Method for extracting rutin from sophora japonica

A technology of sophora japonica and rutin, applied in the direction of fermentation and the like, can solve the problems of reduced yield, difficult treatment, environmental pollution, etc., and achieves the effects of low production cost, short extraction time, and no environmental pollution.

Inactive Publication Date: 2010-09-01
HENAN QINGCHUN BIOTECH
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Problems solved by technology

Rutin will be destroyed by high-temperature fumigation and hot water scalding, resulting in a decrease in yield, and the structure and precipitation of rutin will also be destroyed in the process of alkali dissolution and acid precipitation, and a large amount of acid water discharged wil

Method used

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Example Embodiment

[0015] The method for extracting rutin from Sophora japonica according to the present invention includes the following steps:

[0016] Step 1: Wash and dry the sophora rice in a fermentation tank, add 0.1-1% of rutin bioconvertase, and the total charge in the tank should be less than 70% of the tank capacity. After stirring evenly After 4-6 hours of closed fermentation, transfer to the extraction tank;

[0017] The second step: add a mixed solvent of methanol:ethanol=2:1 of 4.5 times the feed amount into the extraction tank, stir and extract for 30-40 minutes, filter to obtain the filtrate, and extract the filter residue repeatedly for at least three times;

[0018] Step 3: Combine the filtrates from the multiple extractions and place them in a distillation tower for distillation to recover the solvent to obtain a rutin extract;

[0019] The fourth step: Put the rutin extract obtained in the third step into the extraction tank, add 5 times the amount of the rutin extract to the mixed ...

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Abstract

The invention discloses a method for extracting rutin from sophora japonica, which comprises the following steps of: (1) cleaning and drying sophora japonica, putting the sophora japonica into a fermentation tank, adding rutin biotransformation enzymes for closed fermentation for 4-6 hours, and then, transferring the sophora japonica into an extraction tank; (2) adding 4.5 times of mixed solvent of methanol and ethanol into the extraction tank for stirring, extracting and filtering to obtain a filtered solution; (3) distilling the filtered solution, and then, recovering the solvent to obtain rutin extract; and (4) adding 3-5 times of quantity of mixed solvent of acetone, petroleum ether and n-octanol into the obtained rutin extract for conventionally stirring, extracting and filtering, distilling the filtered solution, and recovering the solvent to obtain high-purity rutin finished products. In the invention, the whole extracting process is closed and does not cause environment pollution; the extracting time is short, and the whole extracting and refining operation can be finished only in 16 hours; equipment used for extracting is conventional equipment, thus the enterprise burden can not be increased; and the solvent can be recycled, the production cost is low, the rutin yield is high, and the product purity meets the standard of State Pharmacopoeia and the standard of European Pharmacopoeia 6 (EP6).

Description

technical field [0001] The invention relates to a method for extracting rutin from Sophora japonica. Background technique [0002] Rutin is a glycoside synthesized by the dehydration of the hydroxyl group at the 3-position of Quercetin and Rutinose (a disaccharide composed of Glucose and Rhamnose). Rutin widely exists in the plant kingdom, and at least 70 kinds of plants containing rutin have been found, such as tobacco leaves, Sophora japonica, buckwheat and dandelion. In particular, Sophora japonica (the last flower bud of the plant Sophora japonica) and buckwheat have the highest content, which can be used as raw materials for extracting a large amount of rutin. Rutin is light yellow powder or very fine needle-like crystals, containing three molecules of crystal water, with a melting point of 174-178°C and anhydrous 188-190°C. Solubility: 1:10000 in cold water; 1:200 in hot water; 1:650 in cold ethanol; 1:60 in hot ethanol; 1:12 in cold pyridine. Slightly soluble in ac...

Claims

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Application Information

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IPC IPC(8): C12P19/60
Inventor 李松武
Owner HENAN QINGCHUN BIOTECH
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