36 results about "Agglutinin-B" patented technology

The present invention provides a genetic method for tethering polypeptides to the yeast cell wall in a form accessible for binding to macromolecules. Combining this method with fluorescence-activated cell sorting provides a means of selecting proteins with increased or decreased affinity for another molecule, altered specificity, or conditional binding. Also provided is a method for genetic fusion of the N terminus of a polypeptide of interest to the C-terminus of the yeast Aga2p cell wall protein. The outer wall of each yeast cell can display approximately 10 protein agglutinins. The native agglutinins serve as specific adhesion contacts to fuse yeast cells of opposite mating type during mating. In effect, yeast has evolved a platform for protein-protein binding without steric hindrance from cell wall components.

The invention relates to the technical field of an affinity material, silica gel is taken as a substrate, a preparation method comprises synthesis of a spacer arm-modified silica gel containing bifunctional groups, and is used for preparing an agglutinin affinity material containing high bonding amount. A structure of the agglutinin affinity material is characterized in that the silica gel is taken as the substrate, the spacer arm end contains function groups of epoxy, hydroxyl, carboxyl, isocyano, and aldehyde group, and a ligand is agglutinin. The prepared agglutinin affinity material has the advantages of simple process, mild condition, low cost, high bonding amount, stable agglutinin property, and good agglutinin activity, and can be widely used for separating and gathering glycoprotein and glycopeptides.

The invention discloses an agent plate for testing hepatocarcinoma and a preparation method and the application thereof. The agent plate is composed of a rigid plate and an agent strip attached to the rigid plate, and the agent strip is formed by sequentially and closely splicing absorbent filter paper, a pyroxylin membrane, a glass fibre membrane and a sample adsorption glass fibre membrane from top to bottom, wherein a gold-labeled anti-mouse AFP monoclonal antibody is absorbed by the glass fibre membrane; an agglutinin coated detection line is arranged at one end of the pyroxylin membrane, which is adjacent to the glass fibre membrane, and a goat anti mouse IgG coated contrast line is arranged at the other end which is adjacent to the absorbent filter paper; and the lower end of the sample adsorption glass fibre membrane is provided with a row of loading holes. The agent plate for testing hepatocarcinoma has the advantages that a fluorescence microscope, an Elisa tester and other expensive instruments are not needed, and the agent plate is much more suitable for field test; the agent plate is safer since radioactive isotopes, TMB and other harmful substances or chemical substances are not needed in testing processes; test results can be preserved for a long time; and the operation of the agent plate is easy and fast, and the agent plate can be operated by a single person.

The invention discloses a method for microwave-assisted reverse micelle separation and purification of kidney bean agglutinin, and relates to a separation and purification method of the agglutinin, aiming at solving the problems of low ratio, high cost, more processes and long time for separation and purification of the kidney bean agglutinin. According to the method for the microwave-assisted reverse micelle separation and purification of the kidney bean agglutinin, the agglutinin is directly separated and purified from kidney bean agglutinin water extract; and the method comprises a pre-extraction process and a back extraction process. Under an auxiliary effect of microwave, water-soluble protein in kidney bean is quickly dissolved into the water extract, so that the dissolubility is greatly improved, and the leaching time is shortened. Based on a liquid-liquid extraction principle, the selectivity of extraction is improved, and high-efficiency pre-extraction and back extraction of the kidney bean agglutinin can be realized. By applying a reverse micelle extraction technology, a better separation and purification effect can be achieved with only the pre-extraction and back extraction steps; the method has simple steps and is convenient to operate; and the pre-treatment time can be greatly shortened in combination with microwave assistance so as to save the cost.

