A kind of anthocyanin and use thereof
A technology of anthocyanins and fennel, which is applied in the field of medicine, and can solve problems such as the research and report on the hypoglycemic effect of anthocyanins in lentils.
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Embodiment 1
[0020] The preparation of embodiment 1 pomegranate anthocyanins
[0021] (1) Wash 1000g of fresh pomegranates, add 70% ethanol solution according to the ratio of material to liquid ratio 1:6 (w / v) and mix thoroughly, ultrasonically extract for 90min, vacuum pumping rate after ultrasonic, vacuum at 60°C Rotary evaporation removes ethanol, obtains the crude extract of the anthocyanin of the rotundum;
[0022] (2) Inject the crude extract of the pomegranate anthocyanins into the macroporous resin, rinse the resin with deionized water at a flow rate of 2BV / h, and then use 40%-80% ethanol solution with a flow rate gradient of 1BV / h Elution, collect the eluate with obvious red color, remove ethanol by vacuum rotary evaporation at 60°C, and freeze-dry to obtain the anthocyanin of fennel. According to calculation, 1000g of fresh fennel can be purified by the above steps to obtain 2.81g of flower color of fennel. Glycosides.
Embodiment 2
[0023] Example 2 Identification of the Anthocyanin Components of Pompomia
[0024] The pomegranate anthocyanins in Example 1 are made into a solution of 1 mg / mL, and detected by high performance liquid phase, the detection method is as follows: mobile phase is A phase (0.1% acetonitrile), B phase (1.5% formic acid aqueous solution), gradient Elution, 5%-60% Phase A elution for 30min, injection volume 10μL, temperature 30℃, flow rate 0.8mL / min, detection wavelength 520nm. At the same time, cyanidin-3-O-glucoside, geranidin-3-O-glucoside and geranidin-3-O-rutinoside standard substances were used for qualitative and quantitative analysis. figure 1 It is the high performance liquid chromatogram of the anthocyanin components of Pompanoderma, peak 1 is cyanidin-3-O-glucoside, peak 2 is geranidin-3-O-glucoside, peak 3 is geranidin-3-glucoside O-rutinoside, of which geranium-3-O-glucoside content accounts for 89.14% of the total anthocyanin content. The chemical structural formula o...
Embodiment 3
[0031] Example 3 The α-glucosidase inhibition experiment of fenugreek anthocyanins
[0032] Prepare a series of concentrations of Pentacarpus anthocyanin solutions, mix 5 μL of α-glucosidase solution (5U / ml, (dissolved in PBS, pH 6.8) and 5 μL of Pentacarpa anthocyanins, add 500 μL of PBS solution, and react at 37°C for 20 minutes. Add 10 μL of 10 mM pNPG (p-nitrophenyl-α-D-glucopyranoside) solution, react at 37 °C for 30 min, then add 500 μL of 1mol / L Na 2 CO 3 , the absorbance value is measured at 405nm, according to the formula (1-A sample 405 / A control405 ) to calculate the α-glucosidase half inhibitory concentration IC 50 . figure 2 and image 3 The results showed that the anthocyanins of Pompanoderma can effectively inhibit the activity of α-glucosidase, and its half inhibitory concentration IC 50 It was 0.23 μg / mL, and its activity was significantly stronger than that of the positive drug acarbose (33 μg / mL). Therefore, the anthocyanins of Pompomia can effectiv...
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