139 results about "Mus spretus" patented technology

The invention relates to the technical field of animal experiment equipment, and discloses an isolated culture device for mouse sweet water preference test in an IVC cage, which comprises a shell cover, the partition plate is fixedly installed on the inner side wall of the shell cover, and an inner cavity of the shell cover is divided into a living area and a functional area through the partition plate; the sliding cover is arranged in the living area of the shell cover and used for containing solid feed, and multiple sets of feeding trough openings which are arranged at equal intervals are formed in the bottom of the sliding cover; according to the device, an innovative cage-in-cage design is adopted, a single cage is converted into a plurality of independent experiment units on the premise that an existing IVC system is not changed, and cage position resources are greatly saved. The functions of isolation, double-bottle water drinking and independent feeding are integrated, and convenient operation is achieved through the pull-out type fixing plate and the sliding cover plate. The centrifugal tube is adopted to replace a special water bottle, so that the cost is reduced, the SPF environment is kept in the whole process, data accuracy and reliability are ensured, and the traditional SPT test problem is effectively solved.

A primer pair, method, and use for rapidly identifying newborn nude mice. On the basis that a deletion mutation occurs in the base G at position 337 of a coding region of the Foxn1 gene in nude mice, a primer pair introducing an enzyme digestion site is designed according to sequence characteristics. Using the genome of a mouse to be tested as a template, the Foxn1 gene is amplified according to a PCR-RFLP method. The PCR product is then digested with a SmaI restriction endonuclease, and the digested product is genotyped by agarose gel electrophoresis. In addition, a reaction system that can be combined with lateral flow technology is screened, thereby achieving instant detection of the newborn nude mice. The method has the characteristics of simple operation, short time consumption, low cost, instant detection, etc.

The application relates to the technical field of animal activity behavior monitoring, and discloses an animal behavior activity monitoring device, which comprises a supporting seat, supporting columns are symmetrically arranged at the bottom end of the supporting seat, a supporting frame is fixedly connected to the top end of the supporting seat, a monitoring body is arranged at the top end of the supporting frame, a receiving cavity is arranged in the supporting seat, a discharging pipe, which is in communication with the receiving cavity, is arranged at the bottom end of the supporting seat, an annular track is arranged at the top end of the supporting seat, an opening is arranged at the bottom end of the annular track, and a supporting and discharging assembly is arranged in the opening; the two feeding frames can be driven to move in the annular track, the feeding frames can push the mice to move to achieve sleep deprivation of the mice, the problem that, when a traditional feeding frame with a cuboid structure is fixedly arranged, the mice are driven to move only by a deprivation rod and the sleep deprivation of the mice is interrupted due to a gap caused by the deprivation rod is solved, and the sleep deprivation of the mice can be realized continuously.

The invention belongs to the technical field of animal model construction, and particularly relates to a mouse model based on alpha-synuclein mutation and a construction method and application thereof. The mouse Snca gene is subjected to site-specific modification and mutates at the site S129A, S129D or Y125F, an alpha-syn mutation animal model which does not need exogenous induction and can naturally develop typical PD pathology and behavior phenotypes is constructed, and the limitation of an existing model in the aspects of pathology reduction degree and experiment controllability is broken through.

The application discloses a mouse and Mongolian gerbil universal immunization sampling fixing device, and relates to the field of experimental animal fixing devices.The device comprises a supporting base, a bearing support is arranged on the front end surface of the supporting base, a sampling fixing plate is rotatably arranged on the outer surface of the bearing support, guide sliding grooves are symmetrically arranged on the upper surface of the sampling fixing plate, movable blocks are movably arranged in the guide sliding grooves, and a limiting sleeve is fixedly arranged on the front surface of the sampling fixing plate and extends from one end of the movable blocks.The synchronous transmission structure of the supporting disc, the first connecting plate and the second connecting plate drives the movable blocks on the two sides to synchronously move towards each other or in the opposite direction along the guide sliding grooves, and the movable blocks are matched with the positioning support plates which can slide in the limiting sleeve, so that the horizontal spacing and the longitudinal extension length of the limb fixing points can be simultaneously adjusted, the adjustment range completely covers the size difference of adult mice and adult Mongolian gerbils, and two special fixing devices are not needed, so that the universality of the device is improved compared with the prior art.

