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Effective part containing cyaniding 3-O-glucoside as well as preparation method and use thereof

A technology of glucoside and cyanidin, applied in medical preparations containing active ingredients, preparation of sugar derivatives, chemical instruments and methods, etc., can solve the problems of large side effects, high incidence of drug-induced diseases, etc., and achieve safety High performance and broad application prospects

Active Publication Date: 2008-08-27
天津市尖峰天然产物研究开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The side effects of western medicine treatment are large, some patients cannot tolerate them, and the incidence of drug-induced diseases caused by inappropriate western medicine treatment is higher

Method used

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  • Effective part containing cyaniding 3-O-glucoside as well as preparation method and use thereof
  • Effective part containing cyaniding 3-O-glucoside as well as preparation method and use thereof
  • Effective part containing cyaniding 3-O-glucoside as well as preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The effective fraction containing cyanidin 3-O-glucoside is prepared by the following method:

[0038] (1) Extraction: add 5000g of water to 500g of black soybean skin and extract by warm soaking at 50°C for 5 times. Serum;

[0039] (2) Macroporous resin adsorption separation: the extract in (1) is adsorbed by HP20 type macroporous resin, and water and volume percentage concentration are 10%, 30%, 50%, 70%, 95% ethanol aqueous solution gradient elution , each 250ml as a receiving volume, through thin-layer chromatography combined with analytical HPLC detection, collect components rich in cyanidin 3-O-glucoside, combine them, at a temperature ≤ 60 ° C, vacuum Concentrate under reduced pressure at 0.06-0.08 MPa to obtain a concentrate with a sugar content of 40, and the content of cyanidin 3-O-glucoside is 31.7%;

[0040] (3) Separation of polyamide resin: disperse the concentrate in water at a mass ratio of 1:4 for polyamide resin adsorption, and use water and ethanol ...

Embodiment 2

[0048] The effective fraction containing cyanidin 3-O-glucoside is prepared by the following method:

[0049] (1) Take black rice as raw material, add hydrochloric acid aqueous solution of 3 times of raw material quality as extraction solvent, the volume percentage concentration of hydrochloric acid aqueous solution is 1%, extract 6 times at 45 ℃, each time 8 hours, cross 80 mesh sieves, get extraction liquid;

[0050] (2) The extract is absorbed by the AB-8 type macroporous resin, and the concentration of water and volume percentage is 10%, 30%, 50%, 70%, 95% ethanol aqueous solution gradient elution, and the eluent passes through the thin layer Analytical method combined with analytical HPLC detection, collect components rich in cyanidin 3-O-glucoside, combine them, and concentrate under reduced pressure at a temperature ≤ 60 ° C and a vacuum degree of 0.06 ~ 0.08 MPa to obtain a sugar content of Concentrate of 10;

[0051] (3) Dispersing the concentrate in water at a mass...

Embodiment 3

[0053] The effective fraction containing cyanidin 3-O-glucoside is prepared by the following method:

[0054] (1) Take purple carrots as raw material, add sulfuric acid aqueous solution with 10 times the mass of raw material as extraction solvent, the volume percent concentration of sulfuric acid aqueous solution is 2%, extract once at 40°C for 4 hours, and pass through a 100-mesh sieve to obtain the extract ;

[0055] (2) The extract is absorbed by HPD100 type macroporous resin, and water and volume percentage concentration are 10%, 30%, 50%, 70%, 95% methanol aqueous solution gradient elution, and the eluent is passed through thin layer chromatography Combined with analytical HPLC detection, the components rich in cyanidin 3-O-glucoside were collected, combined, and concentrated under reduced pressure at a temperature of ≤60°C and a vacuum degree of 0.06-0.08MPa to obtain cyanidin 3-O-glucoside components with a sugar content of 40 concentrate;

[0056] (3) Dispersing the ...

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Abstract

The invention discloses an effective component containing cyanidin 3-O-glucoside, a preparation method and an application. The preparation method comprises (1) extraction: using black soybean hull as raw material, adding water, extracting at normal temperature, screening and obtaining extract, (2) absorbing the extract via a macroporous resin, eluting via water and alcohol water solution gradiently, checking the eluent, collecting the component rich in cyanidin 3-O-glucoside, depressurizing and concentrating to obtain concentrate, (3) absorbing the concentrate via polyamide resin, gradiently eluting via water and alcohol water solution, checking, collecting the component rich in cyanidin 3-O-glucoside, depressurizing and concentrating the eluent, and drying, to obtain the effective component whose cyanidin 3-O-glucoside content is higher than 50%. Tests prove that the effective component of cyanidin 3-O-glucoside is effective and non-toxic, with better analgesic, anti-acute inflammatory and anti-adjuvant rheumatoid arthritis functions.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract, in particular to an effective part containing cyanidin 3-O-glucoside, its preparation method and its application in the preparation of anti-rheumatoid arthritis medicine or food. Background technique [0002] Rheumatoid arthritis (rheumatoid arthritis, RA) is a chronic systemic autoimmune disease mainly characterized by joint lesions. " and so on. RA is characterized by recurrent attacks and high disability rate. It is a common and refractory disease. At present, there is no effective treatment at home and abroad[1]. Western medicine has many side effects, some patients cannot tolerate it, and the incidence of drug-induced diseases caused by inappropriate western medicine is higher. Traditional Chinese medicine not only has a wide range of sources, a large number of types, and small toxic and side effects, but also has the characteristics of multi-link, multi-level, and multi-target com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/065C07H1/08A61K31/7048A61P19/04A61P19/02A61P29/00
Inventor 刘岱琳刘丹於洪建
Owner 天津市尖峰天然产物研究开发有限公司
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