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Method for preparation of diosmetin from peanut shells

A technology of geranialignin and peanut shells, applied in the direction of organic chemistry, etc., can solve the problems of extracting geranialignin, reduce the cost of compound raw materials, etc., and achieve the effect of increasing the concentration and expanding the source.

Pending Publication Date: 2020-02-14
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the successful synthesis and separation of this compound have been reported many years ago, there is no detailed report on how to carry out cost control and can be separated from some wastes, such as the separation and purification of peanut shells. For example, the preparation in peanut shells will undoubtedly greatly reduce the raw material cost of the compound
However, in the literature, extracting and purifying flavonoids from peanut shells, mainly luteolin and eriodictyol, there is no report on extracting and purifying diosmin

Method used

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  • Method for preparation of diosmetin from peanut shells
  • Method for preparation of diosmetin from peanut shells
  • Method for preparation of diosmetin from peanut shells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Weigh 5g of peanut shell powder, extract at a material-to-liquid ratio of 1:15, add 75mL of 70% ethanol to it, extract at 80°C, heat in microwave for 10min, leave the filter residue after suction filtration and extract again under the same conditions, after the second extraction Combine the filtrates.

[0027] (2) Add 4 mL of 0.48 mol / L ferric chloride salt solution into the peanut shell extract obtained in step (1), carry out stirring reaction at room temperature, and then filter to obtain a clear filtrate. The reaction time is more than 1 hr.

[0028] (3) Preparative chromatographic conditions: the chromatographic column is a C18 chromatographic column (20 × 250mm, 5um), the mobile phase is methanol: water = 65:35, the flow rate is 12ml / min, the detection wavelength: 254nm, the column temperature: room temperature, and the injection volume : 2ml. Use preparative high performance liquid chromatography to separate the filtrate obtained in step (2), collect the chr...

Embodiment 2

[0030] (1) Weigh 5 g of peanut shell powder, extract with a material-to-liquid ratio of 1:10, add 50 mL of 70% ethanol to it, extract at a temperature of 80 ° C, and heat with microwave for 20 min. After suction filtration, the filter residue is extracted again under the same conditions. Combine the filtrates.

[0031] (2) Add 4 mL of 0.48 mol / L ferric chloride salt solution into the peanut shell extract obtained in step (1), carry out stirring reaction at room temperature, and then filter to obtain a clear filtrate. The reaction time is more than 1 hr.

[0032] (3) Preparative chromatographic conditions: the chromatographic column is a C18 chromatographic column (20 × 250mm, 5um), the mobile phase is methanol: water = 65:35, the flow rate is 12ml / min, the detection wavelength: 254nm, the column temperature: room temperature, and the injection volume : 2ml. Use preparative high performance liquid chromatography to separate the filtrate obtained in step (2), collect the chrom...

Embodiment 3

[0034] (1) Weigh 5 g of peanut shell powder, extract with a material-to-liquid ratio of 1:20, add 100 mL of 70% ethanol to it, extract at 80 ° C, and heat with microwave for 2 min. After suction filtration, the filter residue is extracted again under the same conditions. Combine the filtrates.

[0035] (2) Add 4 mL of 0.48 mol / L ferric chloride salt solution into the peanut shell extract obtained in step (1), carry out stirring reaction at room temperature, and then filter to obtain a clear filtrate. The reaction time is more than 1 hr.

[0036] (3) Preparative chromatographic conditions: the chromatographic column is a C18 chromatographic column (20 × 250mm, 5um), the mobile phase is methanol: water = 65:35, the flow rate is 12ml / min, the detection wavelength: 254nm, the column temperature: room temperature, and the injection volume : 2ml. Use preparative high performance liquid chromatography to separate the filtrate obtained in step (2), collect the chromatographic peaks ...

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Abstract

Belonging to the field of natural compound extraction, separation and purification, the invention provides a method for preparation of diosmetin from peanut shells. The method utilizes microwave-assisted extraction, employs an iron salt to remove luteolin and other components, and then performs purification by preparative high performance liquid chromatography to obtain diosmetin with purity of greater than 95%. The method is simple and rapid, and expands the sources of diosmetin.

Description

technical field [0001] The invention relates to a technology for preparing diosmin from peanut shells. The method adopts microwave-assisted extraction, and then purifies by preparative high-performance liquid chromatography to obtain diosmin. It belongs to the field of natural compound extraction, separation and purification. Background technique [0002] Diosmetin is a yellow powder, belonging to flavonoids, the English name is Diosmetin, the structure is shown below. [0003] [0004] According to reports, diosmin is a functional food, cosmetic and future drug with anti-oxidation, anti-infection and anti-shock effects, and it is also a natural flavonoid compound that inhibits the activity of human CYP1A enzyme. Dioxynignin has antimutagenic and antiallergic properties. Dioxynignin also has a strong effect of eliminating free radicals. It has also been reported that diosmin can reduce cell apoptosis caused by oxidative stress, and has the effect of protecting eyesight...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30C07D311/40
CPCC07D311/30C07D311/40
Inventor 孙立权刘莹
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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