The invention relates to a method for preparing mono-glucoside through selective hydrolysis of flavone rutinoside or neohesperidoside, and belongs to the fields of chemistry and medicine. The method comprises the following steps: mixing flavone rutinoside or neohesperidoside with macroporous absorption resin in water or alkaline water solution, heating or adding acid; wherein in this process the diglucoside is dispersed, absorbed, and cured by the macroporous absorption resin; after drying, in the catalysis of acid or a dewatering agent, rhamnose in the molecule carries out condensation reactions with a ketone agent, and the rhamnose in the molecule is removed in the action of acid; eluting with an organic solvent or an alkaline water solution; and recycling the solvent or acidifying so as to obtain the flavone mono-glucoside. The method solves the problems that flavone diglucoside is hard to disperse in a ketone reagent and the selectivity of rhamnose hydrolysis is low. The method has the advantages of simple operation, easy recycling of organic solvent, low cost, high yield, and easiness in industrial production. Compounds such as hesperetin mono-glucoside, diosmetin mono-glucoside, hesperetin dihydrochlcone mono-glucoside, and the like, can be industrially produced from flavones such as flavone neohesperidoside through the method.