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Method for separating and purifying three flavonoid glycosides from trichosanthes bark

A separation and purification, melon peel technology, applied in chemical instruments and methods, organic chemistry, sugar derivatives, etc., can solve the problems of few types of flavonoid glycosides, complicated separation process steps, human and environmental hazards, etc. Short cycle times, reagent savings, increased purity and efficiency

Active Publication Date: 2013-09-18
SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE +1
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Problems solved by technology

[0004] The above-mentioned method is mainly to systematically separate the chemical components of the n-butanol part of the skin of Trichosanthes L., and the types of flavonoid glycosides obtained are few, and the steps of the separation process are relatively cumbersome. Sometimes it is necessary to use chloroform as the extraction agent or eluent. There are serious hazards to the human body and the environment, and the production cycle is long and the production cost is high

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  • Method for separating and purifying three flavonoid glycosides from trichosanthes bark
  • Method for separating and purifying three flavonoid glycosides from trichosanthes bark
  • Method for separating and purifying three flavonoid glycosides from trichosanthes bark

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Embodiment

[0030] Example: The method for separating and purifying flavonoid glycosides from the traditional Chinese medicine Trichosanthes quince, the steps are:

[0031] (1) Preparation of the crude extract of Trichosanthes rind: After crushing the medicinal material of Trichosanthes piscifolia, heat and reflux extraction with 70% ethanol as solvent for 2 hours, count 4 times, combine the extracts, and concentrate under reduced pressure to obtain the crude extract.

[0032] (2) Extraction: Disperse the crude extract of Trichosanthes rind in water, extract with equal volumes of petroleum ether and ethyl acetate successively, concentrate the petroleum ether and ethyl acetate layers under reduced pressure respectively, and obtain the petroleum ether part and the ethyl acetate part .

[0033] (3) Coarse separation by polyamide column: disperse the above ethyl acetate in water, put it on a polyamide column for chromatography, first elute with water, then elute with ethanol-water solution ...

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Abstract

The invention relates to a method for separating and purifying three flavonoid glycosides from trichosanthes bark. According to the method, three high-purity flavonoid glycosides, namely quercetin-3-O-beta-rutinoside, apigenin-7-O-beta-glucoside and diosmetin-7-O-beta-glucoside, are obtained from the trichosanthes bark through the following steps of: (1) preparing a crude trichosanthes bark extract; (2) extracting; (3) carrying out crude separation by using a polyamide column; and (4) carrying out separation and purification by using semi-preparative high-performance liquid chromatography: carrying out separation and purification on total flavonoid glycosides of the trichosanthes bark by using the semi-preparative high-performance liquid chromatography, wherein the mobile phase is methanol-water. The method disclosed by the invention has the advantages that the process flow is environment-friendly, the damage to the environment is not serious, and the comprehensive cost is low.

Description

technical field [0001] The invention belongs to the field of chemical industry, and in particular relates to a method of separating and purifying three flavonoid glycosides (quercetin-3-O-β-rutinoside, apigenin-7-O-β-glucoside, and prime-7-O-β-glucoside) method. Background technique [0002] Trichosanthes peel is Cucurbitaceae Trichosanthes genus Trichosanthes ( Trichosanthes kirilowii Marim.) or Bilateral Trichiola ( Trichosanthes rosthornii Harms.), which is a commonly used traditional Chinese medicine, has the effects of nourishing the lungs and reducing phlegm, benefiting qi and widening the chest, etc. It is widely used clinically to treat cough with phlegm heat, phlegm turbid yellow thick, sore throat, chest pain, hematemesis, epistaxis, Constipation, carbuncle sore swelling and toxin embolism. Modern pharmacological experiments and clinical practice have shown that Trichosanthes peel has various pharmacological effects such as expanding coronary arteries, increas...

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Application Information

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IPC IPC(8): C07H17/07C07H1/08
Inventor 李爱峰柳仁民孙爱玲
Owner SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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