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Method for extracting active ingredients from hibiscus sabdariffa calyx

A technology for roselle calyx and active ingredients, which is applied in the directions of medical preparations containing active ingredients, plant/algae/fungus/moss ingredients, pharmaceutical formulations, etc. The effect of high yield

Active Publication Date: 2012-12-12
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no domestic and foreign reports on the method of extracting active ingredients in roselle calyx with subcritical water, and no corresponding patent publications have been seen so far.

Method used

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  • Method for extracting active ingredients from hibiscus sabdariffa calyx

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Take 2.5000 g of roselle dried calyx powder, put it in the inner tank of the subcritical water extraction tank reactor, add 37.50 mL of water with a pH value of 1.0 at a liquid-solid ratio of 15:1 (v / w, mL / g), and extract The pressure was 5 MPa, the extraction temperature was 110 °C, and the extraction time was 20 min. After filtering, the filtrate was concentrated to dryness under reduced pressure, dissolved in water, and freeze-dried to obtain 1.1600 g of the active ingredient extract of Roselle calyx, with an extraction yield of 46.4%. , the anthocyanin content in the extract was 0.21 mg / g, and the polyphenol content was 13.0 mg / g.

[0023]

Embodiment 2

[0025] Take 2.5000 g of roselle calyx powder, put it in the inner tank of the subcritical water extraction tank reactor, add 50.00 mL of water with a pH value of 1.5 according to the liquid-solid ratio of 20:1 (v / w, mL / g), and extract the pressure. was 7 MPa, the extraction temperature was 130 °C, the extraction time was 40 min, filtered, the filtrate was concentrated to dryness under reduced pressure, dissolved in water and then freeze-dried to obtain 1.5850 g of the active ingredient extract of roselle calyx with an extraction yield of 63.4%. The anthocyanin content in the extract was 0.28 mg / g, and the polyphenol content was 13.3 mg / g.

[0026]

Embodiment 3

[0028] Take 2.5000 g of roselle dried calyx powder, put it in the inner tank of the subcritical water extraction tank reactor, add 62.50 mL of water with a pH value of 3.0 at a liquid-solid ratio of 25:1 (v / w, mL / g), and extract The pressure was 10 MPa, the extraction temperature was 150 °C, the extraction time was 50 min, filtered, the filtrate was concentrated to dryness under reduced pressure, dissolved in water, and freeze-dried to obtain 1.5750 g of roselle active ingredient extract, with an extraction yield of 63.0%. The content of anthocyanins in the extract was 0.13 mg / g, and the content of polyphenols was 13.3 mg / g.

[0029]

[0030] Determination of Antioxidant Activity of Roselle Active Component Extract

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Abstract

The present invention relates to a method for extracting active ingredients from hibiscus sabdariffa calyx, and belongs to the technical field of extraction of active ingredients from hibiscus sabdariffa. The extraction process comprises: crushing dried hibiscus sabdariffa calyx; filling the crushed calyx in a reaction kettle liner of a subcritical water extraction tank; adding water with a pH value of 1.0-3.0 according to a liquid-solid ratio of 15:1-25:1, wherein an extraction pressure is 5-10 MPa, an extraction temperature is 110-150 DEG C, and an extraction time is 20-50 min; filtering; carrying out reduced pressure concentration on the resulting filtrate to a dry state; adding water to dissolve; and carrying out freeze drying to obtain the active ingredient extract of the hibiscus sabdariffa calyx. Compared with the traditional water extraction method and the 30% ethanol extraction method, the method of the present invention has advantages of short extraction time, high extract yield, green environment protection, and no pollution.

Description

technical field [0001] The invention relates to a new method and process for extracting effective components in roselle calyx, in particular to a method and process for extracting effective components in roselle calyx with subcritical water. Background technique [0002] Roselle ( Hibiscus sabdariffa L) Also known as roselle, roselle, mountain eggplant, etc., Malvaceae hibiscus is an annual herb or perennial shrub, which is a traditional medicinal and edible plant. Roselle grows in tropical and subtropical regions. It is native to West Africa, India, and Malaysia. It has been planted for hundreds of years. It was introduced to Taiwan in 1910. Now it has grown widely in Guangdong, Guangxi, Fujian, Taiwan, and Yunnan. area planted. Traditional medicine believes that roselle calyx has the effects of clearing heat, quenching thirst and lowering blood pressure. Modern medical research has proved that roselle calyx has pharmacological effects such as anti-oxidation, anti-tumor, ...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P39/06A61K133/00
Inventor 仰榴青任月娜曲洪媛朱洋吴向阳赵婷冯伟伟郑伟
Owner JIANGSU UNIV
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