The invention belongs to the technical field of nuclear magnetic resonance, and particularly discloses a magnetic relaxation switch for detecting glycoprotein. A probe of the magnetic relaxation switch adopts a super-paramagnetic nano particle which uses a ferroferric oxide particle as a core, uses glucan as a shell and has average particle size of no more than 60 nanometers. A method for detecting the glycoprotein by using the magnetic relaxation switch comprises two mixing steps and one detecting step, wherein the mixing steps comprises mixing of agglutinin and mixing of target substance, the agglutinin is concanavalin, and the target substance is a1-acid glycoprotein. The linear concentration range of the detection is 0 to 7.0nmol/L, and the detection limit is 0.40nmol/L and is far lower than the normal concentration of AGP in blood plasma.

The invention discloses a novel composite aquatic product fresh-keeping agent, and a preparation method thereof. The novel composite aquatic product fresh-keeping agent comprises 5 to 10 parts of garlic juice, 5 to 10 parts of ginger juice, 30 to 40 parts of tea polyphenol, 10 to 20 parts of crenomytilus grayanus agglutinin, 10 to 20 parts of chitin, 30 to 40 parts of lysozyme, 50 to 60 parts of chitosan, 10 to 15 parts of sodium alginate, and 20 to 30 parts of glacial acetic acid. The preparation method of the novel composite aquatic product fresh-keeping agent is simple; the novel composite aquatic product fresh-keeping agent is prepared via combination of a plurality of natural biological fresh-keeping agents, is capable of inhibiting a plurality of bacteria and microorganisms, possesses excellent sterilizing effect and antibacterial effect, and is wide in effective range, long in lasting time, and high in practicality.

The invention provides a method of producing easily filtered agglutinin; wherein, for the procedure of producing temporary aqueous paste adopting purging operation as the represent when the organic nanoparticle aqueous dispersant is phase changed to form organic nanoparticle non-aqueous dispersant (that is: the procedure of adopting filter to need waste a lot of time), the invention converts the nanoparticle in the organic nanoparticle aqueous dispersant into easily filtered agglutinin. The invention provides a method of producing organic nanometer dye particle agglutinin; wherein the organic nanometer dye particle dispersed in the aqueous medium acts with at least an organic low molecular compound with molecular weight less than 1000 and at least an organic high molecular compound with weight average molar mass more than 1000 to agglutinate the organic nanometer dye particle and then separate.

The invention discloses nutritional pea vermicelli and a preparation method thereof. The nutritional pea vermicelli comprises the following components in parts by weight: 100 parts of pea starch and 0.5-1 part of composite thickening agent, wherein the composite thickening agent consists of the following components in parts by weight: 10-30 parts of Tara gum, 10-30 parts of pullulan and 60-100 parts of hydroxypropyl distarch phosphate. The nutritional pea vermicelli do not contain alum, is high in safety, smooth in surface, difficult to break and paste and rich in abscisic acid, gibberellin, phytohem agglutinin and other substances, has the functions of resisting bacterium, diminishing inflammation and enhancing metabolism, and is stable in product quality, difficult to mildew and long in quality guarantee period.

The invention discloses an application of mussel agglutinin to the preparation of cosmetics. The mussel agglutinin is specific mussel agglutinin (CGL) of N-acetyl galactosamine/galactose (GalNAc/Gal), namely the mussel agglutinin is used as a biological active constituent and is added into skin-care cosmetics. The mussel agglutinin has obvious functions of promoting growth of a fibroblast and protecting the fibroblast from ultraviolet radiation damage, so that the ultraviolet radiation damage can be effectively prevented, and skin ageing and the like caused by the ultraviolet radiation damage can be avoided; the mussel agglutinin is from mussel, so that the raw material of the mussel agglutinin can be obtained easily and is safe and reliable; the preparation process is simple and mature; and the mussel agglutinin is low in cost and convenient to apply.

