Method for extracting active ingredients from hibiscus sabdariffa calyx

A technology for roselle calyx and active ingredients, which is applied in the directions of medical preparations containing active ingredients, plant/algae/fungus/moss ingredients, pharmaceutical formulations, etc. The effect of high yield

Active Publication Date: 2012-12-12
JIANGSU UNIV
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no domestic and foreign reports on the method of extracting active ingredients in

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting active ingredients from hibiscus sabdariffa calyx

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0021] Example 1 :

[0022] Take 2.5000 g of roselle dried calyx powder, put it in the inner tank of the subcritical water extraction tank reactor, add 37.50 mL of water with a pH value of 1.0 at a liquid-solid ratio of 15:1 (v / w, mL / g), and extract The pressure was 5 MPa, the extraction temperature was 110 °C, and the extraction time was 20 min. After filtering, the filtrate was concentrated to dryness under reduced pressure, dissolved in water, and freeze-dried to obtain 1.1600 g of the active ingredient extract of Roselle calyx, with an extraction yield of 46.4%. , the anthocyanin content in the extract was 0.21 mg / g, and the polyphenol content was 13.0 mg / g.

[0023]

Example Embodiment

[0024] Example 2 :

[0025] Take 2.5000 g of roselle calyx powder, put it in the inner tank of the subcritical water extraction tank reactor, add 50.00 mL of water with a pH value of 1.5 according to the liquid-solid ratio of 20:1 (v / w, mL / g), and extract the pressure. was 7 MPa, the extraction temperature was 130 °C, the extraction time was 40 min, filtered, the filtrate was concentrated to dryness under reduced pressure, dissolved in water and then freeze-dried to obtain 1.5850 g of the active ingredient extract of roselle calyx with an extraction yield of 63.4%. The anthocyanin content in the extract was 0.28 mg / g, and the polyphenol content was 13.3 mg / g.

[0026]

Example Embodiment

[0027] Example 3 :

[0028] Take 2.5000 g of roselle dried calyx powder, put it in the inner tank of the subcritical water extraction tank reactor, add 62.50 mL of water with a pH value of 3.0 at a liquid-solid ratio of 25:1 (v / w, mL / g), and extract The pressure was 10 MPa, the extraction temperature was 150 °C, the extraction time was 50 min, filtered, the filtrate was concentrated to dryness under reduced pressure, dissolved in water, and freeze-dried to obtain 1.5750 g of roselle active ingredient extract, with an extraction yield of 63.0%. The content of anthocyanins in the extract was 0.13 mg / g, and the content of polyphenols was 13.3 mg / g.

[0029]

[0030] Determination of Antioxidant Activity of Roselle Active Component Extract

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention relates to a method for extracting active ingredients from hibiscus sabdariffa calyx, and belongs to the technical field of extraction of active ingredients from hibiscus sabdariffa. The extraction process comprises: crushing dried hibiscus sabdariffa calyx; filling the crushed calyx in a reaction kettle liner of a subcritical water extraction tank; adding water with a pH value of 1.0-3.0 according to a liquid-solid ratio of 15:1-25:1, wherein an extraction pressure is 5-10 MPa, an extraction temperature is 110-150 DEG C, and an extraction time is 20-50 min; filtering; carrying out reduced pressure concentration on the resulting filtrate to a dry state; adding water to dissolve; and carrying out freeze drying to obtain the active ingredient extract of the hibiscus sabdariffa calyx. Compared with the traditional water extraction method and the 30% ethanol extraction method, the method of the present invention has advantages of short extraction time, high extract yield, green environment protection, and no pollution.

Description

technical field [0001] The invention relates to a new method and process for extracting effective components in roselle calyx, in particular to a method and process for extracting effective components in roselle calyx with subcritical water. Background technique [0002] Roselle ( Hibiscus sabdariffa L) Also known as roselle, roselle, mountain eggplant, etc., Malvaceae hibiscus is an annual herb or perennial shrub, which is a traditional medicinal and edible plant. Roselle grows in tropical and subtropical regions. It is native to West Africa, India, and Malaysia. It has been planted for hundreds of years. It was introduced to Taiwan in 1910. Now it has grown widely in Guangdong, Guangxi, Fujian, Taiwan, and Yunnan. area planted. Traditional medicine believes that roselle calyx has the effects of clearing heat, quenching thirst and lowering blood pressure. Modern medical research has proved that roselle calyx has pharmacological effects such as anti-oxidation, anti-tumor, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K36/185A61P39/06A61K133/00
Inventor 仰榴青任月娜曲洪媛朱洋吴向阳赵婷冯伟伟郑伟
Owner JIANGSU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap