Ginkgo leaf flavone extract as well as preparation method and characteristic spectrum construction method thereof

A feature map and extract technology, applied in the fields of biotechnology and medicine, can solve the problems of difficult quality control, unclear effect components of ginkgo biloba flavonoid extract, etc., and achieve the effect of good process stability

Active Publication Date: 2022-02-01
ZHEJIANG CONBA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] The present invention provides a high-purity flavonoid extract obtained from Ginkgo biloba extract (in line with national pharmacopoeia standards), the extract is named Ginkgo biloba flavonoid extract, And provide the preparation method of the flavonoid extract of ginkgo biloba and the construction method of the characteristic map, solve the problems of unknown effect components and difficult quality control of the flavonoid extract of ginkgo biloba

Method used

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  • Ginkgo leaf flavone extract as well as preparation method and characteristic spectrum construction method thereof
  • Ginkgo leaf flavone extract as well as preparation method and characteristic spectrum construction method thereof
  • Ginkgo leaf flavone extract as well as preparation method and characteristic spectrum construction method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1: Ginkgo biloba flavonoids preparation method-1

[0059] 1) Take 10 g of Ginkgo biloba extract that meets the national pharmacopoeia standard, add 8% (v / v) ethanol solution to prepare a 2% (v / v) sample solution;

[0060] 2) Pass the sample solution through a polyamide column (30-60 mesh) at a flow rate of 2BV / h, first wash it with 4 times the column volume (BV) of purified water at a flow rate of 2BV / h, discard the eluent, and then wash it with 7 times the column volume (BV) Volume 0.15% (v / v) sodium carbonate solution elution, flow rate 2BV / h, collect the sodium carbonate solution eluate;

[0061] 3) The sodium carbonate solution eluent is adjusted to pH 3 with hydrochloric acid to make a secondary sample loading solution;

[0062] 4) Pass the secondary sample solution through the polyamide column (the parameters are the same as above) at a flow rate of 2BV / h, first elute with 4 times the column volume of purified water at a flow rate of 4BV / h, discard the...

Embodiment 2

[0064] Embodiment 2: Ginkgo biloba flavonoid preparation method-2

[0065] 1) Take 10 g of Ginkgo biloba extract that meets the national pharmacopoeia standard, add 10% (v / v) ethanol solution to prepare a 2% (v / v) sample solution;

[0066] 2) Pass the sample solution through a polyamide column (10-30 mesh) at a flow rate of 1BV / h, first wash it with 3 times the column volume (BV) of purified water at a flow rate of 1BV / h, discard the eluent, and then wash it with 5 times the column volume (BV) Volume 0.5% (v / v) sodium carbonate solution elution, flow rate 1BV / h, collect the sodium carbonate solution eluate;

[0067] 3) The sodium carbonate solution eluent is adjusted to pH 5 with hydrochloric acid to make a secondary loading solution;

[0068]4) Pass the secondary sample solution through the polyamide column (the parameters are the same as above) at a flow rate of 1BV / h, first elute with 5 times the column volume of purified water at a flow rate of 3BV / h, discard the eluent, ...

Embodiment 3

[0070] Embodiment 3: Ginkgo biloba flavonoid preparation method-3

[0071] 1) Take 10 g of ginkgo leaf extract that meets the national pharmacopoeia standard, add boiling water (ie boiling purified water) to dissolve, and then add water to make a 2% (v / v) sample solution;

[0072] 2) Pass the sample solution through a polyamide column (80-100 mesh) at a flow rate of 3BV / h, first wash it with 5 times the column volume (BV) of purified water at a flow rate of 4BV / h, discard the eluent, and then wash it with 8 times the column volume (BV) Volume 0.1% (v / v) sodium carbonate solution elution, flow rate 4BV / h, collect the sodium carbonate solution eluate;

[0073] 3) Adjust the pH of the sodium carbonate solution eluent to 1 with hydrochloric acid to make a secondary loading solution;

[0074] 4) Pass the secondary sample solution through the polyamide column (the parameters are the same as above) at a flow rate of 3BV / h, first elute with 3 times the column volume of purified water...

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Abstract

The invention provides a ginkgo leaf flavone extract as well as a preparation method and a characteristic spectrum construction method thereof, the ginkgo leaf flavone extract comprises the following medicinal components: 3%-6% (w / w) of myricetin-3-O-rutinose, 3%-6% (w / w) of myricetin-3-O-glucose, 8%-11% (w / w) of rutin, 5%-7% (w / w) of tageted-3-O-rutinose, 5%-7% (w / w) of quercetin-3-O-rutinose, 6%-9% (w / w) of kaempferol-3-O-rutinose, 6%-10% (w / w) of isorhamnetin-3-O-rutinose, 13%-15% (w / w) of quercitrin-2''-(6''-p-cinnamyl glucosyl) and 11%-15%(w / w) of kaempferol-3-(6''-p-cinnamyl glucosyl-rhamnoside). The flavone extract is obtained by twice separation of a ginkgo leaf extract through a polyamide column, and can be used as an antioxidant in food, medicines and health care products. According to the method, the problem that the effect components of the ginkgo leaf flavone extract are unknown is solved, the quality control difficulty is reduced, and the established ultra-high performance liquid characteristic chromatogram has specificity for quality detection.

Description

technical field [0001] The invention relates to the fields of biotechnology and medical technology, in particular to a ginkgo leaf flavonoid extract, a preparation method thereof, and a characteristic map construction method. Background technique [0002] Ginkgo biloba extract is a natural herbal medicine widely used for cardiovascular and cerebrovascular diseases. Its main components are flavonoids, lactones, organic acids, etc., among which flavonoids are one of the main active substances and index substances. A small part of the flavonoid structure in Ginkgo biloba is aglycone, which includes quercetin, kaempferol, isorhamnetin, myricetin, luteolin, apigenin, cloveting, dihydrokaempferol, etc.; most of them are the above-mentioned glycosides The 3- or 7-position glycosides of the element are mainly monosaccharides, disaccharides, and trisaccharides such as D-glucose, L-rhamnose, and rutinose. In addition, flavone glycosides containing cinnamoyl groups such as quercetin-2...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/16A61P39/06G01N30/88A61K127/00
CPCA61K36/16A61P39/06G01N30/88A61K2236/331A61K2236/333A61K2236/55G01N2030/884
Inventor 何厚洪姚建标丁楠平夏婷王建方郑亚纯朱小翠
Owner ZHEJIANG CONBA PHARMA
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