10 results about "Rutinose" patented technology

The invention provides a preparation method of a mixed molecularly imprinted polymer and a method for simultaneously extracting various flavonoid glycosides from a ginkgo biloba extract, and relates to the technical field of plant processing. According to the invention, rutin, isoquercitrin and apigenin-7-O-glucoside are used as template molecules to prepare a molecularly imprinted polymer (MIP); various characterizations prove that the preparation and synthesis are successful, and adsorption capacity tests prove that the compound has adsorption capacity on specific flavonoid glycoside. The MIP is added into the ginkgo flavone crude extract, and the total flavone content of the product after adsorption is increased from 24% to 90% or above. The four flavonoid glycoside compounds including rutin, isoquercitrin, 3-O rutinoside and apigenin-7-O-glucoside are obtained at a time, and it is indicated that the MIP has good adsorption performance, can be recycled and can be used for rapidly enriching the flavonoid glycoside compounds.

The invention relates to the technical field of coreopsis tinctoria refined components and preparation and application, and provides a coreopsis tinctoria refined component and a preparation method and application thereof.The coreopsis tinctoria refined component comprises, by mass, 11 specific components of phenolic acids, flavonol, flavanol and flavonoid, and the 11 specific components comprise, by mass, 2 parts of gallic acid, 1 part of potassium sorbate, 1 part of potassium sorbate, 1 part of potassium sorbate and 1 part of potassium sorbate. The health-care food is prepared from the following raw materials in parts by weight: 3 parts of chlorogenic acid, 14 parts of quercetin, 18 parts of catechin, 5 parts of citric acid, 2-3 parts of procyanidine B, 3 parts of (+)-epicatechin, 1-31 parts of 31 parts of procyanidine C, 14 parts of rutin, 4 parts of camphanol-3-O-rutinoside and 2 parts of isorhamnetin-3-O-rutinoside. The snow chrysanthemum is Changbai Mountain snow chrysanthemum. Animal experiments show that the refined component can significantly reduce the content of total cholesterol and triglyceride in serum of a high-fat model rat, the specific component with blood fat regulating activity is prepared from Changbai Mountain snow chrysanthemum, and a new choice is provided for non-drug intervention of dyslipidemia.

The invention discloses a genetically engineered bacterium for producing cyanidin-3-O-rutinoside as well as a construction method and application of the genetically engineered bacterium, and relates to the technical field of biological engineering. The invention provides an application of escherichia coli in production of cyanidin 3-O rutinoside. The modified recombinant escherichia coli can be used for efficient biosynthesis of cyanidin 3-O-rutinoside through whole-cell catalysis. Compared with plant extraction, the method has the advantages that the production conditions are mild, the product yield is increased, the specific synthesis of the product is realized, and an efficient and environment-friendly method with a wide development prospect is provided for the production and application of the cyanidin-3-O-rutinoside.

The present application belongs to the technical field of pigment preparation, and provides petunidin anthocyanins and blue pigments, and a preparation method and application thereof. The present application extracts anthocyanins from Lycium ruthenicum Murr by alcohol extraction and concentration; removes sugars, proteins and other impurities in the crude extract of Lycium ruthenicum Murr anthocyanins by using a macroporous resin; and then purifies the anthocyanin freeze-dried powder by using a preparative liquid chromatography system, and collects petunidin anthocyanins mainly composed of petunidin-3-O-(trans-p-coumaroyl) rutinoside-5-O-glucoside. The petunidin anthocyanins obtained by the present application are complexed with metal ions, and the blue pigments obtained have good color and stability. Compared with the prior art technical solution of adding polysaccharides to improve the stability of natural blue pigments, the petunidin anthocyanins of the present application can also ensure the stability of the blue pigments without adding polysaccharides, and at the same time, will not reduce the color value of the blue pigments.

The invention discloses a characteristic chromatogram for detecting a Yiyouqing particulate matter basis as well as a construction method and application thereof, and belongs to the field of quality control of traditional Chinese medicine compound preparations. According to the method, hydroxysafflor yellow A, rutin, kaempferol-3-O-rutinoside, astragalus smicus glycoside, flatstem milkvetch glycoside and kaempferol are taken as reference substances, multiple batches of samples of the Yiyouqing granules are analyzed by utilizing a high performance liquid chromatography-mass spectrometry technology, and a chemical component characteristic spectrum is established. Compared with a traditional method, the method has good precision and reproducibility, data are accurate and reliable, the six components can be recognized at the same time under the same chromatographic condition, analysis efficiency is greatly improved, synchronous monitoring of multiple components is achieved, and consistency and stability of preparation quality are guaranteed. The method provides support for improving the quality standard of the Yiyouqing granules, and has important significance on the clinical curative effect and medication safety of the Yiyouqing granules.

