Process of preparing high purity chlorogenic acid and flavonid with eucommia leaf

Abstract

The process of preparing high purity chlorogenic acid and flavonid with eucommia leaf includes the following steps: crushing eucommia leaf and extracting with pH 3-6 alcohol solution of 45 %-55 % concentration; saturation adsorbing the extracted solution with medium polarity JD-1 macroporous resin, and eluting successively with water, 20 %-45 % concentration alcohol solution and 60 %-75 % concentration alcohol solution to obtain crude chlorogenic acid product and flavonid-containing powder separately; eluting the crude chlorogenic acid product in silica gel column with ethyl acetate, acetone, methanol or ethanol, collecting the eluted solution, decompression concentrating, and re-crystallizing the concentrate in acetone or water to obtain refined chlorogenic acid product. The present invention has simple technological process, convenient operation, reuse of the macroporous resin, low comprehensive cost and excellent industrial application foreground.

Description

technical field [0001] The invention relates to a method for extracting and preparing active ingredients by using plants as raw materials, in particular to a method for preparing high-purity chlorogenic acid and total flavonoids from leaves of Eucommia ulmoides. Background technique [0002] Eucommia ulmoides is a traditional Chinese medicinal material with a history of more than 2,000 years in my country. Its functions are to nourish the liver and kidney, strengthen bones and muscles, and prevent miscarriage. At the same time, Eucommia ulmoides has special effects on treating hypertension. Chlorogenic acid and flavonoids are the main active components of Eucommia ulmoides leaves, which have significant physiological activities. Chlorogenic acid is a derivative of caffeoylquinic acid, which has multiple pharmacological effects such as choleretic, antibacterial, antiviral, antihypertensive, increasing white blood cells, and stimulating the central nervous system. It is an imp...

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Problems solved by technology

This process has the following disadvantages: the pretreatment of this process uses petroleum ether to degrease, the amount of petroleum ether is 2 to 3 times that of the raw material, and the solvent consumption is large, but generally it is impossible to achieve the desired effect in a short time, and at the same time it needs to add biological compound Enzyme impregnation for 24 to 36 hours, which makes the whole production cycle longer; at the same time, the process includes pretreatment, extraction, concentration, acid extraction, alkali neutralization, acid precipitation, recrystallization and other steps, and in some steps, biological Composite enzymes, reducing complex enzymes, etc., have many and cumbersome process steps, and involve many types of reagents and large quantities, which are not suitable for industrial production

This process is mainly completed by ultrasonic pretreatment and high temperature extraction, ultrafiltration, ethyl acetate extraction, D-140 resin separation and other steps. The patent mainly has the following shortcomings: (1) ultrasonic pretreatment before extraction, the process is cumbersome, The cost is high, and it is difficult to realize industrially; (2) chlorogenic acid is a heat-sensitive component, and high-temperature extraction is prone to decomposition. According to the extraction conditions of the CN1273964A patent, the recovery rate of chlorogenic acid is only 60% at 100 ° C for 80 to 120 min; (3) There are many steps in the patented process, and methods such as ultrafiltration, precipitant precipitation, and macroporous resin are used. The process is complicated, the equipment cost is high, the operation is cumbersome, and there are still problems such as unreasonable temperature selection during extraction. Therefore, it is difficult to realize industrial production