Vaccinium uliginosum cyanidin and separation and purification method thereof

Abstract

The invention relates to an anthocyanidin made from Vaccinium uliginosum L. and separation and purification methods thereof, which belong to the technology field of active component extraction and purification and functional food development. The content of anthocyanidin in Vaccinium uliginosum L. is 29.8-40%, wherein the content of malvidin accounts for 4.68-5.48% of the anthocyanidin; and antioxidant activity of the anthocyanidin is as 7.8-15 times as that of Vc. The preparation method includes pre-treating raw material, cold soaking and concentrating, purifying and concentrating, and freeze dying. The ultrasonic and microwave-assisted cold soaking method is employed for separation, and an AB-8 macroporous resin static-dynamic combined absorption purification method is employed. The invention provides an environmental friendly, high-efficiency and low-cost separation and purification technology of anthocyanidin made from Vaccinium uliginosum L., which maintains product bioactivity such as antioxidant property of product and ideal separation effect, establishes the basis for wide utilization of abundant resource of Vaccinium uliginosum L. and industrialized development promotion of a series of Vaccinium uliginosum L. health food, and embodies great economic benefit and social benefit in deep processing of the Vaccinium uliginosum L.

Description

technical field [0001] The invention belongs to the technical field of extraction and purification of functional active ingredients and development of functional foods, and relates to a method for separating and purifying anthocyanins from bilberry fruit. Background technique [0002] Vaccinium uliginosum L., also known as blueberry, Chinese blueberry, Dusi, Diguo, Dianguo, Dushi, etc., belongs to the genus Vaccinium L. of the family Ericaceae, and is a wild subshrub. plant. There are more than 130 species in the world, mainly distributed in China, Russia, Europe, North America, Japan, North Korea and other high-latitude countries in the northern hemisphere. Our country is mainly concentrated in Northeast my country, Inner Mongolia, Xinjiang and other regions. The wild Vaccinium vaccinium in Changbai Mountain area of ​​Jilin Province is widely distributed, with large yield and abundant resources. Bilberry contains flavonoids, polyphenols, polysaccharides and other functio...

AI Technical Summary

Problems solved by technology

[0011] (2) Bilberry anthocyanins are very sensitive to temperature, and heating for a long time can degrade and fade them

[0013] (4) Due to the natural anthocyanin products obtained by preliminary separation, the quality is usually low, the stability and colorability are not good, and further purification is required

[0014] (5) The market price of high-purity anthocyanin products is extremely expensive, because in the process of purifying anthocyanins, it is difficult to achieve the purpose of purifying them with only one method, and two or more methods are often required Coordination, such as initial separation by column chromatography, rapid detection by thin layer chromatography at the same time, and separation of purer anthocyanins by preparation of high-efficiency liquid phase, so its production process is extremely complicated, and there is no large-scale industrial production technology and equipment, restricting the application field and scope

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