The present invention generally relates to compositions and methods for the treatment of cancer. More particularly, the present invention refers to compositions and methods for the treatment of hepatocellular carcinoma. Specifically, the present invention refers to a glycoconjugate useful for the treatment of hepatocellular carcinoma. Yet more specifically, the glycoconjugate is a rutinosylated coumarin derivative. This compound has proven herein to be efficient in experimental models of hepatocellular carcinoma both in vitro and in vivo.

The embodiment of the invention discloses construction of a plant multi-gene tandem vector and application of the plant multi-gene tandem vector to production of a flavone rutinoside compound in a plant chassis, relates to the technical field of plant genetic engineering, and aims at connecting multiple genes of flavone synthase FNS, methyltransferase OMT, glucosyltransferase GlcT and rhamnosyltransferase RhaT in series so as to obtain a plant expression plasmid; furthermore, flavonoid compounds such as diosmin and the like are produced in the plant land. The construction of the plant multi-gene tandem vector comprises the following steps: respectively carrying out double enzyme digestion on an intermediate vector pUC19-p35S-OMT-nos, an intermediate vector pUC19-OMT-GlcT2 and an intermediate vector pUC19-OMT-GlcT2-RhaT by using restriction endonucleases BsrGI and BamHI, recovering fragments, carrying out double enzyme digestion on pCAMBIA1300-FNSII by using restriction endonucleases Acc65I and BamHI, carrying out double enzyme digestion on pCAMBIA1300-FNSII by using restriction endonucleases BsrGI and BamHI, and recovering fragments by using restriction endonucleases BsrGI and BamHI. Connecting the recovered fragment with a linearized vector p1300-FNSII through T4 ligase, and carrying out sequencing verification to obtain a plant binary expression vector; the plant binary expression vector comprises the following components: pCAMBIA1300-FNSII-OMT, pCAMBIA1300-FNSII-OMT-GlcT2, and pCAMBIA1300-FNSII-OMT-GlcT2-RhaT, and the plant binary expression vector comprises the following components: pCAMBIA1300-FNSII-OMT-GlcT2, pCAMBIA1300-FNSII-OMT-GlcT2, and RhaT.

The application provides a method for increasing the content of effective components of rose by using edible fungus fermentation, and belongs to the technical field of biological fermentation.The method comprises the following steps: mixing rose powder, corn starch, glucose, potassium dihydrogen phosphate and water, adjusting the pH value, sterilizing, and obtaining a rose culture medium; inoculating the rose culture medium with a chrysophic acid fungus liquid, adding L-cysteine hydrochloride after 20-30h of fermentation culture, continuing the fermentation culture for 5-7d, and obtaining a rose fermentation product.The method can significantly increase the content of quercetin, quercetin-3-O-rutinose and polyphenol in the fermentation product by inoculating the rose with the chrysophic acid fungus for solid-state deep fermentation, has obvious tyrosinase inhibition capacity, is simple in process, low in production cost, and high in safety.The application further provides the rose fermentation product prepared by the method, the rose fermentation product, and the application of the rose fermentation product in the preparation of health care products.

The invention discloses a biosynthesis regulation and control method of kaempferol-3-O-rutinoside based on a phoebe bournei PbUGT92AE1 gene and application of the biosynthesis regulation and control method. According to the application, the phoebe bournei PbUGT92AE1 gene is successfully introduced into arabidopsis thaliana through an agrobacterium tumefaciens mediated inflorescence infection method, and a gene overexpression plant is obtained. Molecular detection results show that PbUGT92AE1 in a transgenic plant is remarkably and highly expressed, and the PbUGT92AE1 is remarkably different from a wild type. Furthermore, high performance liquid chromatography quantitative analysis is utilized to find that the content of kaempferol-3-O-rutinoside in the transgenic plant is obviously higher than that of the wild type. According to the application, the phoebe bournei PbUGT92AE1 gene is overexpressed in arabidopsis thaliana for the first time, so that the synthesis of kaempferol-3-O-rutinoside is effectively regulated and controlled, and the feasibility of improving the content of the secondary metabolite through a genetic engineering means is verified.

The application relates to the field of extraction of Radix Ternatae, and discloses a method for extracting kaempferol-3-O-rutinoside from Radix Ternatae, which comprises the following steps: (1) mixing the aboveground part and / or the underground part of Radix Ternatae and an ethanol-water solution to carry out reflux purification, and collecting the reflux extract; (2) sequentially extracting the reflux extract with n-hexane, ethyl acetate, chloroform and n-butanol, and obtaining n-hexane extract, ethyl acetate extract, chloroform extract and n-butanol extract after removing the solvents; and (3) separating the n-butanol extract through silica gel column chromatography and high-performance preparative liquid chromatography to obtain kaempferol-3-O-rutinoside. The method can effectively improve the extraction efficiency of kaempferol-3-O-rutinoside, and the obtained kaempferol-3-O-rutinoside has high purity.