The invention discloses a construction method of a COL4A5-K229X point mutation X-linked Alport syndrome mouse model. The construction method comprises the following steps: aiming at c.685Agt of a No.12 exon of a mouse COL4A5 gene; carrying out T point mutation, and designing and preparing Cas9 mRNA, gRNA and a donor vector; the components are mixed and then microinjected into fertilized eggs of a C57BL / 6J mouse to obtain an F0-generation mouse; identifying the genotype through PCR (Polymerase Chain Reaction) amplification and Sanger sequencing, and screening positive mice; mating the positive F0-generation mice with the wild-type mice, and breeding F1-generation and subsequent generations; the phenotype of the model is further verified through qPCR, biochemical analysis, light microscopic examination, transmission electron microscope and immunofluorescence. The model constructed by the invention shows hematuria, proteinuria, azemia, podocyte loss and irregular thickening and layering of glomerular basement membrane, is consistent with phenotypes of human XLAS patients, and provides an animal model tool for analyzing pathogenesis and developing treatment strategies.

The application discloses a method for constructing a CRBN gene humanized mouse model, which comprises replacing a knockout region of a mouse CRBN gene with a knock-in region of a human CRBN gene, so that the CRBN gene humanized mouse model is constructed, and the CRBN gene of the CRBN gene humanized mouse model is subjected to amino acid substitution of S369C, V380E, I391V and E431D. The application replaces a key functional domain of a mouse endogenous CRBN gene with a corresponding sequence of a human CRBN gene, so that the mouse can express a functional human CRBN protein, thereby solving the problem of lack of sensitivity of a wild-type mouse to a CRBN-dependent drug. The model provides an ideal preclinical experimental platform for studying the efficacy, toxicity and mechanism of action of a CRBN-related drug.

The invention discloses a construction method of a glomerular IgA deposition mouse model, and relates to the technical field of animal model development and glomerular IgA dyeing. Comprising the following steps: obtaining Cas9 mRNA (messenger ribonucleic acid), guideRNA (ribonucleic acid) and donor DNA (deoxyribonucleic acid) in an in-vitro transcription manner; the method comprises the following steps: carrying out microinjection on Cas9 mRNA (messenger ribonucleic acid), gRNA (guide ribonucleic acid) and donor DNA (deoxyribonucleic acid) into fertilized eggs of a C57BL / 6J mouse; carrying out genotype identification on the F0 generation of birth mice through PCR (Polymerase Chain Reaction) amplification and sequencing; mating the F0-generation positive mouse with a wild type C57BL / 6J mouse, and breeding to obtain an F1-generation mouse; and spontaneous glomerular IgA deposition appears in 24 weeks after the F1 generation heterozygote mouse is born. According to the method, accurate mutation is introduced by utilizing a homologous recombination repair mechanism to construct a glomerular IgA deposition model, the efficiency is high, and the off-target risk is low.

The invention discloses primers and a method for detecting Corynebacterium bovis and application of the primers and the method in detection of pathogenic bacteria of immunodeficient mice. The primers comprise an upstream primer and a downstream primer aiming at 16S rRNA (ribosomal Ribonucleic Acid) genes of the Corynebacterium bovis. The invention establishes a qPCR molecular detection method for the pathogenic bacteria Corynebacterium bovis of the immunodeficient mice, and the qPCR molecular detection method has the advantages of strong specificity, high sensitivity, high detection efficiency and the like, and can quickly and accurately detect the Corynebacterium bovis in the immunodeficient mice and the environment. The establishment of the detection method is beneficial to timely discovery and control of the bacterium, and has important significance for guaranteeing the environmental safety of personnel and the health of experimental animals. In future experimental animal quality control and related scientific research work, the qPCR detection method is expected to be widely applied, and powerful technical support is provided for further deep research on biological characteristics and pathogenic mechanisms of corynebacterium bovis.

