The invention provides feed for culturing leeches and a preparation method of the feed. The feed for culturing the leeches is prepared from snail powder, freshwater mussel powder, blood powder, egg white, soybean meal, activin, rapeseed cakes, olive oil, potassium sulfate, potassium dihydrogen phosphate, sodium citrate, calcium lactate, calcium hydrophosphate, magnesium sulfate, potassium chloride, lentinan, chitosan, milkvetch root powder, radix salviae miltiorrhizae powder, garlic powder, sorbitol, compound microbial bacteria, complex vitamin, trace elements and an antioxidant. The feed is rich in nutritional ingredient, comprehensive in nutrient element, capable of effectively supplementing nutritional ingredients needed by leech growth, effectively promoting growth, shortening the leech growth cycle, reducing the culturing cost and increasing the economic benefit, free of pollution to a water body and friendly to the environment. The preparation method of the feed for culturing the leeches is simple and practicable in process, high in production efficiency, low in production and preparation cost, mild in preparation condition, easy to achieve, beneficial for market popularization and good in economic benefit.