9 results about "Eriodictyol" patented technology

The application relates to a method for bioconversion of naringenin to produce apigenin and dihydroquercetin, belonging to the field of biotechnology and food engineering, and comprising the following steps: (1) screening and mutagenic breeding of high-yield strains; (2) high-density fermentation for converting naringenin into apigenin and dihydroquercetin; and (3) extraction and refining to obtain high-purity apigenin and dihydroquercetin. Rhodococcus opacus OMK-104 bioconversion of cheap and easily available naringenin into high-value apigenin and dihydroquercetin has high conversion rate and high titer of apigenin and dihydroquercetin, and has a positive effect on reducing the production cost of apigenin and dihydroquercetin and promoting large-scale commercial application of apigenin and dihydroquercetin.

The invention belongs to the technical field of medicines, and discloses application of eriodictyol to prevention and treatment of cerebral apoplexy. Specifically, the invention discloses application of eriodictyol as a compound shown in a formula (I) in preparation of drugs for preventing and treating thrombotic stroke. Pharmacological experiments prove that after the eriodictyol is administered for 7 days, neurological function impairment, grasping ability impairment, muscular dystonia and cerebral ischemia volume increase caused by the thrombotic cerebral apoplexy can be obviously improved, which indicates that the eriodictyol has obvious pharmacological activity for preventing and treating the thrombotic cerebral apoplexy. Therefore, the eriodictyol can be used for preparing the medicine for preventing and treating the cerebral apoplexy, and has relatively high clinical application value and development prospect.

The present invention provides compositions comprising ruscogenin. The compositions of the present invention can be topically applied to the skin of a subject. The present invention further provides methods of applying compositions comprising ruscogenin to improve or treat a skin condition. The compositions of the present invention can also be applied to improve the overall appearance of the skin.

The embodiment of the invention discloses a drought stress method for Artemisia impatiens and application. The method comprises the following steps: selecting seeds of Artemisia impatiens with full grains and uniform size, disinfecting, sowing in a culture dish, placing in a culture box, and transferring germs into a plastic basin for sunlight greenhouse culture after germination; the seedlings are subjected to drought stress treatment under the conditions that the concentration of PEG-6000 ranges from 5% to 30%, and the time ranges from 2 d to 6 d. According to the method, Artemisia impatiens is selected as a research object, two drought stress conditions of soil water content and PEG-6000 solution are set, and the influence of drought stress on physiological and biochemical characteristics of Artemisia impatiens seedlings and the content of eriodictyol-7-methyl ether and isosakuranetin with anti-inflammatory and asthma-relieving activities is discussed. Furthermore, TGF-beta1 is used for inducing mouse airway smooth muscle cells to construct an asthma model, and it is verified that the artemisia differentiata can effectively improve the asthma relieving activity after being subjected to drought stress. The method provides a theoretical basis for high-quality and high-efficiency cultivation of Artemisia impatiens.

A method for producing eriodictyol and dihydroquercetin by biotransformation of naringenin belongs to the field of biotechnology and food engineering, and comprises the following steps: (1) screening and mutation breeding of high-yield strains; (2) converting naringenin into eriodictyol and dihydroquercetin through high-density fermentation; and (3) extracting and refining to obtain high-purity eriodictyol and dihydroquercetin. According to the method disclosed by the invention, the Rhodococcus opacus OMK-104 subjected to multiple rounds of ARTP mutation breeding is used for biologically converting the naringenin which is low in price and easy to obtain into the eriodictyol and the dihydroquercetin with high additional value, the conversion rate is high, the titers of the eriodictyol and the dihydroquercetin are high, and the method has a good application prospect in reducing the production cost of the eriodictyol and the dihydroquercetin. And the method plays a positive role in promoting large-scale commercial application of eriodictyol and dihydroquercetin.

The invention discloses a phyllostachys pubescens flavone synthase I gene PeFNSI1, an encoded protein and an application of the phyllostachys pubescens flavone synthase I gene PeFNSI1, belongs to the technical field of genetic engineering, and particularly relates to the phyllostachys pubescens flavone synthase I gene PeFNSI1, the nucleotide sequence of the phyllostachys pubescens flavone synthase I gene PeFNSI1 is shown as SEQ ID NO.1, and the application of the phyllostachys pubescens flavone synthase I gene PeFNSI1 in enhancing The invention reveals for the first time that flavone synthetase I (PeFNSI) coded by the phyllostachys pubescens PeFNSI1 gene can specifically catalyze flavone precursor naringenin to be converted into apigenin and catalyze eriodictyol to be converted into luteolin, and can activate a'bifunctional 'regulation element of a flavone synthesis and salicylic acid defense pathway at the same time by reprogramming salicylic acid metabolic flux, so that the flavone synthetase I (PeFNSI) can be used for preparing the apigenin and the luteolin. The multi-element synergistic effect of flavone synthesis, salicylic acid metabolic flux regulation and control and disease resistance enhancement is achieved, the limitation of an existing disease resistance strategy is broken through, and a brand new molecular tool is provided for plant disease resistance breeding.

The invention relates to the technical field of agricultural biology, in particular to a lipid-lowering eriodictyol-containing rubber seed three-stage isolate and application thereof. According to the method, an ultrasonic extraction technology is adopted, an extraction technology for optimizing total flavonoid compounds in natural rubber seeds is adopted, a rubber seed crude extract with high flavonoid substance content is obtained, three-stage isolates of the rubber seed extract containing eriodictyol active ingredients are separated through a multi-stage preparation technology, and the lipid-lowering effect of the rubber seed extract is verified on the cellular level.

The present invention relates to methods and transformed host cells for the production of eriodictyol from naringenin via bioconversion.

The invention discloses an application of a natural Tau-tubulin kinase 1 (TTBK1) inhibitor in anti-meningioma drugs, and belongs to the field of medicinal chemistry, the natural product is named eriodictyol-7-O-beta-D-glucuronic acid methyl ester, can form a novel and unique hydrogen bond with TTBK1, has a hydrophobic effect, can increase the ROS level in meningioma cells, and can be used for preparing anti-meningioma drugs. The action mechanism is unique, and structural design guidance is provided for TTBK1 inhibitor drug design. The compound can be used for preparing medicines for resisting malignant meningioma.