1. Name of the product in this design: Mouse tail blood collection fixation device. 2. Purpose of this design: Used as a fixation device for blood collection from the tail of mice. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1.

The application discloses a mouse vitiligo induction model based on chronic stress factors, wherein different kinds of stress factors capable of triggering stress response of animals are applied to stimulate the mouse periodically within a certain time period, so as to prevent the mouse from producing habitual response to the stress source and to promote the mouse to become white and the tail epidermis to be flaky. The technical method is different from the existing vitiligo model method, is based on the mechanism of occurrence and development of vitiligo observed in the clinic, and promotes the mouse to truly develop the vitiligo phenomenon from the whole instead of simulating the disease evolution locally. Compared with the existing vitiligo promoting mechanism, the effect is more stable and the authenticity is higher. The technical method is based on the objective fact that the clinical vitiligo patients often combine anxiety and depression to design the stress factors, and considers that the stress factors and the occurrence and development of vitiligo may be inter-caused and viciously circulate, namely, the adverse life events as the stress factors cause the damage and dysfunction of the melanin synthesis system at the micro level and form the vitiligo change at the macro level.

The invention discloses a method for detecting mouse bronchial Bordetella by using a qPCR (quantitative polymerase chain reaction) technology instead of a traditional sacrificial sentinel mouse, and a specific primer and probe combination comprises a forward primer sequence: 5 '-CGTTGTCGGCTTTCGTCTG-3', as shown in SEQ ID NO.1; the sequence of a reverse primer is 5 '-ACTATACGTCGGAAATCTGTTTG-3', and the sequence of the reverse primer is as shown in SEQ ID NO. 2; and a probe sequence: 5 '-FAM-CGGGCCGATAGTCAGGGCGTAG-BHQ1-3', and the probe sequence is as shown in SEQ ID NO. 3. The method disclosed by the invention has the beneficial effects that an analysis method suitable for detecting the bronchial Bordetella in the animal cage environment sample is established, the bronchial Bordetella in the environment sample can be rapidly and accurately detected and quantified by the method, and a technical support is provided for pathogen pollution control.

The invention provides an NOD / shiLt mouse genotype fluorescent quantitative identification method and application, and relates to the field of experimental animal genetic quality control. Comprising the following steps: acquiring a to-be-detected mouse genetic identification sample, and performing DNA extraction treatment on the mouse genetic identification sample to form a genome DNA template; according to the fluorescent quantitative identification method for the genotype of the NOD / shiLt mouse and the application of the fluorescent quantitative identification method, a real-time fluorescent quantitative polymerase chain reaction system containing a specific primer pair, an insertable fluorescent dye premixed solution and a standard substance series is constructed, so that accurate quantitative detection on a specific deletion sequence of a No.3 chromosome of the NOD / shiLt mouse and a reference gene GAPDH is realized. According to the present invention, the linear relationship between the Ct value and the template copy number is established through the standard substance series, and the quality determination of the amplification curve, the melting curve and the reference gene is combined, such that the NOD / shiLt mouse genotype fluorescence quantitative identification method can stably and rapidly identify whether the mouse genomic DNA contains the NOD / shiLt mouse genotype.

The application relates to a mouse metabolic behavior detection intervention device and an intervention method, and belongs to the technical field of animal experiment equipment. The device comprises a shell, an intervention cabin and a metabolism cabin are arranged in the shell, the intervention cabin comprises a cabin body located in the shell, an inducer for guiding experimental mice to spontaneously enter the cabin body is arranged at one end of the cabin body away from an entrance end of the cabin body, a self-motion mechanism is located between the inducer and the entrance end of the cabin body, is used for carrying the mice and generating reciprocating motion in response to spontaneous movement of the mice along the length direction of the cabin body, a monitoring mechanism is located at the periphery of the cabin body, is used for monitoring the position, state and motion track of the experimental mice in the cabin body, and a sealing mechanism is located at the entrance end of the cabin body and is used for opening or closing the entrance of the cabin body according to a detection signal of the monitoring unit. The mice spontaneously enter the intervention cabin through the inducer and autonomously run on the conveying belt, traditional forced motion means such as electric shock, a push rod and high-pressure airflow are completely abandoned, and the accuracy of monitoring data is improved.

