Method for extracting polyphenolic compounds from seabuckthorn fruit
A technology of polyphenolic compounds and seabuckthorn, which is applied in cosmetics, food science, and drug combination, can solve the problems of long extraction time, high cost, and small extraction volume, and achieve short extraction time, less solvents, and polyphenols. The effect of high compound content
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Embodiment 1
[0013] Take 100g of seabuckthorn dried fruit powder or dried seabuckthorn pomace, after deoiling with n-hexane, add 150g of diatomaceous earth and mix evenly, put it in the extraction pool, use 70% ethanol aqueous solution as the extraction solvent, the extraction pressure is 15Mpa, the temperature is At 60°C, extract once for 15 minutes each time. After the extraction is completed, the extract is obtained by filtration, concentrated and freeze-dried to obtain polyphenol compounds in seabuckthorn fruits, with a total polyphenol content of 28.3%. (The determination of the total phenol content of the sample adopts the diazo reagent colorimetric method)
Embodiment 2
[0015] Take 400g of seabuckthorn dried fruit powder or dried seabuckthorn pomace, after deoiling with n-hexane, add 1kg of diatomaceous earth and mix evenly, put it in the extraction pool, use 55% ethanol aqueous solution as the extraction solvent, the extraction pressure is 12Mpa, the temperature is Extract once at 75°C for 10 minutes each time. After the extraction is completed, filter to obtain the extract, concentrate and freeze-dry to obtain polyphenol compounds in seabuckthorn fruit, with a total polyphenol content of 27.0%. (The determination of the total phenol content of the sample adopts the diazo reagent colorimetric method)
Embodiment 3
[0017] Take 400g of seabuckthorn dried fruit powder or dried seabuckthorn pomace, after deoiling with n-hexane, add 800g of diatomaceous earth and mix evenly, put it in the extraction pool, use 85% ethanol aqueous solution as the extraction solvent, the extraction pressure is 20Mpa, the temperature is Extract once at 55°C for 15 minutes each time. After the extraction, filter to obtain the extract, concentrate and freeze-dry to obtain polyphenol compounds in seabuckthorn fruit, with a total polyphenol content of 23.8%. (The determination of the total phenol content of the sample adopts the diazo reagent colorimetric method)
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