Extraction and Separation of Rhamnetin-3-o-β-d-Rutinose from Gorse Flower Buds

Abstract

The invention discloses isorhamnetin-3-O- β - The extraction and separation method of D-rutin, the dried gorse flower buds are crushed, refluxed, extracted, and concentrated to obtain a crude sample of the extract; the crude sample of the extract is first extracted and sectioned, and the solvent system is petroleum ether and ethyl acetate And n-butanol, the extracted part containing n-butanol is further separated and eluted by polyamide phenol resin column, the eluate is concentrated until it has no alcohol smell, the pH is adjusted to 2~3, cooled and stood, and filtered to obtain isorhamnetin-3 -O- β -D-rutinose; the isorhamnetin-3-O- that the inventive method makes β -D-rutinose content > 80%, the method has a high extraction rate, low equipment requirements, easy to expand production, and is suitable for industrial production.

Description

technical field [0001] The invention relates to the field of deep processing of natural resources, in particular to a method for extracting and separating rhamnin-3-O-β-D-rutinose from gorse flower buds. Background technique [0002] Caraganasinica (Caraganasinica) is a deciduous shrub of the genus Caragana of the Fabaceae family. Gorse is distributed in the Yangtze River Basin of China and the hills and mountainous sunny slopes of North China. There are more than 40 species produced in China, and the resources are relatively abundant. In Yunnan, winter ends and spring comes, and gorse blooms all over the mountains and fields. Its appearance is not outstanding, but its fragrance is unique. People stir-fry it with eggs, bacon, ham and fresh meat, or cook it with sliced ​​meat and tofu to make a three-flavored soup, which is sweet and delicious. A folk proverb says: "If you eat gorse, your eyes will not blink." Zhang Xiaoling et al. (2004) and Fan Jian et al. (2006) report...

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Problems solved by technology

However, so far, there are few reports on the extraction method of isorhamnetin-3-O-β-D-rutinose, and so far only Li Kunping et al. (2011) used macroporous adsorption resin to enrich the total For flavonoids, use SephadexLH-20 gel column to separate and prepare isorhamnetin-3-O-β-D-rutinoside reference substance with a purity greater than 98% from total flavonoids. The method is simple and effective, but the key step of the method uses gel Gel materials are purified, but the gel has the problems of high market price and small processing volume, which leads to low yield of isorhamnetin-3-O-β-D-rutinose and is not easy to expand production

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