The application provides a method for constructing a Bmp4 gene knock-in mouse model, and the method comprises the following steps: obtaining sgRNA12 shown in SEQ ID NO. 3; mixing the sgRNA12, Cas9 mRNA and a targeting vector shown in SEQ ID NO. 1, and then microinjecting into a mouse zygote to obtain F0 generation mice; selecting F0 generation positive mice in genotype identification results of the F0 generation mice, and mating the F0 generation positive mice with wild type mice to obtain F1 generation mice with stable genotypes; and screening gene targeting mice with correct recombination, and mating the gene targeting mice with retinal specific knock-out tool mice Six3-Cre to obtain Bmp4 gene knock-in model mice. The method has high gene editing efficiency, and the transgenic mouse can highly express human Bmp4 protein.

The invention discloses an operation table for dissecting rats and mice, and relates to the technical field of mouse dissecting tables, the operation table comprises an experiment assembly and a laparotomy assembly, and the experiment assembly comprises a working plate; the laparotomy assembly comprises a rotating frame, a deformation plate and a clamping plate; the rotating frame is rotationally connected to the working plate through a friction hinge and is used for placing a mouse; during laparotomy and mouse observation, the rotating frame can drive the whole mouse to rotate, and the wound orientation is adjusted; a clamping plate is arranged at one end, far away from the rotating frame, of the deformation plate; the deformation plate is made of a bendable iron material, and the clamping plate is used for clamping and unfolding the epidermis of a mouse; according to the device, an operator can adjust the angle and the distance between the two clamping plates at will through the deformation plate capable of being manually bent, so that the device can be perfectly attached to mice of various body types from small size to large size, the problem that the belly is not fully lifted due to too loose clamping or the chest is pressed due to too tight clamping is solved, and a stable foundation is provided for anatomy operation.

The invention discloses a method for constructing a mammalian leucopenia model based on a Crispr gene editing technology. The invention belongs to the technical field of biology, and particularly relates to a method for constructing a mammalian leucopenia model based on a Crispr gene editing technology. The construction method of the leucopenia mouse model comprises the following steps: specifically mutating an H4K31 site in a mouse fertilized egg cell, so that the site is changed into arginine from lysine, and the leucopenia mouse model is obtained. A corresponding gene sequence is mutated at an H4K31 site in a fertilized egg cell of the specifically mutated mouse by means of cutting of a Crispr gene editing system and a fixed-point knock-in technology of homologous recombination. By detecting the immune function regulation and control mechanism of a homozygous mouse with the H4K31 site after the homozygous mouse with the H4K31 site is prepared, the mouse shows a natural leucopenia phenotype after lysine at the H4K31 site is mutated into arginine.

The invention discloses a construction method and application of a DNAJB6a gene specific knockout mouse model, and belongs to the technical field of gene engineering. Comprising the following steps: S1, designing gRNA sequences aiming at ninth to tenth exon regions of the mouse DNAJB6 gene; s2, in vitro transcription is carried out to prepare Cas9mRNA and gRNA; s3, carrying out microinjection on Cas9mRNA and gRNA (guide Ribonucleic Acid) into a fertilized egg of the mouse; s4, transplanting the fertilized ovum after injection into the body of a pseudo-pregnant female mouse to obtain an F0-generation mouse; s5, screening positive F0-generation mice through genotype identification, and mating the positive F0-generation mice with the wild type mice to obtain F1-generation heterozygote mice; s6, the F1-generation heterozygote mice are matched to obtain F2-generation homozygote mice, and the DNAJB6a gene specific knockout mouse model is established. The construction method for constructing the DNAJB6a gene knockout mouse model, provided by the invention, is simple and convenient to operate, short in construction process time and stable in genotype, and a DNAJB6a gene knockout mouse can be effectively obtained.

