33 results about "Rutinose" patented technology

The invention relates to a method for extracting, separating and purifying four main anthocyanins from the blackcurrant residue. The object is to provide a simple, economic, green, eco-friendly, and high-yield method for extracting, separating and purifying four main high-purity anthocyanins from the blackcurrant residue. According to the method, the blackcurrant waste residue produced from industrial production is taken as a raw material, and homogenized pulverized coupling enzyme hydrolysis technology, negative-pressure cavitation reinforced extraction technology, macroporous adsorption resin concentration technology, medium-pressure quick-flash reverse chromatographic separation technology and low-temperature crystallization and recrystallization technology are adopted to obtain four anthocyanin monomer ingredients including delphinidin 3-O-glucoside, delphinidin 3-O-rutinoside, cyanidin 3-O-glucoside, and cyanidin 3-O-rutinoside and having the purity reach 95%. The method is abundant and available in raw materials, short in time consumption, less in solvent consumption, high in purity of obtained anthocyanins, low in production cost, and suitable for massive industrial production.

The invention provides a method for preparing kaempferol-3-O-rutinoside from a ginkgo leaf extract, which comprises the following steps: by using a two-dimensional liquid phase chromatography-mass spectroscopy combination technology and taking methanol-water or acetonitrile-water as a mobile phase and a reversed phase C18 chromatographic column as a one-dimensional preparative chromatographic column, performing component cutting on a ginkgo leaf extract, and collecting a target component which is a kaempferol-3-O-rutinoside crude product; and by taking a hydrophilic TECys as a two-dimensional preparative chromatographic column, performing component cutting on the kaempferol-3-O-rutinoside crude product, collecting, and concentrating through rotary evaporation to obtain the high-purity kaempferol-3-O-rutinoside, wherein the purity can be up to 80% or above. According to the invention, the preparation process is high in repetitiveness and favorable in operability; and meanwhile, ginkgo leaves are abundant in resources and easy to acquire. Thus, the invention meets the requirements for large-scale production and can be used for the preparation of raw materials for a Shuxuening injection.

The invention discloses a hudroxybutyl birutan derivant and preparing method, wherein the R1 is rutinose; R2,R3,R4,R5 is H or -CH, -C2H5, -CH2CH2OH, -CH2CH(OH)CH3, -CH2CH(OH)CH2CH3, -CH(CH3)CH(OH)CH3; at least one of R2, R3, R4, R5 is -CH2CH (OH) CH2CH3 or -CH (CH3) CH (OH) CH3. The preparing method comprises the following steps: mixing up with birutan or birutan derivant and epoxybutane to react; adopting one method of routine chromatogram, abstraction, hyperfiltration, ion exchange, electrodialysis, hyperfiltration to remove salt; drying to get yellow or light yellow powder hudroxybutyl birutan derivant.

The invention relates to a semi-synthesis method of luteolin and galuteolin as well as luteolin rutinoside by using hesperidin, a semi-synthesis method of the galuteolin by using hesperetin glucoside, and a semi-synthesis method of the luteolin by using hesperetin, and belongs to the field of chemistry and medicines. The semi-synthesis method comprises the following steps: enabling the hesperidin, the hesperetin glucoside and the hesperetin to be subjected to complexation in pyridine alcohol fluid, dehydrogenizing by using iodine, directly distilling alcohol and pyridine, maintaining for a period of time in an airtight distilled state, and carrying out demethylation reaction, so that the hesperidin is generated into the luteolin rutinoside; generating the galuteolin by demethylation of diosmetin glucoside, and generating the luteolin by diosmetin; and hydrolyzing the luteolin rutinoside, so that the luteolin rutinoside is transformed into luteolin and galuteolin. The semi-synthesis method has the advantages that two steps of dehydrogenation and demethylation are combined into one step, and reaction conditions of dehydrogenation and demethylation are mild and easy to control; few reagents are used and green and environmentally friendly; and the demethylation yield is high, and the industrial production is easy. Compared with disclosed documents and patients, the semi-synthesis method has great advantages in the production of luteolin and glucosides of the luteolin.

