Extraction and assay method of total flavones of buchnera cruciata

A technology of determination method and extraction method, which is applied in the field of extraction and determination of total plant flavonoids, can solve problems such as inability to determine the medicinal mechanism of total flavonoids of black grass and hindering the medicinal value of black grass

Active Publication Date: 2016-12-07
GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Black grass has extremely high medicinal value. The content of total flavonoids in black grass and the content of ingredients in total flavonoids have not been disclosed in th

Method used

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  • Extraction and assay method of total flavones of buchnera cruciata
  • Extraction and assay method of total flavones of buchnera cruciata
  • Extraction and assay method of total flavones of buchnera cruciata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Pretreatment of D101 macroporous resin

[0042] Soak D101 macroporous resin with 95% ethanol for 24 hours, after fully swelling, remove the floating resin and sundries, pack the column with wet method, continue washing with 95% ethanol until the volume ratio of effluent and water is 1:3 If there is no white turbidity, wash with water until there is no alcohol smell, and set aside.

[0043] (2) Extraction and concentration of medicinal materials

[0044] Take 100g of black grass medicinal material, crush it into coarse powder, add 1000mL of ethanol with a volume concentration of 60%; heat and reflux for extraction 5 times, each time for 2 hours, filter the extract, and combine the filtrate; recover the ethanol from the filtrate, and concentrate until it has no alcohol smell.

[0045] (3) Put the concentrate in step (2) into the column of (1), wash with ethanol with a volume concentration of 10%, 30%, 50%, 70%, and 95% respectively until the color is lighter, collect...

Embodiment 2

[0048] (1) Instruments and reagents

[0049] ① Instruments: UV2550 UV-Vis spectrophotometer (Shimadzu, Japan), XS205 electronic balance (Mettler-Toledo, Switzerland)

[0050] ②Reagent: rutin reference substance (National Inspection Institute, 100080-200306), methanol, sodium nitrite, aluminum nitrate, sodium hydroxide

[0051] (2) Preparation of solution

[0052] ①Preparation of reference substance stock solution: Accurately weigh 10.37mg of rutin reference substance, add methanol to dissolve and dilute to the mark, and shake well to obtain (the concentration is 0.2074mg / mL).

[0053] ② Drawing of the standard curve: Precisely draw 0.0, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0mL of the reference substance stock solution into 25mL measuring bottles respectively, add water to 6.0mL respectively, and add 5% sodium nitrite solution by weight 1.0 mL, shake well, stand for 6 minutes, add 1.0 mL of 10% aluminum nitrate by weight, shake well, let stand for 6 minutes; add 10.0 mL of 4% sodium hy...

Embodiment 3

[0062] (1) Instruments and reagents

[0063] Instruments: High Performance Liquid Chromatography LC-10A (Shimadzu, Japan), Vemaron Chromatographic Workstation, XS205 Electronic Balance (Mettler-Toledo, Switzerland)

[0064] Test drugs: luteolin reference substance (Shanghai Yuanye Biotechnology Co., Ltd., LOT: YA0408YA13), apigenin reference substance (Shanghai Yuanye Biotechnology Co., Ltd., LOT: K18D5C1), diosmin reference substance (Shanghai Yuanye Biotechnology Co., Ltd. Technology Co., Ltd., batch number: PM0509RA13), methanol, acetonitrile, phosphoric acid.

[0065] (2) Preparation of solution

[0066] ①Preparation of reference substance solution: Precisely weigh 14.53mg of luteolin reference substance, 4.32mg of apigenin reference substance and 4.25mg of diosmin reference substance, put them in the same 50mL volumetric flask, dissolve and dilute to the mark with methanol, Shake well to obtain a reference substance stock solution with a luteolin concentration of 290.6 ...

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Abstract

The invention discloses an extraction and assay method of total flavones of buchnera cruciata. The total flavones of buchnera cruciata is extracted with a column filled with D101 macroporous resin in a manner of ethanol elution; with rutin as a reference substance, sodium nitrite, aluminum nitrate and sodium hydroxide are added to measure light absorbency to successively determine the content of the total flavones; the content of luteolin, apigenin and diosmetin in the total flavones of buchnera cruciata are detected through HPLC, wherein acetonitrile-0.1% phosphoric acid is a mobile phase, Agilent ZORBAX Eclipse XDB-C18 is a chromatographic column, and detection wave length is 350 nm. The total flavones of buchnera cruciata have the effects of anticoagulation, inflammation resistance, sedation and allergy resistance, and are low in toxicity. The extraction and assay method provides reference data for development on medicinal value of the total flavones of buchnera cruciata.

Description

technical field [0001] The invention relates to an extraction and determination method of total plant flavonoids, in particular to an extraction method and determination method of black grass total flavonoids extract. Background technique [0002] Many plants in nature contain flavonoids. It is estimated that 20% of traditional Chinese herbal medicines in my country contain flavonoids. Flavonoids have always been a hot research substance in the medicine and food industry, because flavonoids have various physiological effects, such as rutin, quercetin, and quercetin can enhance heart contraction and reduce the number of heart beats; Chrysanthemum, etc. have antitussive and expectorant effects; vitexin, wogonin, etc. have anti-tumor activity; silymarin has hepatoprotective effect, etc. The flavonoids contained in plants generally do not exist as a single component, but exist together in several components with similar or similar structures, and the total amount is called the...

Claims

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Application Information

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IPC IPC(8): G01N1/34G01N1/40G01N21/31G01N30/02
CPCG01N1/34G01N1/40G01N21/31G01N30/02
Inventor 黄建猷卢文杰陆国寿刘吉成黄周锋谭晓胡筱希
Owner GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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