33 results about "Rutinose" patented technology

The purpose of the present invention is to provide the following: a dried influenza vaccine preparation with which stable retention of the activity of influenza vaccine antigen is possible, even when the preparation is stored without strict low-temperature management, and with which a stable supply is possible; and a method for producing the dried influenza vaccine preparation. The present invention provides a dried influenza vaccine preparation comprising influenza vaccine antigen and a disaccharide, wherein the disaccharide is one or more selected from the group consisting of sucrose, maltose, palatinose, melibiose, isomalt, cellobiose, allolactose, isomaltose, sophorose, lactobionic acid, laminaribiose, xylobiose, turanose, gentiobiose, rutinose, kojibiose, nigerose, robinose, neohesperidose, sucralose, and maltitol.

The invention discloses isorhamnetin-3-O- β - The extraction and separation method of D-rutin, the dried gorse flower buds are crushed, refluxed, extracted, and concentrated to obtain a crude sample of the extract; the crude sample of the extract is first extracted and sectioned, and the solvent system is petroleum ether and ethyl acetate And n-butanol, the extracted part containing n-butanol is further separated and eluted by polyamide phenol resin column, the eluate is concentrated until it has no alcohol smell, the pH is adjusted to 2~3, cooled and stood, and filtered to obtain isorhamnetin-3 -O- β -D-rutinose; the isorhamnetin-3-O- that the inventive method makes β -D-rutinose content > 80%, the method has a high extraction rate, low equipment requirements, easy to expand production, and is suitable for industrial production.

The invention discloses rubus hirsutus anthocyanin and application thereof. The rubus hirsutus anthocyanin mainly comprises cyanidin-3-O-glucoside, pelargonine 3-O-glucoside and pelargonine 3-O-rutinoside, wherein the pelargonine 3-O-glucoside is main anthocyanin. An animal experiment proves that the rubus hirsutus anthocyanin can significantly reduce fasting blood glucose of db / db diabetic mice and shows excellent hypoglycemic activity in vivo. An in-vitro experiment proves that the rubus hirsutus anthocyanin can play a hypoglycemic role by inhibiting the alpha-glucosidase activity and promoting the glucose consumption of cells. Therefore, the rubus hirsutus anthocyanin can be taken as hypoglycemic functional food or a hypoglycemic drug for the prevention and the treatment of glycometabolism disorder-related diseases. The invention provides a new idea for comprehensive development and utilization of rubus hirsutus resources in China.

The invention provides a method for preparing kaempferol-3-O-rutinoside from a ginkgo leaf extract, which comprises the following steps: by using a two-dimensional liquid phase chromatography-mass spectroscopy combination technology and taking methanol-water or acetonitrile-water as a mobile phase and a reversed phase C18 chromatographic column as a one-dimensional preparative chromatographic column, performing component cutting on a ginkgo leaf extract, and collecting a target component which is a kaempferol-3-O-rutinoside crude product; and by taking a hydrophilic TECys as a two-dimensional preparative chromatographic column, performing component cutting on the kaempferol-3-O-rutinoside crude product, collecting, and concentrating through rotary evaporation to obtain the high-purity kaempferol-3-O-rutinoside, wherein the purity can be up to 80% or above. According to the invention, the preparation process is high in repetitiveness and favorable in operability; and meanwhile, ginkgo leaves are abundant in resources and easy to acquire. Thus, the invention meets the requirements for large-scale production and can be used for the preparation of raw materials for a Shuxuening injection.

The invention discloses a method for extracting myricetin-3-O-rutinose from taxus mairei leaves. The method comprises the steps of using a supercritical extraction kettle to extract, using methyl alcohol to absorb, using AB-8 resin to conduct chromatography, using 100-mesh polyamide to conduct the chromatography, collecting an eluent at 6-9min, concentrating and drying, and obtaining a white crystallized compound of myricetin-3-O-rutinose. The extracting method is simple and easy to implement, and the purity of myricetin-3-O-rutinose in the extracted white crystallized compound reaches 99.76%.

The invention relates to a method for extracting, separating and purifying four main anthocyanins from the blackcurrant residue. The object is to provide a simple, economic, green, eco-friendly, and high-yield method for extracting, separating and purifying four main high-purity anthocyanins from the blackcurrant residue. According to the method, the blackcurrant waste residue produced from industrial production is taken as a raw material, and homogenized pulverized coupling enzyme hydrolysis technology, negative-pressure cavitation reinforced extraction technology, macroporous adsorption resin concentration technology, medium-pressure quick-flash reverse chromatographic separation technology and low-temperature crystallization and recrystallization technology are adopted to obtain four anthocyanin monomer ingredients including delphinidin 3-O-glucoside, delphinidin 3-O-rutinoside, cyanidin 3-O-glucoside, and cyanidin 3-O-rutinoside and having the purity reach 95%. The method is abundant and available in raw materials, short in time consumption, less in solvent consumption, high in purity of obtained anthocyanins, low in production cost, and suitable for massive industrial production.