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Diosmetin having anti-inflammatory activity and application of diosmetin in preparation

A technology of geranialignin and activity, applied in the field of inflammation, can solve the problem of low anti-inflammatory activity of geranialignin

Inactive Publication Date: 2016-10-19
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In summary, there are relatively few reports on the anti-inflammatory activity of diosmin in the published inventions

Method used

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  • Diosmetin having anti-inflammatory activity and application of diosmetin in preparation
  • Diosmetin having anti-inflammatory activity and application of diosmetin in preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 Effect of diosmin on mouse foot swelling induced by complete Freund's adjuvant

[0015] Preparation of Kunming mouse animal model and experiment of diosmin on mouse paw swelling induced by complete Freund's adjuvant. 50 Kunming mice, half male and half female, body weight 18-22g, randomly divided into 5 groups: blank group, model group, celecoxib group (positive control), diosin low dose group, diosin high dose group groups, 10 mice in each group. According to the modeling method of Liu Mingpei et al., the blank group was intradermally injected with 0.1mL normal saline at the plantar part of the right hind foot of each mouse, the other groups were injected with complete Freund's adjuvant, and the inflammatory group was injected with the same dose on the 3rd and 6th days respectively. Freund's adjuvant was applied once, and when the toes, toes and even bare joints were red and swollen at the inflammatory site, the inflammatory model of mice was completed. The...

Embodiment 2

[0020] Example 2 Effect of diosmin on lipid mediators in serum of mice with adjuvant-induced paw swelling.

[0021] 2.1 Determination of cyclooxygenase 2 (COX-2)

[0022] According to mouse cyclooxygenase 2 (COX-2) enzyme-linked immunosorbent assay (ELISA) kit method, the content of COX-2 was determined. Take out the required strips from the aluminum foil bag after equilibrating at room temperature for 20 minutes, set the standard wells and sample wells, add 50ul of standard substances of different concentrations to each of the standard wells; first add 10ul of the sample to be tested in the sample well, and then add 10ul of sample diluent ; Blank wells are not added. In addition to the blank wells, add 100ul of HRP-labeled detection antibody to the standard wells and sample wells, seal the reaction wells with a sealing film, and incubate for 60min in a 37°C water bath or incubator. Discard the liquid, pat dry on absorbent paper, fill each well with washing solution, let it ...

Embodiment 3

[0033] Example 3 Effect of diosmin on free radicals in serum of mice with adjuvant-induced paw swelling

[0034] 3.1 Determination of total antioxidant capacity (T-AOC):

[0035] According to the instructions of the total anti-inflammatory oxidation capacity ELISA detection kit, the content of T-AOC was determined.

[0036]

[0037] Mix well, let it stand for 10min, measure the absorbance of each tube with a wavelength of 490nm, 1cm light beam, and double distilled water to zero.

[0038] Calculation formula:

[0039] Table 4 Effects of different drug groups on total antioxidant capacity (T-AOC) in serum of inflammatory mice

[0040]

[0041] Note: Compared with the model group, *P<0.05 has a significant difference, and **P<0.01 has a very significant difference.

[0042] It can be obtained from Table 4 that diosmin can enhance the total antioxidant capacity (T-AOC) in mice with inflammation.

[0043] 3.2 Determination of superoxide dismutase (sOD):

[0044] Accor...

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Abstract

The invention relates to a diosmetin having anti-inflammatory activity and application of the diosmetin in preparation of anti-inflammatory drugs. The diosmetin can remarkably inhibit inflammations caused by a free radical medium and a lipid medium and can be applied to the preparation of the anti-inflammatory drugs.

Description

technical field [0001] The invention relates to the field of inflammation, and relates to the anti-inflammatory activity of diosmin and its application in the preparation of anti-inflammatory drug dosage forms. Background technique [0002] Inflammation is one of the most serious health problems in the world. As people pay more and more attention to health, so people have more and more researches on inflammation. Diyerin is a natural flavonoid compound with anti-oxidation, anti-inflammation, anti-infection, anti-shock and other effects. Although there are many studies on the biological activity of diosmin similar molecular structure, such as diosmin compounds and their activity in the treatment of venous insufficiency are described in European patent specification EP0709383; Application in the preparation of drugs for the treatment of cardiovascular diseases; patent specification CN101132788A describes diosmin derivatives for the treatment and prevention of thrombotic disea...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61K9/48A61K9/16A61K9/20A61P29/00
Inventor 张保顺陈竹龚小保黄利挂
Owner SOUTHWEST UNIVERSITY
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