Preparation method of corn silk flavonoid, and product and application thereof

A technology of flavonoids and corn, which is applied in the field of preparation of flavonoids from corn, can solve the problems of small added value in industrial utilization, low utilization rate, discarding and waste, etc., and achieve the effects of significant anti-inflammatory effect, fast dispersion and improved purity

Active Publication Date: 2016-04-20
JILIN INST OF CHEM TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a by-product of crops, corn silk has a wide range of sources and low cost, but most of them are discarded and wasted, the utilization rate is very low, and the added value of industrial utilization is very small. Appl...

Method used

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  • Preparation method of corn silk flavonoid, and product and application thereof
  • Preparation method of corn silk flavonoid, and product and application thereof
  • Preparation method of corn silk flavonoid, and product and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A preparation method of corn silk flavonoids, the preparation method comprising the extraction of corn silk flavonoids and the purification of corn silk flavonoids, the extraction method of the corn silk flavonoids is: selecting fresh corn silk without moths and mildew Raw materials, corn silk is washed, dried, crushed, passed through a 60-mesh sieve, and ethanol solution is used as an extraction solvent to extract corn silk flavonoids with microwave assistance. The ethanol concentration of the extraction solvent is 40-60%, and the extraction time is 15-25 minutes. The ratio is 1:25-1:45, the temperature is 60-80° C., and the extraction power is 400-500 W to obtain corn silk flavonoid extract, and dry to obtain corn silk flavonoid crude extract. Among them, microwave ultrasonic extraction instrument, model CW-2000, Shanghai Xintuo Analytical Instrument Technology Co., Ltd.; microwave frequency, 2450MHz; ultrasonic power / frequency: 50W / 40KHz; microwave action mode: non-pu...

Embodiment 2

[0067] On the basis of Example 1, the crude extract of corn silk flavonoids was obtained and then purified.

[0068] The purification method of the corn silk flavonoids is as follows: the corn silk flavonoids crude extract is made into a solution with a concentration of 1.0-1.5mg / mL, and the AB-8 macroporous adsorption resin is placed on the solution, the adsorption time is 2h, and 55-65% ethanol is used in 4BV Solution elution, the elution flow rate is 1.5 ~ 2mL / min, concentrated and dried to obtain the purified corn silk flavonoids.

[0069] Preferably, the crude extract of corn silk flavonoids is formulated into a solution with a concentration of 1.2 mg / mL, and is eluted with 58% ethanol solution, and the elution flow rate is 1.8 mL / min.

[0070] The purified corn silk flavonoids purified under the optimal purification conditions were identified, which contained apigenin, diosmin, luteolin, chlorogenic acid and rutin, the content ratio of apigenin and diosmin was 1:1, and l...

Embodiment 3

[0146] A corn silk flavone capsule, wherein the corn silk flavone can be the corn silk flavone crude extract in Example 1 or the corn silk flavone purified in Example 2. In this example, the corn silk flavone crude extract in Example 1 was used as a raw material to prepare capsules.

[0147] The content of corn silk flavonoid crude extract in each capsule is 0.5-0.6g / capsule, microcrystalline cellulose 56mg / capsule, calcium sulfate 47mg / capsule, magnesium stearate content 50-100mg / capsule, and the total weight of each capsule is about 0.6g.

[0148] Weigh corn silk flavonoids and various auxiliary materials according to the required quality, add and mix in equal amounts, use 80-90% ethanol liquid as a binder, wet granulate with a 16-mesh sieve, dry at 60°C for 30 minutes, granulate with a 16-mesh sieve and add Magnesium stearate is filled in No. 1 capsule according to the prescription amount.

[0149] Equal-volume incremental mixing is because powder mixing is not as easy as...

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Abstract

The invention discloses a preparation method of corn silk flavonoid. The preparation method comprises the following steps of extraction of the corn silk flavonoid and purification of the corn silk flavonoid, wherein the extraction method of the corn silk flavonoid comprises the steps: washing, drying, smashing and sieving corn silks; extracting the corn silk flavonoid by taking an ethanol solution as an extract solvent through microwave assisted extraction, wherein the ethanol concentration of the extract solvent is 40 to 60 percent, the extract time is 15 to 25 minutes, a material-to-liquid ratio is 1:45 to 1:25, the temperature is 60 to 80 DEG C, and the extract power is 400 to 500 W; obtaining a corn silk flavonoid crude extract by drying an extract solution; preparing the corn silk flavonoid crude extract into a solution of which the concentration is 1.0 to 1.5 mg/mL and using AB-8 macroporous resin to do the absorption, wherein the adsorption time is 2 hours; eluting by using 4BV of 55 to 65% ethanol solution, wherein the eluting flow rate is 1.5 to 2 mL/min; obtaining a purified corn silk flavonoid product through concentrating and drying, wherein the purified corn silk flavonoid product contains apigenin, diosmetin, luteolin, chlorogenic acid and rutin. The preparation method for extracting the corn silk flavonoid, disclosed by the invention, has the advantages of simpleness and efficiency, and the extracted corn silk flavonoid has a remarkable anti-inflammation effect and can be applied to development of anti-inflammation medicine and healthcare products.

Description

technical field [0001] The invention relates to the fields of natural product extraction and natural product medicine preparation, in particular to a preparation method of corn silk flavonoids and its product and application. Background technique [0002] Various Chinese herbal medicines have anti-inflammatory effects. Anti-inflammation of traditional Chinese medicine is an economical and affordable method with little side effects. However, there are some problems in this field. For example, the phenomenon of low-level repetition of traditional Chinese medicine in anti-inflammation is serious. Most of the clinical trials lack the theoretical basis of modern science. The short-term effect of the study is far more than the long-term effect. research etc. Therefore, it is not only necessary to screen effective drugs, but also to study pharmacology and chemical components of traditional Chinese medicine in depth. [0003] A large number of experimental studies and clinical in...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61K31/352A61K31/216A61K9/48A61P29/00
CPCA61K9/4866A61K31/216A61K31/352A61K31/7048A61K2300/00
Inventor 周鸿立张扬孙贺何罗香赵鹤鹏梁艳文翟婉辰
Owner JILIN INST OF CHEM TECH
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