Method for extracting, separating and purifying four main anthocyanins from blackcurrant residue

An anthocyanin and pomace technology, applied in chemical instruments and methods, organic chemistry, preparation of sugar derivatives, etc., can solve the problems of lack of anthocyanin products, difficult to apply, cumbersome methods, etc., to shorten the experimental period and time-consuming. The effect of shortening and reducing production costs

Active Publication Date: 2017-11-07
GREATER KHINGAN RANGE CHAOYUE WILD BERRY DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the methods for separating and purifying anthocyanin monomers generally use semi-preparative high-performance liquid chromatography or high-speed countercurrent chromatography. These methods generally have shortcomings such as expe...

Method used

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  • Method for extracting, separating and purifying four main anthocyanins from blackcurrant residue
  • Method for extracting, separating and purifying four main anthocyanins from blackcurrant residue
  • Method for extracting, separating and purifying four main anthocyanins from blackcurrant residue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Add 1kg of blackcurrant pomace to 6L of water for homogenization and crushing three times, 5 minutes each time, adjust the pH of the slurry to 4 with citric acid, add 30g of cellulase, and then put the slurry in a constant temperature shaker for enzymatic hydrolysis at a temperature of 30°C 2h, filter with suction, and collect the filtrate and residue. In filter residue, add 12L ethanol and carry out negative pressure cavitation extraction, ethanol concentration is 80%, and negative pressure cavitation extraction pressure is-0.05MPa, extracts 3 times under room temperature, extraction time is 30min, the filtrate that obtains and step (1) The filtrates were combined, concentrated under reduced pressure and recovered to remove the solvent to obtain 168.6 g of blackcurrant extract. Select macroporous adsorption resin type AB-8 resin. After the above-mentioned blackcurrant extract was dispersed in 3L of pure water, the supernatant was centrifuged and loaded into a macropor...

Embodiment 2

[0038]Add 1kg of blackcurrant pomace to 8L of water to homogenize and break three times, each time for 5min, adjust the pH of the slurry to 5 with hydrochloric acid, add 50g of cellulase pectinase mixed enzyme, then put the slurry into a constant temperature shaker, Enzymolysis at 45°C for 4 hours, suction filtration, and collection of filtrate and residue. Add 15L ethanol to the filter residue and carry out negative pressure cavitation extraction, ethanol concentration is 80%, negative pressure cavitation extraction pressure is-0.07MPa, extract 4 times under room temperature, extraction time is 40min, the filtrate obtained and step (1) The filtrates were combined, concentrated under reduced pressure and recovered to remove the solvent to obtain 189.3 g of blackcurrant extract. Use macroporous adsorption resin model D101 resin. After the above-mentioned blackcurrant extract was dispersed in 2L of pure water, the supernatant was centrifuged and loaded into a macroporous adsorp...

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Abstract

The invention relates to a method for extracting, separating and purifying four main anthocyanins from the blackcurrant residue. The object is to provide a simple, economic, green, eco-friendly, and high-yield method for extracting, separating and purifying four main high-purity anthocyanins from the blackcurrant residue. According to the method, the blackcurrant waste residue produced from industrial production is taken as a raw material, and homogenized pulverized coupling enzyme hydrolysis technology, negative-pressure cavitation reinforced extraction technology, macroporous adsorption resin concentration technology, medium-pressure quick-flash reverse chromatographic separation technology and low-temperature crystallization and recrystallization technology are adopted to obtain four anthocyanin monomer ingredients including delphinidin 3-O-glucoside, delphinidin 3-O-rutinoside, cyanidin 3-O-glucoside, and cyanidin 3-O-rutinoside and having the purity reach 95%. The method is abundant and available in raw materials, short in time consumption, less in solvent consumption, high in purity of obtained anthocyanins, low in production cost, and suitable for massive industrial production.

Description

technical field [0001] The invention relates to a method for extracting, separating and purifying four high-purity anthocyanins from blackcurrant pomace, and belongs to the field of separation and purification of plant secondary metabolites. Background technique [0002] Black currant, the scientific name of black currant (Ribes nigrruu L.), commonly known as black bean fruit, alias black summer fruit, is a small deciduous shrub of the saxifrage family (saxifragaceae) and the genus Ribes, mainly produced in my country In Northeast China and northern Xinjiang, the fruit is small dark purple-brown berries, which can be eaten or processed into fruit juice, jam and other foods. Black currant is rich in sugar, organic acids, multivitamins, anthocyanins, active minerals and special aromatic components, and has high nutritional and medicinal value. Wherein the total anthocyanin content is 3.5mg / g fresh fruit weight (Zhang Zhidong 2002). The currently known curative functions of bl...

Claims

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Application Information

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IPC IPC(8): C07H17/065C07H1/08
CPCC07H1/08C07H17/065
Inventor 付玉杰焦骄黄玉岩顾成波李璐崔琦刘巨钊
Owner GREATER KHINGAN RANGE CHAOYUE WILD BERRY DEV
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