The invention discloses a prediction method for the content of raspberry kaempferol-3-O-rutinoside, and the method comprises the steps: building a prediction model through the temperature difference accumulated temperature and the dynamic changes of the index components of medicinal fruits, and achieving the dynamic prediction of the KR content based on the real-time accumulated temperature; and on the basis of the prediction model, secondary screening is carried out by referring to the fruit weight, so that the screening accuracy is improved. A raspberry medicinal fruit high-quality harvesting decision-making system is established through the prediction method, harvesting period prediction of a production place is given, it is guaranteed that the content of kaempferol-3-O-rutinoside reaches the standard, high quality and high price are achieved, benefits of enterprises and farmers are balanced, and technical support is provided for raspberry industry improvement.

The invention relates to antioxidative tartary buckwheat and oat liquor which is made from, by weight, tartary buckwheat, oat, sorghum, yeast for making hard liquor, rice chaff, pure water and biological tartary buckwheat flavone class substance. Each part of the biological tartary buckwheat flavone substance is composed of rutin, quercetin, kaempferol, kaempferol-3-rutinoside, quercetin-3-rutinoside, heteroside and isoquercetin. The making method includes the steps that firstly, tartary buckwheat and oat unblended liquor is brewed; then, biological tartary buckwheat and flavone substance is extracted by decocting tartary buckwheat grains and tartary buckwheat roots to be added back to the unblended liquor, and then fermentation and brewing are conducted; thirdly, 60-degree tartary buckwheat and oat unblended liquor is brewed; the unblended liquor obtained in the above steps is blended, filtered and then stored in a stainless steel tank for use; fourthly, laboratory test and measuring, blending or lowering the alcoholic strength and finished product packaging are conducted. The antioxidative tartary buckwheat and oat liquor has obvious functions of lowering blood glucose, lowering lipid, removing free radical and enhancing immunity and the like.

The invention discloses a detection method of gingko flavonol glycosides. The detection method comprises the following steps of: (1) preparing reference substances and a test solution, the reference substances being rutin, hyperoside, isoquercitrin, quercetin-3-O-2''-glucose rhamnoside, kaempferol-3-O-beta-D-rutinoside, narcissoside, syringetin-3-O-beta-D-rutinoside, cosmosiin, quercetin-3-O-p-coumaroyl rhamnose glucoside and kaempferol-3-O-p-coumaroyl rhamnose glucoside reference substances; (2) performing detection; (3) establishing a standard fingerprint spectrum; and (4) calculating the content of common peaks in the fingerprint spectrum established in the step (3). According to the detection method, a specific standard fingerprint spectrum of the ginkgo flavonol glycosides is established, the content of main flavonol glycosides in gingko and related extracts and pharmaceutical preparations thereof can be accurately detected, and the accuracy, precision, reproducibility and stability are good.

The invention discloses a method for preparing quercitrin and kaempferol-3-O-rutinoside from a Chinese redbud leaf extract, and belongs to the technical field of medicines. The method comprises the following steps: taking dried Chinese redbud leaves as raw materials, smashing the leaves, then extracting the leaves with 75% ethyl alcohol at the temperature of 50 DEG C, subjecting the extract to vacuum concentration, recovering the solvent, and obtaining a total extract; adding water to dissolve the total extract, and sequentially extracting with petroleum ether, dichloromethane and ethyl acetate to obtain different organic layers; carrying out vacuum concentration on the ethyl acetate layer, adding water into the obtained extract for dissolving, making the extract flow through AB-8 macroporous adsorption resin, and carrying out gradient elution by using ethanol with different concentrations to obtain corresponding elution parts; and carrying out vacuum concentration on the 60% ethanol elution part, dissolving with methanol, passing through a Sephadex LH-20 gel chromatographic column, and eluting with methanol to obtain a pure product of quercitrin and kaempferol-3-O-rutinoside. The extraction process is simple, a pure product of quercitrin and kaempferol-3-O-rutinoside is efficiently obtained, the used separation materials and solvents can be recycled, and the method is green, environmentally friendly, large in extraction amount and suitable for industrial production.

