Radical lobelia total flavones effective component and preparation thereof

A technology of effective components and total flavonoids, applied in the field of medicine, can solve problems such as large dosage

Inactive Publication Date: 2009-01-21
石任兵
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Lobelia is a commonly used traditional Chinese medicine in clinical practice, with significant curative effect and large dosage. However, there are no reports on the patents and related articles on the chemical components and preparation methods of Lobelia. We conducted a systematic study on it and found that it mainly contains flavonoids, and is Its main active ingredient

Method used

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  • Radical lobelia total flavones effective component and preparation thereof
  • Radical lobelia total flavones effective component and preparation thereof
  • Radical lobelia total flavones effective component and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: preparation technology of lobelia total flavonoids

[0037] Get Lobelia 1kg, 50% ethanol 10L reflux extraction 3 times, extract 1 hour each time, reclaim solvent under reduced pressure, obtain extract, add water to disperse, make the aqueous solution concentration be 3.84mg / mL (calculated by total flavonoids), take 3000 The speed of rotation per minute was centrifuged for 30min, and the supernatant was taken, and passed through 5LAB-8 macroporous adsorption resin, the adsorption flow rate was 1.0mL / min, the diameter-height ratio of the resin column was 1:8, and the sample loading was 7.68mg / mL ( Based on the amount of total flavonoids), 10% ethanol eluted 2 times the resin volume for impurity removal, and the impurity removal flow rate was 1.0mL / min, 70% ethanol eluted 5 times the resin volume, the elution flow rate was 2.0mL / min, and collected 70 % ethanol eluent, recover solvent, and dry under reduced pressure, which is the effective component of total f...

Embodiment 2

[0038] Embodiment 2: preparation technology of lobelia total flavonoids

[0039]Lobelia 1kg, 60% ethanol 15L, reflux extraction 3 times, extract 1.5 hours each time, reclaim solvent, extract adds water and disperses, makes the concentration of loading sample solution be 3.84mg / mL (in total flavonoid amount), makes the concentration of aqueous solution be 3.00mg / mL (based on the amount of total flavonoids), centrifuged at a speed of 3000 rpm for 30min, took the supernatant, and passed it through a 6L polyamide column (30-60 mesh), with an adsorption flow rate of 1.0mL / min. The ratio of diameter to height of the resin column is 1:7, the loading amount is 7.00 mg / mL (based on the amount of total flavonoids), and 10% ethanol is eluted to 2 times the resin volume for impurity removal. The flow rate of impurity removal is 1.0 mL / min, 50% 4 times the resin volume was eluted with ethanol, the elution flow rate was 2.0mL / min, the 50% ethanol eluate was collected, the solvent was recove...

Embodiment 3

[0040] Embodiment 3: preparation technology of lobelia total flavonoids

[0041] Lobelia 1kg, 70% ethanol 12L reflux extraction 2 times, extract 2 hours each time, reclaim solvent, add water to disperse the extract, make the concentration of the sample solution be 3.84mg / mL (in terms of total flavonoids), make the concentration of the aqueous solution 4.00 mg / mL (based on the amount of total flavonoids), centrifuge at a speed of 3000 rpm for 30 min, take the supernatant, and pass it through 7LAB-8 macroporous adsorption resin, the adsorption flow rate is 1.0 mL / min, and the diameter-height ratio of the resin column is 1:10, the sample loading is 8.00 mg / mL (based on the amount of total flavonoids), and water is eluted with 4 times the volume of the resin for impurity removal. The elution flow rate is 2.0mL / min, the 90% ethanol eluate is collected, the solvent is recovered, and dried under reduced pressure, which is the effective component of total flavonoids of Lobelia chinens...

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Abstract

The present invention discloses an effective component of total flavonoid extracted from Chinese medicine Chinese lobelia and a preparation method thereof. The preparation material mainly contains apigenin, luteolin, diosmetin, chrysin flavonoid, linarin, dafion, and indican compounds and other derivatives with the dafion as the mother nucleus. The present invention can be obtained by the methods of macroporous absorption resin, polyamide chromatography, solvent extraction and other methods.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a total flavonoid effective component of the traditional Chinese medicine Lobelia chinensis, a preparation method and its application in the fields of medicine and health food. Background technique [0002] Lobelia, also known as Jijiesuo and Hedyotis, is the rooted whole plant of the Campanulaceae plant Lobelia (Lobeliachinensis Lour.). It was first recorded in "Southern Yunnan Materia Medica", and later Li Shizhen also recorded it in "Compendium of Materia Medica". It mostly grows on the banks of paddy fields, beside ditches or in wet wastelands. Mainly produced in Anhui, Jiangsu, Sichuan, Zhejiang and other places. Lobelia is pungent and flat in nature and taste, and has the effects of diuresis, swelling, heat-clearing and detoxification. It is mainly used clinically to treat abdominal edema, facial and foot edema, carbuncle and furuncle, snake bites, late-stage...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/34A23L1/29A23L2/38A61K9/48A61K9/20A61K9/16A61K9/10A61K9/06A61K9/14A61P7/10A61P17/02A61P35/00A61P33/12A61K125/00A61K127/00A61K131/00A61K133/00A61K135/00A23L33/00
Inventor 石任兵姜艳艳刘斌戴莹王秋莹
Owner 石任兵
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