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Method for extracting and separating diosmetin from water chestnut peel

A technology of diosmin and water chestnut skin, which is applied in the direction of organic chemistry, can solve unseen problems, achieve low production cost, good separation effect, and meet the needs of the pharmaceutical industry

Inactive Publication Date: 2015-04-22
HEZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In our research, we found that diosminin is contained in water chestnut skin, but there is no literature report on the extraction and isolation of this compound from water chestnut skin

Method used

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  • Method for extracting and separating diosmetin from water chestnut peel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Add 8kg of fresh water chestnut skin powder into the extraction tank, add 32L of 70% by volume acetone aqueous solution, soak at 25°C for 24 hours, soak for 3 times in total, filter, combine the filtrates, concentrate under reduced pressure to obtain 1.05kg of extract .

[0024] The extract was added to 5.0 L of water to make a turbid liquid, extracted three times with 5.0 L of ethyl acetate, the extracts were combined and concentrated under reduced pressure to obtain 120 g of the extract.

[0025] Add 300mL methanol to the extract to dissolve, mix the sample with 260g polyamide, volatilize the methanol to dryness, install the column, connect to the MCI column for medium pressure separation, use 40-100% by volume methanol aqueous solution as the mobile phase gradient elution, Collect by 500mL each, detect by thin-layer chromatography, collect the eluate whose combined mobile phase concentration is 70-90% by volume, and concentrate under reduced pressure to obtain 31g of...

Embodiment 2

[0029] Add 8kg of fresh water chestnut skin powder into the extraction tank, add 32L of 70% by volume acetone aqueous solution, soak at 25°C for 24 hours, soak for 3 times in total, filter, combine the filtrates, concentrate under reduced pressure to obtain 1.05kg of extract .

[0030] The extract was added to 5.0 L of water to make a turbid liquid, extracted three times with 5.0 L of ethyl acetate, the extracts were combined and concentrated under reduced pressure to obtain 120 g of the extract.

[0031] Add 300mL methanol to the extract to dissolve, mix the sample with 260g polyamide, volatilize the methanol to dryness, install the column, connect to the MCI column for medium pressure separation, use 40-100% by volume methanol aqueous solution as the mobile phase gradient elution, Collect by 500mL each, detect by thin-layer chromatography, collect the eluate whose combined mobile phase concentration is 70-90% by volume, and concentrate under reduced pressure to obtain 31g of...

Embodiment 3

[0035] Add 8kg of fresh water chestnut skin powder into the extraction tank, add 32L of 70% by volume acetone aqueous solution, soak at 25°C for 24 hours, soak for 3 times in total, filter, combine the filtrates, concentrate under reduced pressure to obtain 1.05kg of extract .

[0036] The extract was added to 5.0 L of water to make a turbid liquid, extracted three times with 5.0 L of ethyl acetate, the extracts were combined and concentrated under reduced pressure to obtain 120 g of the extract.

[0037] Add 300mL methanol to the extract to dissolve, mix the sample with 260g polyamide, volatilize the methanol to dryness, install the column, connect to the MCI column for medium pressure separation, use 40-100% by volume methanol aqueous solution as the mobile phase gradient elution, Collect by 500mL each, detect by thin-layer chromatography, collect the eluate whose combined mobile phase concentration is 70-90% by volume, and concentrate under reduced pressure to obtain 31g of...

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Abstract

The invention discloses a method for extracting and separating diosmetin from a water chestnut peel and belongs to the field of natural organic chemistry. The method is characterized by comprising the following steps: extracting diosmetin from the water chestnut peel by taking an acetone water solution as an extraction solvent; extracting the extract by using ethyl acetate; performing chromatography separation on the extract by virtue of intermediate-pressure MCI and a polyamide column; repeatedly purifying by using Sephade x LH-20 gel chromatography to obtain a diosmetin product of which the content is larger than 95%. The method disclosed by the invention opens up a new way for extracting and separating the diosmetin from natural plants, and is good in separation effect, high in product purity and capable of changing the water chestnut peel which is always considered as waste into a valuable material; the used MCI resin, polyamide and gel can be reused; the raw materials are easily available; resources are sufficient; the production cost is low; large-scale production can be realized; the requirements of medical industry are satisfied.

Description

technical field [0001] The invention belongs to the field of natural organic chemistry, and relates to a method for extracting and separating diosmin, in particular to a method for extracting and separating diosmin from water chestnut skin. Background technique [0002] Diosmertin, whose chemical name is 4′-methoxy-5,7,3′-trihydroxyflavone, is a yellow needle-like crystal and is a natural flavonoid compound, mainly found in Labiatae plants In various plants such as mint, lemon, white thorn flower, Mongolian radish, velvet salvia, dandelion and so on. Modern studies have shown that diosmin has anti-oxidation, anti-infection, anti-shock, anti-mutagenic and anti-allergic properties and inhibits carcinogenesis. It can be used as an antiviral drug for the treatment of HIV and other viral infections, and can also treat various kind of inflammation. [0003] Derynodin can be isolated from plants, mainly through ethanol extraction, extraction, chromatography, chromatography, cryst...

Claims

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Application Information

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IPC IPC(8): C07D311/30C07D311/40
CPCC07D311/40C07D311/30
Inventor 刘珊罗杨合李行任宋慕波杨玉霞
Owner HEZHOU UNIV
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