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Detection method of gingko flavonol glycosides

A detection method and technology of flavonol glycosides, which are applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as the inability to guarantee the efficacy of preparations and the inability to use quality control to control the quality of finished products

Active Publication Date: 2021-04-23
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the current quality standard only controls the total flavonol glycoside content in Ginkgo biloba leaves, stipulating that its content should not be less than 24%, and does not make specific requirements on the specific flavonol glycoside content. Due to the characteristics of multiple targets, quality control with a single indicator cannot guarantee the efficacy of the preparation, and multi-component quality control can better control the product quality of Ginkgo biloba leaves
There is no good quality control method to accurately reflect the quality status of ginkgo biloba leaf extract, intermediates and finished products, so it cannot be used for quality control in the production process and quality control of finished products

Method used

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  • Detection method of gingko flavonol glycosides
  • Detection method of gingko flavonol glycosides
  • Detection method of gingko flavonol glycosides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1: the establishment of ginkgo biloba extract fingerprint

[0052] (1) Instruments and reagents

[0053] Shimadzu 2010A (HT) high-performance liquid chromatography, LC solution chromatography workstation, a total of 17 batches of Ginkgo biloba extract were provided by Yangzijiang Pharmaceutical Group Co., Ltd., and the information of the test products is shown in Table 1.

[0054] Table 1 Ginkgo biloba extract test product information

[0055]

[0056] (2) Chromatographic conditions

[0057] The chromatographic column is PRAZIS Absolute C18 chromatographic column (5μm, 4.6mm×250mm), the column temperature is 30°C, the detection wavelength is 360nm, the mobile phase A is 5% isopropanol-acetonitrile solution, and the mobile phase B is 0.4% phosphoric acid aqueous solution , the flow rate is 1.0mL·min -1 , analysis time 70min.

[0058] The gradient elution condition is 0-60min, mobile phase A: 16%→34%, mobile phase B: 84%→66%; 60-65min, mobile phase A: 34...

Embodiment 2

[0071] Embodiment 2: the establishment of Ginkgo biloba intermediate particle fingerprint

[0072] (1) Instruments and reagents

[0073] Shimadzu 2010A (HT) high performance liquid chromatography, LC solution chromatographic workstation, 15 batches of Ginkgo biloba intermediate granules were provided by Yangzijiang Pharmaceutical Group Co., Ltd., and the information of the test products is shown in Table 3.

[0074] Table 3 Ginkgo biloba intermediate granule test product information

[0075]

[0076]

[0077] (2) Chromatographic conditions

[0078] The chromatographic column is PRAZIS Absolute C18 chromatographic column (5μm, 4.6mm×250mm), the column temperature is 35°C, the detection wavelength is 350nm, the mobile phase A is 5% isopropanol-acetonitrile solution, and the mobile phase B is 0.4% phosphoric acid aqueous solution , the flow rate is 0.8mL·min -1 , analysis time 70min.

[0079] The gradient elution condition is 0-60min, mobile phase A: 16%→34%, mobile pha...

Embodiment 3

[0093] Embodiment 3: the establishment of fingerprint spectrum of ginkgo biloba intermediate voxel sheet

[0094] (1) Instruments and reagents

[0095] Shimadzu 2010A (HT) high-performance liquid chromatography, LC solution chromatography workstation, 13 batches of Ginkgo biloba intermediate tablets were provided by Yangzijiang Pharmaceutical Group Co., Ltd., and the sample information of the test product is shown in Table 5.

[0096] Table 5 Ginkgo biloba tablet test product sample information

[0097]

[0098] (2) Chromatographic conditions

[0099] The chromatographic column is PRAZIS Absolute C18 chromatographic column (5μm, 4.6mm×250mm), the column temperature is 30°C, the detection wavelength is 360nm, the mobile phase A is 7% isopropanol-acetonitrile solution, and the mobile phase B is 0.4% phosphoric acid aqueous solution , the flow rate is 1.0mL·min -1 , analysis time 70min.

[0100] The gradient elution condition is 0-70min, mobile phase A: 16%→34%, mobile pha...

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Abstract

The invention discloses a detection method of gingko flavonol glycosides. The detection method comprises the following steps of: (1) preparing reference substances and a test solution, the reference substances being rutin, hyperoside, isoquercitrin, quercetin-3-O-2''-glucose rhamnoside, kaempferol-3-O-beta-D-rutinoside, narcissoside, syringetin-3-O-beta-D-rutinoside, cosmosiin, quercetin-3-O-p-coumaroyl rhamnose glucoside and kaempferol-3-O-p-coumaroyl rhamnose glucoside reference substances; (2) performing detection; (3) establishing a standard fingerprint spectrum; and (4) calculating the content of common peaks in the fingerprint spectrum established in the step (3). According to the detection method, a specific standard fingerprint spectrum of the ginkgo flavonol glycosides is established, the content of main flavonol glycosides in gingko and related extracts and pharmaceutical preparations thereof can be accurately detected, and the accuracy, precision, reproducibility and stability are good.

Description

technical field [0001] The invention relates to a detection method of ginkgo flavonol glycosides, in particular to a detection method of ginkgo flavonol glycosides which can accurately detect the contents of main flavonoid glycosides in ginkgo and related extracts and pharmaceutical preparations. Background technique [0002] Ginkgo biloba is the dry leaf of Ginkgo biloba L. The cultivation area extends from Liaoning in the north, to Guangdong in the south, from Zhejiang in the east, to Shaanxi and Gansu in the west, and to Sichuan, Guizhou, and Yunnan in the southwest. It can be used for symptoms such as blood stasis blocking collaterals, chest pain and heartache. Its extract has various pharmacological effects such as improving cardiovascular and cerebrovascular circulation, protecting the heart, antagonizing PAF, and eliminating free radicals, and is often used to prevent and treat cardiovascular and cerebrovascular diseases. There are many pharmacologically active ingr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/86
CPCG01N30/02G01N30/06G01N30/14G01N30/8686G01N30/8679G01N2030/146
Inventor 刘利根杜丹阳张恩铚石心红杨琰薛铮刘嫣蓉刘莹周青梅
Owner CHINA PHARM UNIV
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