The invention discloses a method for extracting myricetin-3-O-rutinose from taxus mairei leaves. The method comprises the steps of using a supercritical extraction kettle to extract, using methyl alcohol to absorb, using AB-8 resin to conduct chromatography, using 100-mesh polyamide to conduct the chromatography, collecting an eluent at 6-9min, concentrating and drying, and obtaining a white crystallized compound of myricetin-3-O-rutinose. The extracting method is simple and easy to implement, and the purity of myricetin-3-O-rutinose in the extracted white crystallized compound reaches 99.76%.

The invention provides a method for determining the content of kaempferol-3-O-rutinoside in a radix tetrastigme medicinal material, and belongs to the technical field of chemical detection. Accordingto the method, the content of kaempferol-3-O-rutinoside in the radix tetrastigme medicinal material is determined through high performance liquid chromatography, the sampling amount is small, the dosage of an extraction solvent is small, an extraction solution does not need to be concentrated, the determination method is simple, convenient, rapid and good in repeatability, and the quality of the radix tetrastigme medicinal material can be accurately evaluated. The result of the embodiment shows that the separation degree of the kaempferol-3-O-rutinoside peak and other peaks in the spectrogramobtained by the method is greater than 1.5, which shows that the separation degree is good; in a linear relation test, the linear relation of the sample size of the determination method disclosed by the invention is good in a range of 0.004-0.12 [mu] g; in a repeatability test, the relative standard deviation of the content of kaempferol-3-O-rutinoside in the determination method is 2.76%, which indicates that the method provided by the invention has good repeatability.

The invention provides a capsule for treating infantile autism and a preparation method thereof. The capsule is prepared from the following raw materials in parts by weight: 33 to 62 parts of galactooligosaccharide, 12 to 28 parts of succinic anhydride, 20 to 33 parts of pumpkin polysaccharide, 22 to 40 parts of rutinose, 12 to 33 parts of rice fermentation filtrate, 12 to 25 parts of beta-carotene, 10 to 19 parts of strain, 10 to 20 parts of filler and 0.1 to 3 parts of adhesive. The raw materials are reasonably selected and scientifically proportioned, and all the components interact with one another to selectively stimulate the composition and activity of specific intestinal flora; and through a regulating system for regulating bidirectional signal communication between the intestinal tract and the nervous system, the composition and activity of the specific intestinal flora are selectively stimulated.

The invention relates to a preparation method of a lycium ruthenicum purified product, in particular to a method for simultaneously separating and preparing two petunidin anthocyanins in lycium ruthenicum. Purifying the crude extract by using macroporous resin, collecting acidic ethanol eluant, concentrating under reduced pressure to obtain anthocyanin macroporous resin eluant, further purifying the anthocyanin macroporous resin eluant by using a gel column, collecting the anthocyanin macroporous resin eluant in each 50mL/tube, collecting the fifth to seventh tubes and the 19th to 24th tubes, and respectively merging the eluant in sections to obtain the anthocyanin macroporous resin. Respectively carrying out vacuum concentration and freeze-drying on the two sections of eluents to obtain two petunidin anthocyanin, namely petunidin-3-O-rutinose (coumaroyl)-5-O-diglucoside and petunidin-3-O-rutinose (coumaroyl)-5-O-glucoside; the method is unique, two products with similar structures in the lycium ruthenicum can be separated at the same time, and an important theoretical basis is provided for qualitative and quantitative research of the anthocyanin of the lycium ruthenicum, structure-function relationship research of the anthocyanin of the lycium ruthenicum and standard substance development.