A method for extracting, separating and purifying four main anthocyanins from blackcurrant pomace

A technology for separation and purification of anthocyanins, applied in chemical instruments and methods, organic chemistry, sugar derivatives, etc., can solve the problems of lack of anthocyanin products, difficult to obtain application, high price, etc., to shorten the experimental period and time-consuming. , the effect of reducing production costs

Active Publication Date: 2020-04-28
GREATER KHINGAN RANGE CHAOYUE WILD BERRY DEV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, the methods for separating and purifying anthocyanin monomers generally use semi-preparative high-performance liquid chromatography or high-speed countercurrent chromatography. These methods generally have shortcomings such as expensive equipment and cumbersome methods, and cannot carry out large-scale separation and purification. It is difficult to be applied in actual production, which makes anthocyanin products lack in large quantities, and the price is very expensive

Method used

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  • A method for extracting, separating and purifying four main anthocyanins from blackcurrant pomace
  • A method for extracting, separating and purifying four main anthocyanins from blackcurrant pomace
  • A method for extracting, separating and purifying four main anthocyanins from blackcurrant pomace

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Experimental program
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Effect test

Embodiment 1

[0036] Add 1kg of blackcurrant pomace into 6L water for homogenization and crushing three times, 5min each time, adjust the pH of the slurry to 4 with citric acid, add 30g of cellulase, then put the slurry in a constant temperature shaker, and enzymatically hydrolyze at 30℃ 2h, suction filtration, collect the filtrate residue. Add 12L of ethanol to the filter residue for negative pressure cavitation extraction, the ethanol concentration is 80%, the negative pressure cavitation extraction pressure is -0.05MPa, and the extraction time is 3 times at room temperature. The extraction time is 30min. The obtained filtrate is followed by step (1) The filtrates in the mixture were combined and concentrated under reduced pressure to recover the solvent to obtain 168.6 g of blackcurrant extract. Use macroporous adsorption resin model AB-8 resin. After dispersing the blackcurrant extract in 3L of pure water, centrifuge to take the supernatant and load it into the macroporous adsorption re...

Embodiment 2

[0038] Add 1kg of blackcurrant pomace into 8L water for homogenization and crushing three times, 5min each time, adjust the pH of the slurry to 5 with hydrochloric acid, add 50g of cellulase and pectinase mixed enzyme, and then put the slurry in a constant temperature shaker. Enzymatic hydrolysis at 45°C for 4h, suction filtration, and collect the filtrate residue. Add 15L of ethanol to the filter residue for negative pressure cavitation extraction, the ethanol concentration is 80%, the negative pressure cavitation extraction pressure is -0.07MPa, and the extraction time is 4 times at room temperature, the extraction time is 40min, and the obtained filtrate is followed by step (1) The filtrates in the mixture were combined and concentrated under reduced pressure to recover the solvent to obtain 189.3 g of blackcurrant extract. Choose macroporous adsorption resin model D101 resin. After dispersing the blackcurrant extract in 2L of pure water, centrifuge to take the supernatant ...

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Abstract

The invention relates to a method for extracting, separating and purifying four main anthocyanins from the blackcurrant residue. The object is to provide a simple, economic, green, eco-friendly, and high-yield method for extracting, separating and purifying four main high-purity anthocyanins from the blackcurrant residue. According to the method, the blackcurrant waste residue produced from industrial production is taken as a raw material, and homogenized pulverized coupling enzyme hydrolysis technology, negative-pressure cavitation reinforced extraction technology, macroporous adsorption resin concentration technology, medium-pressure quick-flash reverse chromatographic separation technology and low-temperature crystallization and recrystallization technology are adopted to obtain four anthocyanin monomer ingredients including delphinidin 3-O-glucoside, delphinidin 3-O-rutinoside, cyanidin 3-O-glucoside, and cyanidin 3-O-rutinoside and having the purity reach 95%. The method is abundant and available in raw materials, short in time consumption, less in solvent consumption, high in purity of obtained anthocyanins, low in production cost, and suitable for massive industrial production.

Description

Technical field [0001] The invention relates to a method for extracting, separating and purifying four high-purity anthocyanins from blackcurrant pomace, and belongs to the field of separation and purification of plant secondary metabolites. Background technique [0002] Black currant, scientific name black currant (Ribes nigrruu L.), commonly known as black bean fruit, alias black summer fruit, is a small deciduous shrub of saxifraga family (saxifragaceae) and tea banana (Ribes), mainly produced in my country In Northeast China and northern Xinjiang, the fruits are small dark purple-brown berries, which can be eaten or processed into juice, jam and other foods. Blackcurrant is rich in sugar, organic acids, multiple vitamins, anthocyanins, active minerals and special aromatic ingredients, and has high nutritional value and medicinal value. The total anthocyanin content is 3.5 mg / g of fresh fruit weight (Zhang Zhidong 2002). The currently known therapeutic functions of blackcurra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/065C07H1/08
CPCC07H1/08C07H17/065
Inventor 付玉杰焦骄黄玉岩顾成波李璐崔琦刘巨钊
Owner GREATER KHINGAN RANGE CHAOYUE WILD BERRY DEV
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