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Composition with blood sugar reduction function and application

A technology for lowering blood sugar and composition, applied in the field of compositions with hypoglycemic effect, can solve the problems of high dosage and limited hypoglycemic effect, and achieve the effects of reducing drug resistance and reducing dosage

Active Publication Date: 2020-07-10
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problem of limited hypoglycemic effect and high dosage of a single natural active ingredient in the prior art, the object of the present invention is to provide a composition with hypoglycemic activity, the active ingredients of which are hesperetin and geranium element; the composition has a synergistic effect to inhibit the activity of α-glucosidase, and has broad application prospects in the preparation of medicines for treating diabetes, health care products or food

Method used

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  • Composition with blood sugar reduction function and application
  • Composition with blood sugar reduction function and application
  • Composition with blood sugar reduction function and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A composition with hypoglycemic effect, the active ingredients of which are hesperetin and diosmin; the mass concentration ratio of hesperetin and diosmin in the composition is 3:10.

[0033] In vitro inhibition of α-glucosidase activity by hesperetin and diosmin at a mass concentration ratio of 3:10

[0034] 1.1 Reagents: α-glucosidase (α-glucosidase, Sigma, 750U), 4-nitrophenyl-α-D-glucopyranoside (pNPG, TOKYO Chemica Industry Co., LTD), acarbose (Acarbose , TOKYO ChemicaIndustry Co., LTD), corosolic acid, Na 2 HPO 4 , NaH 2 PO 4 .

[0035] Experimental equipment: microplate reader TECAN infinite M200 PRO (Teacan Group Ltd., Switzerland).

[0036] 1.2 Experimental steps:

[0037] 1.2.1 Preparation of drug solution: first prepare hesperetin, diosmin, acarbose and corosolic acid with dimethyl sulfoxide (DMSO) to form a 10 mg / mL mother solution, then use ultrapure water, Na 2 HPO 4 and NaH 2 PO 4Prepare 67 mmol / mL phosphate buffer solution (PBS, pH=6.8), prepar...

Embodiment 2

[0069] A composition with hypoglycemic effect, the active ingredients of which are hesperetin and diosmin; the mass concentration ratio of hesperetin and diosmin in the composition is 6:10.

[0070] In vitro inhibition of α-glucosidase activity by hesperetin and diosmin at a mass concentration ratio of 6:10

[0071] 2.1 Reagents: α-glucosidase (α-glucosidase, Sigma, 750U), 4-nitrophenyl-α-D-glucopyranoside (pNPG, TOKYO Chemica Industry Co., LTD), acarbose (Acarbose , TOKYO ChemicaIndustry Co., LTD), corosolic acid, Na 2 HPO 4 , NaH 2 PO 4 .

[0072] Experimental equipment: microplate reader TECAN infinite M200 PRO (Teacan Group Ltd., Switzerland).

[0073] 2.2 Experimental steps:

[0074] 2.2.1 Preparation of drug solution: first prepare hesperetin, diosmin, acarbose and corosolic acid with dimethyl sulfoxide (DMSO) to form a 10 mg / mL mother solution, then use ultrapure water, Na 2 HPO 4 and NaH 2 PO 4 Prepare 67 mmol / mL phosphate buffer solution (PBS, pH=6.8), prepa...

Embodiment 3

[0105] A composition with hypoglycemic effect, the active ingredients of which are hesperetin and diosmin; the mass concentration ratio of hesperetin and diosmin in the composition is 8:10.

[0106] In vitro inhibition of α-glucosidase activity by hesperetin and diosmin at a mass concentration ratio of 8:10

[0107] 3.1 Reagents: α-glucosidase (α-glucosidase, Sigma, 750U), 4-nitrophenyl-α-D-glucopyranoside (pNPG, TOKYO Chemica Industry Co., LTD), acarbose (Acarbose , TOKYO ChemicaIndustry Co., LTD), corosolic acid, Na 2 HPO 4 , NaH 2 PO 4 .

[0108] Experimental equipment: microplate reader TECAN infinite M200 PRO (Teacan Group Ltd., Switzerland).

[0109] 3.2 Experimental steps:

[0110] 3.2.1 Preparation of drug solution: first prepare hesperetin, diosmin, acarbose, and corosolic acid with dimethyl sulfoxide (DMSO) to form a 10 mg / mL mother solution, then use ultrapure water, Na 2 HPO 4 and NaH 2 PO 4 Prepare 67 mmol / mL phosphate buffer solution (PBS, pH=6.8), prep...

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Abstract

The invention discloses a composition with a blood sugar reduction function and application and belongs to the technical field of medicines. The composition with the blood sugar reduction function consists of hesperetin and diosmetin in a mass concentration ratio of (2:10)-(8-10), and preferably the mass concentration ratio of the hesperetin to the diosmetin is (3:10)-(8:10). The composition of the hesperetin and the diosmetin, which is disclosed by the invention, has a remarkable synergism in inhibiting alpha-glucosidase, has an effect prior to those of separately used flavonoids, in addition, is capable of reducing dosages of medicines and reducing drug resistance, and has wide application prospects in preparing medicines, healthcare products or foods for treating diabetes.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a composition with hypoglycemic effect and its application. Background technique [0002] With the development of industrialization, urbanization, population aging and changes in the ecological environment, lifestyle and dietary structure, diabetes has become one of the major chronic diseases that endanger human health around the world. Its salient features are high blood sugar levels and abnormal metabolism of lipids, proteins, and carbohydrates. It is clinically manifested as "three more and one less" symptoms such as polyphagia, polydipsia, polyuria, and weight loss. Studies have shown that α-glucosidase inhibitors can inhibit the activity of α-glucosidase on the brush border of small intestinal villous mucosa cells, and achieve the purpose of controlling postprandial hyperglycemia by blocking the digestion and absorption of carbohydrates. In addition, drugs that...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P3/10A23L33/105
CPCA23V2002/00A61K31/352A23L33/105A61P3/10A61K2300/00A23V2200/328A23V2250/2116
Inventor 张强焦中高刘杰超张春岭刘慧吕真真杨文博陈大磊
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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