The present invention relates to a method for testing the interaction of a temperature-sensitive random polymer with agglutinin. The method comprises the steps of: preparing a temperature-sensitive random polymer P (DEGMA-co-OVNGlu) solution; preparing an agglutinin protein Con A solution, an agglutinin protein BSA solution and an agglutinin protein RCA120 solution; UV testing the Con A solution,the BSA solution and the RCA120 solution; UV testing the mutual combination of the polymer with Con A, BSA, RCA120; subjecting different concentrations of polymer solutions to a dynamic light scattering test; and adding the agglutinin solution to the polymer solution and then performing a dynamic light scattering test. Compared with other test methods (surface plasmon resonance and isothermal titration calorimetry), the method is easy to operate, low in cost, and fast in test analysis.

The invention provides a feeder additive capable of increasing animal immunity and meat quality, comprising by weight part: leftovers of edible housefly maggot culture material 100; edible housefly maggot dry-power 1-3; edible housefly imago dry-power 0.3-0.8. The advantages of the invention are that: the housefly maggot fly powder comprises rich chitin, antibiotic peptide, exogenous agglutinin, defensins or the other toxin-expulsion antibiotic substance, multiplex vitamin and trace element which can increase immunity of the animal, in addition, the leftovers of the housefly maggot culture material comprise wheat bran, peanut bran, corn flour, bean pulp, wheat power, milk power and excretion of the housefly maggot, the nutrient is rich, therefore the animal quickly grows up and the meat quality is increased.

The invention provides a micro-array chip based on a sphere-brush double layer nano-structure substrate and a preparation method thereof. The preparation method of the micro-array chip has versatility, and has the advantages of convenient process and less equipment requirement, and is suitable for large batch production; the micro-array chip can realize the analysis detection of interacting between nucleotide and nucleotide, sugar and protein, and protein and protein, and has the advantages of simpleness, less sample consumption and high sensitivity. The experiment result shows that the sugar micro-array chip prepared by the method has the detection limit on biotin-modified castor-oil plant agglutinin-120 and biotin-modified concanavalin agglutinin being 1 ng/mL, and the DNA micro-array chip has the detection limit on target DNA being 0.1 nmol/L; the glycoprotein micro-array chip has the detection limit on the biotin-modified castor-oil plant agglutinin-120 being 0.3 ng/ml, and the antibody micro-array chip has the detection limit on the CY5-modified rabbit-anti-human antibody being 10 pg/mL.

The invention discloses a preparation method of seaweed flour paste. The seaweed flour paste is prepared through ultrafine seaweed powder, quality wheat flour, seaweed leaching liquid and table salt, remains both the sweet of traditional flour paste and flavor of fresh seaweed, and meets the demand of people on tasting the flour paste as well as makes up for the deficiency of eating of seaweed. The ultrafine seaweed powder and the leaching liquid contain polysaccharide, agglutinin, unsaturated fattyacid, iodine and other natural active materials, so that the seaweed flour paste has the effects of resisting virus, tumor, coagulation, thrombus and oxidation and improving immunity of the organism, and shows a high nutritive value after long-term use.

The invention discloses a protein separating and enriching method. The method is characterized by comprising the following steps: subjecting a sample to a primary lectin affinity chromatography, then carrying out an enzymatic hydrolysis on the sample, then subjecting the sample to a secondary lectin affinity chromatography, finally separating the sample by utilizing HPLC, and sequencing with a mass spectrum instrument. The agglutinin used in the method comprises concanavalin A, wheat germ agglutinin, PHA phaseolus vulgaris agglutinin, etc. The glycopeptides in the processed sample is analyzed and retrieved in a database by a capillary liquid chromatography-ESI-MASS or a capillary liquid chromatography-MADLI-TOF-MASS. The method has the advantages that various saccharide species can be collected in one time, the labeled polypeptide can be enriched through an affinity method, and a quantitative and comparative analysis is advantageously to be achieved through adopting an isotope labeled reagent.

The invention discloses a semen film surface grist agglutinin accepter quantization testing agent and agent box, it also provides the corresponding testing method to test the semen film surface grist agglutinin accepter, and it provides the semen film WAG accepter location and amount information which can be directly applied in aphoria.