Method for extracting polyphenolic compounds from seabuckthorn fruit

A technology of polyphenolic compounds and seabuckthorn, which is applied in cosmetics, food science, and drug combination, can solve the problems of long extraction time, high cost, and small extraction volume, and achieve short extraction time, less solvents, and polyphenols. The effect of high compound content

Inactive Publication Date: 2013-02-06
苏州宝泽堂医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The methods currently used to extract polyphenols from sea buckthorn fruit include microwave extraction, ultrasonic extraction and other methods (Liu Lingling et al., Applied Chemical Industry, 2010,

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Take 100g of seabuckthorn dried fruit powder or dried seabuckthorn pomace, after deoiling with n-hexane, add 150g of diatomaceous earth and mix evenly, put it in the extraction pool, use 70% ethanol aqueous solution as the extraction solvent, the extraction pressure is 15Mpa, the temperature is At 60°C, extract once for 15 minutes each time. After the extraction is completed, the extract is obtained by filtration, concentrated and freeze-dried to obtain polyphenol compounds in seabuckthorn fruits, with a total polyphenol content of 28.3%. (The determination of the total phenol content of the sample adopts the diazo reagent colorimetric method)

Embodiment 2

[0015] Take 400g of seabuckthorn dried fruit powder or dried seabuckthorn pomace, after deoiling with n-hexane, add 1kg of diatomaceous earth and mix evenly, put it in the extraction pool, use 55% ethanol aqueous solution as the extraction solvent, the extraction pressure is 12Mpa, the temperature is Extract once at 75°C for 10 minutes each time. After the extraction is completed, filter to obtain the extract, concentrate and freeze-dry to obtain polyphenol compounds in seabuckthorn fruit, with a total polyphenol content of 27.0%. (The determination of the total phenol content of the sample adopts the diazo reagent colorimetric method)

Embodiment 3

[0017] Take 400g of seabuckthorn dried fruit powder or dried seabuckthorn pomace, after deoiling with n-hexane, add 800g of diatomaceous earth and mix evenly, put it in the extraction pool, use 85% ethanol aqueous solution as the extraction solvent, the extraction pressure is 20Mpa, the temperature is Extract once at 55°C for 15 minutes each time. After the extraction, filter to obtain the extract, concentrate and freeze-dry to obtain polyphenol compounds in seabuckthorn fruit, with a total polyphenol content of 23.8%. (The determination of the total phenol content of the sample adopts the diazo reagent colorimetric method)

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PUM

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Abstract

The invention provides a method for extracting polyphenolic compounds from seabuckthorn fruit. According to the invention, seabuckthorn dried fruit powder or dry seabuckthorn fruit pomace are well mixed with diatomite, and the mixture is subjected to extraction in an extraction tank; filtering is carried out after extraction is finished, such that an extraction liquid is obtained; the liquid is concentrated and lyophilized, such that polyphenolic compounds in seabuckthorn fruit are obtained. The method provided by the invention has the advantages of short extraction time, low solvent dosage, and high extraction efficiency.

Description

technical field [0001] The invention belongs to the technical field of natural plant extraction, and in particular relates to a method for extracting polyphenolic compounds from seabuckthorn. Background technique [0002] Seabuckthorn, also known as vinegar willow and thorny willow, is a shrub or small tree of the genus Acanthopanax in the family Elapidaceae. Seabuckthorn is a general term for plants and their fruits. Seabuckthorn is a medicinal and edible plant. The roots, stems, leaves, flowers, and fruits of seabuckthorn, especially the fruit of seabuckthorn, are rich in nutrients and biologically active substances, and can be widely used in many fields of the national economy such as food, medicine, light industry, aerospace, agriculture, animal husbandry and fishery. Seabuckthorn fruit used as medicine has the effects of relieving cough and reducing phlegm, invigorating stomach and eliminating food, promoting blood circulation and dispelling blood stasis. According t...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61K8/97A61P39/06A61Q19/08A23L1/29A61K131/00A23L33/00
Inventor 李法庆刘东锋
Owner 苏州宝泽堂医药科技有限公司
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