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Application of pelargonidin 3-O-rutinoside in preparation of drug for resisting serratia marcescens infection

A technology of Serratia marcescens and rutinoside, which is applied in the field of medicine, can solve the problems that have not yet been reported on the anti-infection research report of fennelin-3-O-rutinoside, and achieve the clinical application prospect of prevention and treatment, inhibit Effect of Serratia marcescens infection, good clinical application prospect

Active Publication Date: 2020-11-27
XUZHOU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no research report on the anti-infection of fenugreek-3-O-rutinoside

Method used

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  • Application of pelargonidin 3-O-rutinoside in preparation of drug for resisting serratia marcescens infection
  • Application of pelargonidin 3-O-rutinoside in preparation of drug for resisting serratia marcescens infection
  • Application of pelargonidin 3-O-rutinoside in preparation of drug for resisting serratia marcescens infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Effects on the synthesis of signaling molecules regulated by quorum sensing:

[0022] Drugs: Anemonidin-3-O-rutinoside is obtained by separating and extracting the flower of the plant Pelargonium japonica. Those skilled in the art can directly purchase ananilin-3-O-rutinoside from Shanghai Yuanye Biological Co., Ltd.

[0023] Strain: Serratia marcescens NJ01 was purchased from China Medical Microbiology Culture Collection Center (CMCC, 41002).

[0024] Pick a single colony of Serratia marcescens NJ01 into LB medium, and culture it at 28°C and 180rpm for 17h. Take 100uL of the cultured bacterial solution and inoculate it into 100mL of fresh LB medium, and at the same time, add floridin-3-O-rutinoside at a final concentration of 50 and 100 μg / mL, and use DMSO as a control, and incubate at 28°C and 180rpm for 24h . The culture solution was centrifuged at 10000rpm at 4°C for 10min. The supernatant was extracted three times by adding an equal volume of acidified ethyl ace...

Embodiment 2

[0027] Inhibition of expression of quorum-sensing-regulated virulence factors:

[0028] A single colony of Serratia marcescens NJ01 was picked and inoculated into LB medium, and cultured at 28°C and 180rpm for 17h. Take 30uL of the cultured bacterial solution and inoculate it into 30mL of fresh LB medium, and at the same time, add floridin-3-O-rutinoside at a final concentration of 50 and 100 μg / mL, use DMSO as a negative control, and use 100 μg / mL of Vanillic acid (Vanillic acid, Van) was used as a positive control. Cultivate at 28°C and 180rpm for 24h. The culture solution was centrifuged at 10000rpm at 4°C for 10min.

[0029] Determination of protease activity: 100 mg of azo casein was dissolved in 5 mL of Tris / HCl with a concentration of 50 mM, and set aside. Take 125 μL of this solution and add it to 75 μL bacterial supernatant, mix well and incubate at 28° C. for 15 min, then add 600 μL of 10% trichloroacetic acid to terminate the reaction. After mixing, let stand at...

Embodiment 3

[0042] Inhibit the expression of quorum sensing-related genes:

[0043] Inoculate a single colony of Serratia marcescens NJ01 into LB medium, 28 ℃ , 180rpm culture 17h. Take 30 μL of the bacterial solution and add it to 30 mL of fresh LB medium, and mix well. Add 50 and 100 μg / mL of fennelin-3-O-rutinose, respectively, with DMSO as the control, 28 ℃ , 180rpm culture 24h. Bacteria in 4 ℃ , Centrifuge at 10000rpm for 10min. Remove the supernatant, wash the bacteria three times with pre-cooled PBS, centrifuge, and collect the bacteria. The total RNA was extracted using the RNeasy Mini Kit (Tiangen Bio, Beijing), and the RNA reverse transcription test was performed according to the method described in the RNeasy Mini Kit, setting fimA, fimC, flhD, bsmB, pigA, pigC and the internal reference gene rplU primers (shown in Table 1).

[0044] Table 1 Primers for the test

[0045] Table 1 Primers used in this study

[0046]

[0047] Applied Biosystems 7300 Real-time PCR Syste...

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Abstract

The invention discloses application of pelargonidin 3-O-rutinoside in preparation of a drug for resisting serratia marcescens infection. The invention discovers that the pelargonidin 3-O-rutinoside has the effect of inhibiting serratia marcescens infection for the first time, and does not inhibit the growth of serratia marcescens under an effective dose. The invention discovers that the combined medication of the pelargonidin 3-O-rutinoside and ciprofloxacin can effectively inhibit the formation of a serratia marcescens biofilm for the first time, and indicates that the pelargonidin 3-O-rutinoside has a good clinical application prospect in prevention and treatment of serratia marcescens infection.

Description

technical field [0001] The invention belongs to the medical technology, and in particular relates to the application of fenidin-3-O-rutinoside in the preparation of medicines for resisting Serratia marcescens infection. Background technique [0002] The overuse of antibiotics has led to the development of resistance in many pathogenic bacteria. The widespread use of antibiotics has brought selective pressure to bacteria. In order to resist the selective pressure brought by antibiotics, bacteria have gradually formed a defense system against antibiotics, that is, a layer of biofilm (Biofilm) is formed outside the cells. The biofilm wraps the cells inside and forms a natural barrier that can block most antibiotics outside the cells, making it difficult for antibiotics to penetrate the cell membrane into the cell interior, thus enhancing the resistance of bacteria to antibiotics. Therefore, changing the drug delivery strategy, discovering new targets, and developing drugs that...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P31/04
CPCA61K31/7048A61P31/04
Inventor 周金伟刘恩岐
Owner XUZHOU UNIV OF TECH
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