Extraction method of cottonseed protein

Abstract

The invention provides an extraction method of cottonseed protein. The extraction method comprises the following steps of pretreating cottonseeds to obtain cottonseed kernels, and then defatting the cottonseed kernels so as to obtain defatted cottonseed meal; at least performing two stages of leaching extraction on the defatted cottonseed meal with a methanolic solution so as to obtain leached materials, wherein the time of each stage of the leaching extraction is 10-30min, the concentration of the methanolic solution is 85-95%, and the weight ratio of the defatted cottonseed meal to the methanolic solution is 1 to (0.8-2); and then draining the leached materials, and performing desolventizing and drying so as to obtain the cottonseed protein. According to the extraction method of the cottonseed protein disclosed by the invention, quick, high-efficient and thorough removal of non-protein ingredient substances and hazardous substances in cottonseed meal is realized, and the made cottonseed concentrated protein is high in content and high in nutritive efficiency.

Description

technical field [0001] The invention belongs to the technical field of cottonseed processing, and relates to a protein extraction method, in particular to a cottonseed protein extraction method. Background technique [0002] At present, the production methods of cottonseed processing to produce cottonseed protein include traditional hot pressing production and puffing production. The production process of hot pressing and expanding is the process steps of cottonseed cleaning, crushing, separation, steaming and frying, hot pressing (expansion), drying, oil extraction and desolventization. The crude protein content of the cotton meal prepared by this process is 42-46%. Due to the high temperature process used in the production process, the denaturation of cotton protein is large, the solubility of cotton protein potassium hydroxide is low, and its amino acids are destroyed. At the same time, no dephenolization, aflatoxin removal and other anti-nutritional factors, such as ta...

AI Technical Summary

Problems solved by technology

Compared with hot pressing and swelling technology, this process technology has been greatly improved, but in the process of removing phenols, sugars, and aflatoxins, because the leaching frame equipment and methanol soaking treatment method are used, the cottonseed meal is processed in the extractor. The drag box is in a relatively static state of accumulation, and the contact between cotton meal and methanol is insufficient, resulting in poor methanol fluidity in the environment around the meal embryo sheet, which affects the efficient extraction and removal of gossypol, sugar, and aflatoxin contained in cotton meal

If some cottonseeds have high gossypol content due to different growth regions and varieties, or cottonseeds have mildew and have high aflatoxin content, the dephenolized cottonseed protein product prepared by this soaking extraction method will have free gossypol, aflatoxin Toxins are difficult to control within the range of quality standards

There are also production methods in order to increase the protein content of cottonseed and further remove anti-nutritional factors. On the basis of this method, the concentration of methanol is reduced, and cottonseed is extracted with methanol with a concentration of less than 85%. Although this method is effective for increasing the amount of protein , but it is easy to cause cotton meal to absorb water and swell and gelatinize, and the cotton meal embryo flakes loose to form a large number of cotton meal fine particles floating on the solvent, which makes it difficult to filter and recover methanol in the process production. At the same time, the process pipeline and equipment are blocked during production, which affects the It is difficult to achieve large-scale industrial production for normal process production

Moreover, the use of low-concentration and high-dose methanol increases the amount and difficulty of waste methanol recovery and purification, resulting in high energy consumption and high cost.

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