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Method for enriching beta-ethyoxyl rutinose

An ethoxylated rutose, enrichment technology, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc.

Inactive Publication Date: 2014-04-30
山西春阳生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the content of 1% is still not suitable for the production of hypoglycemic products, so it is necessary to further enrich β-ethoxyrutinose to lay the foundation for industrialization

Method used

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  • Method for enriching beta-ethyoxyl rutinose
  • Method for enriching beta-ethyoxyl rutinose

Examples

Experimental program
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Embodiment 1

[0012] Put 10 kg of tartary buckwheat raw materials with high β-ethoxyrutinose content through enzymatic conversion into a 200-liter extraction tank, add 100 liters of 70% ethanol, extract at room temperature for 2 hours, and extract the residue once more. Combine the ethanol extracts to obtain 196 liters of filtrate, concentrate to 20 liters, then add 20 liters of deionized water, then pass through the macroporous resin column AB-8 (20 × 180 cm) column, first elute with 10 liters of deionized water, and then Eluted with 10 liters of 5% ethanol, then eluted with 20 liters of 10% ethanol, and then eluted with 90% ethanol. The 10% ethanol eluate was collected and concentrated under reduced pressure to obtain a concentrate of about 2 liters. Put 2 liters of macroporous resin 10% ethanol elution concentrate on MCI column chromatography (5×150cm), elute with 5 liters of deionized water, 5 liters of 10% and 5 liters of 20% ethanol respectively, and use thin layer For detection by ch...

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Abstract

The invention discloses a method for enriching beta-ethyoxyl rutinose. The method specifically comprises the following steps that an enzymatic converted tartary buckwheat raw material with high beta-ethyoxyl rutinose content is extracted in ethanol, is depressurized to concentrate, is subjected to column chromatography by using a macroporous resin, and is further enriched by using an MCI column. The content of beta-ethyoxyl rutinose in a final extract is greater than 20%.

Description

technical field [0001] The invention relates to the technical field of food and health food production, in particular to a method for enriching β-ethoxyrutinose. Background technique [0002] Tartary buckwheat (Fagopyrum tataricum (Linn) Gaertn) is the seed of tartary buckwheat, a plant of the genus Buckwheat in the family Polygonaceae, which originated in China. [0003] The hypoglycemic active ingredient in tartary buckwheat has always been considered to be flavonoid (rutin). Some researchers have detected DCI from tartary buckwheat by HPLC, GC-MS and other methods, and speculate that the hypoglycemic activity of tartary buckwheat is mainly attributed to DCI. In our research, we found that the content of DCI in tartary buckwheat is not higher than that of sweet buckwheat, but the hypoglycemic activity of tartary buckwheat is better than that of sweet buckwheat. -rutinoside), has a good hypoglycemic effect. The natural content of β-ethoxyrutinose in tartary buckwheat is l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/04C07H1/06
Inventor 徐德平
Owner 山西春阳生物科技股份有限公司
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