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Method for separation and purification of luteolin, apigenin and diosmetin in trichosanthes peel

A technology for separation and purification of luteolin, applied in the direction of organic chemistry and the like, can solve the problems of cumbersome separation process steps, few types of flavonoids, harm to human body and environment, etc., and achieves short process cycle, reduced production cost, purity and efficiency. improved effect

Active Publication Date: 2013-10-09
SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE +2
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Problems solved by technology

[0004] The above-mentioned method is mainly to systematically separate the chemical components of the n-butanol part of Trichosanthes pipifolia, and the types of flavonoids obtained are few, and the steps of the separation process are relatively cumbersome. Sometimes it is necessary to use chloroform as the extraction agent or eluent, which is harmful to the human body. There are serious hazards to the environment and the environment, and the production cycle is longer and the production cost is higher

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  • Method for separation and purification of luteolin, apigenin and diosmetin in trichosanthes peel
  • Method for separation and purification of luteolin, apigenin and diosmetin in trichosanthes peel
  • Method for separation and purification of luteolin, apigenin and diosmetin in trichosanthes peel

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Embodiment

[0025] Example: The method for isolating and purifying luteolin, apigenin and diosmin from the peel of the traditional Chinese medicine Trichosanthes quinquefolium, the steps of which are as follows:

[0026] (1) Preparation of the crude extract of Trichosanthes barbatus: after crushing the medicinal material of Trichosanthes trichosanthes, heat and reflux extraction with 70% ethanol as solvent for 4 times, the extraction time is 2 hours, combine the extracts, and concentrate under reduced pressure to obtain the crude extract.

[0027] (2) Extraction: Disperse the crude extract of Trichosanthes rind in water, extract with equal volumes of petroleum ether and ethyl acetate successively, and concentrate the petroleum ether and ethyl acetate layers under reduced pressure respectively to obtain petroleum ether and ethyl acetate .

[0028] (3) Coarse separation by polyamide column: Disperse the ethyl acetate part of Trichosanthes pipifolia in water, put it on polyamide column for...

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Abstract

The invention relates to a method for separation and purification of luteolin, apigenin and diosmetin in trichosanthes peel. The method utilizes trichosanthes peel as a raw material and comprises the following steps of 1, preparing trichosanthes peel crude extract, 3, carrying out crude separation by a polyamide column, and 4, carrying out separation purification by semi-preparation high performance liquid chromatography. The method is green and environmentally friendly, does not produce damage on the environment and has a low comprehensive cost.

Description

technical field [0001] The invention belongs to the field of chemical industry, and in particular relates to a method for separating and purifying luteolin, apigenin and diosmin from the peel of the traditional Chinese medicine Trichosanthes quince. Background technique [0002] Trichosanthes peel is Cucurbitaceae Trichosanthes genus Trichosanthes ( Trichosanthes kirilowii Marim.) or Bilateral Trichiola ( Trichosanthes rosthornii Harms.), which is a commonly used traditional Chinese medicine, has the effects of nourishing the lungs and reducing phlegm, benefiting qi and widening the chest, etc. It is widely used clinically to treat cough with phlegm heat, phlegm turbid yellow thick, sore throat, chest pain, hematemesis, epistaxis, Constipation, carbuncle sore swelling and toxin embolism. Modern pharmacological experiments and clinical practice have shown that Trichosanthes peel has various pharmacological effects such as expanding coronary arteries, increasing coronary b...

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Application Information

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IPC IPC(8): C07D311/30
Inventor 李爱峰孙爱玲柳仁民
Owner SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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