Process for preparing kaempferol derivatives

A technology of rutinoside and glucoside, applied in the field of medicine, can solve the problem of high production cost

Inactive Publication Date: 2007-10-03
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The raw material used in the existing preparation method is Hefei safflower whose appearance and properties of the medicinal material meet the requirements of the Pharmacopoeia (see the invention patent with application number 02137548.8 for details), but in the raw material, kaempferol-3-O-rutinoside, kaempferol-3-O- The content of glucoside is on the low side, and the total yield of both is no more than 0.5%, so the production cost is higher
According to the Pharmacopoeia, the safflower medicinal material "is the dried flower of Carthamus tinctorius L. of Compositae, which is picked when it changes from yellow to red in summer, and dried in the shade or in the sun" (see the 2000 edition of "The Pharmacopoeia of the People's Republic of China" for details). It can be seen that the flower color is The white or light yellow flowers of Compositae safflower have not been used as safflower medicinal materials. In fact, so far, there have been no reports of using them as raw materials to extract kaempferol derivatives

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0006] Example 1 Preparation of kaempferol derivatives kaempferol-3-O-glucoside and / or kaempferol-3-O-rutinoside from white flowers.

[0007] Take 1.8kg of dry white flowers of Compositae safflower, infiltrate them with 9L of 70% ethanol for 12 hours, then extract by percolation, collect 30 times the amount of percolation liquid, which is the extraction liquid; use a rotary thin film concentrator to recover the ethanol under reduced pressure After that, it is the sample solution on the column; the sample solution is passed into the chromatographic column equipped with macroporous resin (SIP-905 type, Shanghai Pharmaceutical Industry Research Institute) according to the sample volume of 0.3 times the column volume, and 8 times the amount is used successively. Water, 6 times the amount of 30% ethanol, and 10 times the amount of 95% ethanol were eluted; the 30% ethanol eluted part was collected; the 30% ethanol eluate was concentrated under reduced pressure until there was no alco...

Embodiment 2

[0012] Example 2 Prepare kaempferol-3-O-rutinoside and / or kaempferol-3-O-glucoside with light yellow flowers.

[0013] Take 2.0kg of dry light yellow flowers of Compositae safflower, heat and extract with 20L of 70% ethanol for 3 hours, filter, collect the filtrate, add 20L of 70% ethanol to heat and extract the remaining residue, filter, and collect the filtrate; combine the filtrates twice , is the extract; after the extract is decompressed and recovered with a rotary thin-film concentrator, it is the column sample solution, and the sample solution is passed into a polyamide powder (30-60 mesh, In the chromatographic column of Taizhou Luqiao Sijia Biochemical Plastic Factory), elution was carried out successively with 10 times the amount of water, 20 times the amount of 5% ethanol, 15 times the amount of 50% ethanol, and 10 times the amount of 95% ethanol. Collect 20 times of 5% ethanol eluate and 15 times of 50% ethanol eluate respectively, concentrate, and let stand for 12...

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PUM

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Abstract

The invention provides a novel process for preparing Kacmpferol derivative, i.e. Kacmpferol-3-0-rutinose glycoside and Kacmpferol-3-0-glucose glycoside monomer or their mixture by using the white flowers or light-yellow flowers of Carthamus tinctorius L. as raw material, extracting with water or water-containing ethanol, and isolating and purifying through microporous resin and silica gel column chromatography.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a new method for preparing kaempferol derivatives. The kaempferol derivatives referred to here refer to kaempferol-3-O-rutinoside and kaempferol-3-O-glucoside. Background technique [0002] Kaempferol derivatives kaempferol-3-O-rutinoside and kaempferol-3-O-glucoside are effective ingredients for preventing and treating cardiovascular and cerebrovascular diseases. According to reports, kaempferol derivatives also have analgesic effects, promote skin pigmentation, Antiallergic activity. The raw material used in the existing preparation method is Hefei safflower whose appearance and properties of the medicinal material meet the requirements of the Pharmacopoeia (see the invention patent with application number 02137548.8 for details), but in the raw material, kaempferol-3-O-rutinoside, kaempferol-3-O- The content of glucoside is on the low side, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C07H1/08
Inventor 郭美丽张戈
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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