The invention discloses an ABPA animal model building method which comprises the following steps: building an ABPA animal model by injecting a live aspergillus fumigatus spore suspension into an airway, and selecting a mouse for adaptive feeding; a primary sensitization stage; repeating the excitation stage; a live aspergillus fumigatus spore colonization excitation stage; and establishing an evaluation model. The animal model which highly simulates the pathophysiological characteristics of human ABPA is constructed by performing sensitization and excitation in stages and in multiple modes, especially by introducing the key step of lung colonization of live aspergillus fumigatus spores; through increase of serum total IgE and aspergillus fumigatus specific IgE levels, airway eosinophilic granulocyte infiltration and high secretion of mucus, the evaluation model is successfully constructed, and a reliable experimental platform is provided for deep analysis of pathogenesis of diseases.

The invention discloses a construction method and application of a mouse model for testicular development disorder caused by Map3k1 gene mutation. The method comprises the following steps: designing and constructing sgRNA and a homologous recombinant vector capable of specifically recognizing a Map3k1 gene on the basis of a CRISPR / Cas9 system, injecting the sgRNA and the homologous recombinant vector into a mouse fertilized egg, screening out an F0-generation mouse with Map3k1 gene mutation from a mouse produced after the fertilized egg is transplanted, and hybridizing the F0-generation mouse with a wild type mouse to obtain a stably inherited Map3k1 gene mutation mouse model. The phenotype detection result of the mouse model shows that the fertility of the mouse model is remarkably reduced, and testis dysplasia conforms to the disease phenotype of testis dysplasia. The mouse model provides a convenient, reliable and economic means for researching the relationship between Map3k1 gene mutation and testicular dysplasia and the pathogenesis of the Map3k1 gene mutation and testicular dysplasia, and can be used for researching the pathogenesis of testicular dysplasia, a treatment method, drug screening and the like.

The application discloses a drunk animal model construction method and application thereof in drunk evaluation, and belongs to the technical field of model construction. The technical problems to be solved are that a traditional Kunming mouse drunk model is genetically hybrid, modeling is uneven, data repeatability is poor, drug efficacy verification is inaccurate, and there is no systematic drunk evaluation system. The technical solution points comprise providing a drunk animal model construction method for light and severe drunk animals and a light and severe drunk evaluation method. The drunk animal model construction method based on ICR mice is provided, the drunk model is reproducible, the evaluation indexes are quantifiable, and the drug screening is accurate, and the light and severe drunk evaluation method is provided.

The invention provides a method for constructing an emphysema mouse airway candida colonization model, and belongs to the technical field of candida colonization models.The method comprises the following steps that 1, an emphysema mouse model is constructed, including tobacco smoke exposure and elastase titration; and 2, establishing the pulmonary emphysema mouse Candida albicans airway colonization model, including preparation of a Candida albicans suspension and airway Candida colonization. The genomes of mice are closer to those of human beings than those of other animals, the mice have relatively wide gene, protein sequence and antibody availability, and the modeling cost of the mice is low, so that the mice are more suitable for experimental research of emphysema. Therefore, the construction method of the model is closer to a natural generation mode, is simple and convenient, can better simulate disease characteristics of candida albicans airway colonization in COPD, and creates conditions for further researching whether candida albicans airway colonization has a certain relationship with occurrence and development of COPD and acute exacerbation thereof.

The present application relates to a kind of CD69 humanization mouse model construction method, the construction method: (1) the targeting vector of expression humanization CD69 gene is constructed;(2) design and obtain sgRNA for mouse Cd69 gene;(3) the targeting vector, sgRNA and Cas9 protein are co-injected or co-electrotransferred to mouse fertilized egg cytoplasm or nucleus, and the fertilized egg is transplanted to pseudopregnant mouse, and genotype identification is carried out to pseudopregnant litter mouse, and the positive F0 mouse of correct human source fragment successfully inserted is screened;(4)F0 mouse is mated with background mouse to obtain F1 mouse, and CD69 humanization mouse model is screened out.The CD69 humanization mouse constructed in the present application has application value in immunology and other fields.

