ribonucleic Acid) into a fertilized egg of the mouse; s4, transplanting the fertilized ovum after injection into the body of a pseudo-pregnant female mouse to obtain an F0-generation mouse; s5, screening positive F0-generation mice through genotype identification, and mating the positive F0-generation mice with the wild type mice to obtain F1-generation heterozygote mice; s6, the F1-generation heterozygote mice are matched to obtain F2-generation homozygote mice, and the DNAJB6a gene specific knockout mouse model is established. The construction method for constructing the DNAJB6a gene knockout mouse model, provided by the invention, is simple and convenient to operate, short in construction process time and stable in genotype, and a DNAJB6a gene knockout mouse can be effectively obtained.

The invention discloses an ABPA animal model building method which comprises the following steps: building an ABPA animal model by injecting a live aspergillus fumigatus spore suspension into an airway, and selecting a mouse for adaptive feeding; a primary sensitization stage; repeating the excitation stage; a live aspergillus fumigatus spore colonization excitation stage; and establishing an evaluation model. The animal model which highly simulates the pathophysiological characteristics of human ABPA is constructed by performing sensitization and excitation in stages and in multiple modes, especially by introducing the key step of lung colonization of live aspergillus fumigatus spores; through increase of serum total IgE and aspergillus fumigatus specific IgE levels, airway eosinophilic granulocyte infiltration and high secretion of mucus, the evaluation model is successfully constructed, and a reliable experimental platform is provided for deep analysis of pathogenesis of diseases.