Method for simultaneously separating and preparing two petunidin anthocyanins in lycium ruthenicum

A technology of petunidin and Lycium nigra can be used in the preparation of sugar derivatives, chemical instruments and methods, organic chemistry, etc., and can solve the problems of restricting further research and application of anthocyanins from Lycium nigra, and achieves convenient methods and easy handling. Large volume and good repeatability

Pending Publication Date: 2022-07-08
WOLFBERRY SCI INST NINGXIA ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research and development of reference substances that limit the commercialization of Lycium barbarum anthocyanins and their further research and application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: a method for simultaneously separating and preparing 2 petunidin-type anthocyanins in Lycium nigra, the method comprises the following steps:

[0017] (1) Extraction of anthocyanins: take Lycium ruthenicum black fruit as raw material, put it into a pulverizer after removing impurities, add liquid nitrogen to freeze it, quickly pulverize it to 80 meshes, extract with a 60% ethanol solution of pH=2 and then reduce the pressure Concentration gave crude extract.

[0018] (2) The above-mentioned concentrated crude extract was slowly injected into the D101 macroporous resin chromatography column, eluted with pH=2 water and pH=2 60% ethanol solution for 2 to 4 column volumes, and the acidic ethanol eluent was collected , and concentrated under reduced pressure at 40-50 °C to obtain anthocyanin macroporous resin eluent.

[0019] (3) The above-mentioned anthocyanin macroporous resin eluent is further purified by gel column, and the purification conditions are: the...

Embodiment 2

[0020] Embodiment 2: a method for simultaneously separating and preparing 2 petunidin-type anthocyanins in Lycium nigra, the method comprises the following steps:

[0021] (1) Extraction of anthocyanins: take Lycium ruthenicum black fruit as raw material, put it into a pulverizer after removing impurities, add liquid nitrogen to freeze it, quickly pulverize it to 90 meshes, extract with a 65% ethanol solution of pH=1.8 and then reduce the pressure Concentration gave crude extract.

[0022] (2) The above concentrated crude extract was slowly injected into the D4020 macroporous resin chromatography column, eluted with pH=1.8 water and pH=1.8 65% ethanol solution for 2 to 4 column volumes, and the acidic ethanol eluent was collected , and concentrated under reduced pressure at 40-50 °C to obtain anthocyanin macroporous resin eluent.

[0023] (3) The above-mentioned anthocyanin macroporous resin eluent is further purified by gel column, and the purification conditions are: the ge...

Embodiment 3

[0024] Embodiment 3: a method for simultaneously separating and preparing 2 petunidin-type anthocyanins in Lycium nigra, the method comprises the following steps:

[0025] (1) Extraction of anthocyanins: take Lycium ruthenicum black fruit as raw material, put it into a pulverizer after removing impurities, add liquid nitrogen to freeze it, quickly pulverize it to 100 meshes, extract with a 75% ethanol solution of pH=2.5 and then reduce the pressure Concentration gave crude extract.

[0026] (2) The above-mentioned concentrated crude extract was slowly injected into the DA201 macroporous resin chromatography column, eluted with pH=2.5 water and pH=2.5 75% ethanol solution for 2 to 4 column volumes, and the acidic ethanol eluent was collected , and concentrated under reduced pressure at 40-50 °C to obtain anthocyanin macroporous resin eluent.

[0027](3) The above-mentioned anthocyanin macroporous resin eluent is further purified by gel column, and the purification conditions a...

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Abstract

The invention relates to a preparation method of a lycium ruthenicum purified product, in particular to a method for simultaneously separating and preparing two petunidin anthocyanins in lycium ruthenicum. Purifying the crude extract by using macroporous resin, collecting acidic ethanol eluant, concentrating under reduced pressure to obtain anthocyanin macroporous resin eluant, further purifying the anthocyanin macroporous resin eluant by using a gel column, collecting the anthocyanin macroporous resin eluant in each 50mL/tube, collecting the fifth to seventh tubes and the 19th to 24th tubes, and respectively merging the eluant in sections to obtain the anthocyanin macroporous resin. Respectively carrying out vacuum concentration and freeze-drying on the two sections of eluents to obtain two petunidin anthocyanin, namely petunidin-3-O-rutinose (coumaroyl)-5-O-diglucoside and petunidin-3-O-rutinose (coumaroyl)-5-O-glucoside; the method is unique, two products with similar structures in the lycium ruthenicum can be separated at the same time, and an important theoretical basis is provided for qualitative and quantitative research of the anthocyanin of the lycium ruthenicum, structure-function relationship research of the anthocyanin of the lycium ruthenicum and standard substance development.

Description

technical field [0001] The invention relates to a preparation method of a purified product of Lycium nigra, in particular to a method for simultaneously separating and preparing two petunidin anthocyanins in Lycium nigra. Background technique [0002] Lycium ruthenicum Murr., belonging to the genus Lycium L. of Solanaceae (Solanceae), is a perennial, drought-resistant and salt-tolerant wild wolfberry resource discovered in recent years. It is mainly distributed in northwest China, in Europe and my country. There are also small distributions in sub-regions. Goji berry is rich in anthocyanin compounds, and its anthocyanin content accounts for 1.5%-3.2% of the dry fruit weight, much higher than other fruits and vegetables such as blueberries. [0003] Anthocyanins are one of the main active substances of Lycium nigra, with antioxidant, anti-inflammatory, inhibiting pancreatic lipase activity, improving immunity, lowering blood lipids, anti-tumor, improving memory and other effe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/065C07H1/08
CPCC07H17/065C07H1/08
Inventor 米佳闫亚美曹有龙禄璐罗青李晓莺戴国礼刘兰英何昕孺张曦燕安巍金波
Owner WOLFBERRY SCI INST NINGXIA ACAD OF AGRI & FORESTRY SCI
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