The invention discloses a high-efficiency C57BL / 6 mouse in-vitro fertilization method, relates to use of an in-vitro fertilization method in the technical field of biology, and particularly relates to a method for obtaining more high-quality fertilized eggs for gene editing in an in-vitro fertilization process of small animal gene editing model construction. Therefore, the animal cost of gene editing is greatly reduced, and wide application is promoted.

This invention relates to a mouse model of Parkinson's disease and its applications. The construction method includes the following steps: 1) in mice... Snack An exogenous fragment containing the human SNCA G51D coding sequence was knocked into the region upstream of the gene stop codon via homologous recombination, causing the human SNCA G51D coding sequence to interact with the mouse endogenous gene. Snack The gene forms a bicistronic expression structure, and the coding sequence of the human SNCA G51D is shown in SEQ ID No:1; 2) Positive F0 generation mice are backcrossed with wild-type mice, and genotypes are identified and screened to obtain a stable SNCA G51D endogenous knock-in Parkinson's disease mouse model. The gene editing strategy of the Parkinson's disease mouse model preserves the mouse's... Snack Simultaneous endogenous expression of α-synuclein G51D in the gene can mimic two pathogenic forms, exhibiting high physiological relevance and genetic authenticity.

This invention relates to a method for constructing a polycystic kidney disease (PCD) model and its applications. The method involves overexpressing the MYCN gene in a target animal to obtain a PCD model that leads to PCD-related phenotypes. The method includes the following steps: obtaining a first strain of mice with Rosa26 knock-in overexpressing the CAG-LSL-HA tag-MYCN-IRES-BFP-Wpre-polyA gene; obtaining a second strain of mice by inserting Cre-WPRE-polyA into the start codon of the Pax8 gene; and crossing the first and second strains of mice to obtain a MYCN-overexpressing PCD model. This invention employs various experimental methods for validation, including histopathological analysis, immunohistochemical staining, and Western blotting. The model provided by this invention overcomes the limitations of existing in vitro cell models, organoids, and existing animal models in terms of limited phenotypes. The established MYCN-overexpressing PCD animal model exhibits stable disease progression and a short disease cycle, which not only helps to elucidate the disease mechanism but also serves as an ideal platform for drug screening and efficacy evaluation.

The application discloses a special EVC cage for establishing an SPF level mouse closed colony animal model, which comprises an EVC cage body and a cage cover, a main body partition plate is inserted on the EVC cage body, and a partition plate clamping plate is elastically connected to the outer side wall of the EVC cage body, the main body partition plate is fixed on the EVC cage body through the partition plate clamping plate, a bedding trough is connected to the bottom of the EVC cage body, bedding trough side wall baffles are embedded on the front and rear side walls of the bedding trough, and a bedding trough partition plate which can be turned over is connected in the bedding trough. The main body partition plate can separate the EVC cage body, so that the influence of cage separation on pregnant female mice is reduced to the maximum, the possibility of postpartum eating of the female mice is reduced due to the shock of the pregnant female mice, and the bedding trough partition plate can separate the bedding with different female mouse odors, so as to prepare for the milk feeding of the baby mice.

This invention discloses a method for constructing a Brca2-p.Q462Ter genetically engineered mouse and its application. The Brca2 genetically engineered mouse provided by this invention carries a truncated mutation in its Brca2 gene. This mutation is located at exon 10 of the mouse Brca2-201 transcript, changing amino acid position 462 from glutamine Q (Gln) to the stop codon Ter, forming the Brca2-p.Q462Ter mutation. The Brca2-p.Q462Ter genetically engineered mouse is used to simulate the human BRCA2 gene truncated mutation p.Gln472Ter, c.1414C>T. Amino acid homology analysis shows that the mouse BRCA2 protein Q462 site is homologous to the human BRCA2 protein Q472 site; therefore, this mouse mutation site can be used to simulate the corresponding truncated mutation effect in human BRCA2. This genetically engineered mouse can simulate, at the whole animal level, the abnormal DNA damage repair, impaired homologous recombination repair function, decreased genome stability, and susceptibility to related diseases caused by BRCA2 truncated mutations, thus providing a reliable tool for research on BRCA2-related pathogenesis, developmental effects, drug screening, and efficacy evaluation.