36 results about "Agglutinin-B" patented technology

The present invention provides a genetic method for tethering polypeptides to the yeast cell wall in a form accessible for binding to macromolecules. Combining this method with fluorescence-activated cell sorting provides a means of selecting proteins with increased or decreased affinity for another molecule, altered specificity, or conditional binding. Also provided is a method for genetic fusion of the N terminus of a polypeptide of interest to the C-terminus of the yeast Aga2p cell wall protein. The outer wall of each yeast cell can display approximately 10 protein agglutinins. The native agglutinins serve as specific adhesion contacts to fuse yeast cells of opposite mating type during mating. In effect, yeast has evolved a platform for protein-protein binding without steric hindrance from cell wall components.

The invention relates to the technical field of an affinity material, silica gel is taken as a substrate, a preparation method comprises synthesis of a spacer arm-modified silica gel containing bifunctional groups, and is used for preparing an agglutinin affinity material containing high bonding amount. A structure of the agglutinin affinity material is characterized in that the silica gel is taken as the substrate, the spacer arm end contains function groups of epoxy, hydroxyl, carboxyl, isocyano, and aldehyde group, and a ligand is agglutinin. The prepared agglutinin affinity material has the advantages of simple process, mild condition, low cost, high bonding amount, stable agglutinin property, and good agglutinin activity, and can be widely used for separating and gathering glycoprotein and glycopeptides.

The present invention provides a genetic method for tethering polypeptides to the yeast cell wall in a form accessible for binding to macromolecules. Combining this method with fluorescence-activated cell sorting provides a means of selecting proteins with increased or decreased affinity for another molecule, altered specificity, or conditional binding. Also provided is a method for genetic fusion of the N terminus of a polypeptide of interest to the C-terminus of the yeast Aga2p cell wall protein. The outer wall of each yeast cell can display approximately 104 protein agglutinins. The native agglutinins serve as specific adhesion contacts to fuse yeast cells of opposite mating type during mating. In effect, yeast has evolved a platform for protein-protein binding without steric hindrance from cell wall components. As one embodiment, attaching an scFv antibody fragment to the Aga2p agglutinin effectively mimics the cell surface display of antibodies by B cells in the immune system for affinity maturation in vivo. As another embodiment, T cell receptor mutants can be isolated by this method that are efficiently displayed on the yeast cell surface, providing a means of altering T cell receptor binding affinity and specificity by library screening.

The invention discloses an agent plate for testing hepatocarcinoma and a preparation method and the application thereof. The agent plate is composed of a rigid plate and an agent strip attached to the rigid plate, and the agent strip is formed by sequentially and closely splicing absorbent filter paper, a pyroxylin membrane, a glass fibre membrane and a sample adsorption glass fibre membrane from top to bottom, wherein a gold-labeled anti-mouse AFP monoclonal antibody is absorbed by the glass fibre membrane; an agglutinin coated detection line is arranged at one end of the pyroxylin membrane, which is adjacent to the glass fibre membrane, and a goat anti mouse IgG coated contrast line is arranged at the other end which is adjacent to the absorbent filter paper; and the lower end of the sample adsorption glass fibre membrane is provided with a row of loading holes. The agent plate for testing hepatocarcinoma has the advantages that a fluorescence microscope, an Elisa tester and other expensive instruments are not needed, and the agent plate is much more suitable for field test; the agent plate is safer since radioactive isotopes, TMB and other harmful substances or chemical substances are not needed in testing processes; test results can be preserved for a long time; and the operation of the agent plate is easy and fast, and the agent plate can be operated by a single person.

The invention relates to an efficient expression method of a human lysozyme. By comprehensively considering various factors affecting expression and then optimizing the cDNA sequence of the human lysozyme, a lysozyme expression box is established by use of the signal peptide of phaseolus vulgaris agglutinin and the mature peptide of the human lysozyme and used for expressing the human lysozyme; and as a result, the expression efficiency of the lysozyme is greatly improved. The lysozyme expressed by the efficient expression method of the human lysozyme is close to the natural lysozyme and is extremely high in enzymatic activity.

The invention discloses a method for microwave-assisted reverse micelle separation and purification of kidney bean agglutinin, and relates to a separation and purification method of the agglutinin, aiming at solving the problems of low ratio, high cost, more processes and long time for separation and purification of the kidney bean agglutinin. According to the method for the microwave-assisted reverse micelle separation and purification of the kidney bean agglutinin, the agglutinin is directly separated and purified from kidney bean agglutinin water extract; and the method comprises a pre-extraction process and a back extraction process. Under an auxiliary effect of microwave, water-soluble protein in kidney bean is quickly dissolved into the water extract, so that the dissolubility is greatly improved, and the leaching time is shortened. Based on a liquid-liquid extraction principle, the selectivity of extraction is improved, and high-efficiency pre-extraction and back extraction of the kidney bean agglutinin can be realized. By applying a reverse micelle extraction technology, a better separation and purification effect can be achieved with only the pre-extraction and back extraction steps; the method has simple steps and is convenient to operate; and the pre-treatment time can be greatly shortened in combination with microwave assistance so as to save the cost.

The invention belongs to the technical field of nuclear magnetic resonance, and particularly discloses a magnetic relaxation switch for detecting glycoprotein. A probe of the magnetic relaxation switch adopts a super-paramagnetic nano particle which uses a ferroferric oxide particle as a core, uses glucan as a shell and has average particle size of no more than 60 nanometers. A method for detecting the glycoprotein by using the magnetic relaxation switch comprises two mixing steps and one detecting step, wherein the mixing steps comprises mixing of agglutinin and mixing of target substance, the agglutinin is concanavalin, and the target substance is a1-acid glycoprotein. The linear concentration range of the detection is 0 to 7.0nmol / L, and the detection limit is 0.40nmol / L and is far lower than the normal concentration of AGP in blood plasma.

The invention discloses a novel composite aquatic product fresh-keeping agent, and a preparation method thereof. The novel composite aquatic product fresh-keeping agent comprises 5 to 10 parts of garlic juice, 5 to 10 parts of ginger juice, 30 to 40 parts of tea polyphenol, 10 to 20 parts of crenomytilus grayanus agglutinin, 10 to 20 parts of chitin, 30 to 40 parts of lysozyme, 50 to 60 parts of chitosan, 10 to 15 parts of sodium alginate, and 20 to 30 parts of glacial acetic acid. The preparation method of the novel composite aquatic product fresh-keeping agent is simple; the novel composite aquatic product fresh-keeping agent is prepared via combination of a plurality of natural biological fresh-keeping agents, is capable of inhibiting a plurality of bacteria and microorganisms, possesses excellent sterilizing effect and antibacterial effect, and is wide in effective range, long in lasting time, and high in practicality.

The invention provides a method of producing easily filtered agglutinin; wherein, for the procedure of producing temporary aqueous paste adopting purging operation as the represent when the organic nanoparticle aqueous dispersant is phase changed to form organic nanoparticle non-aqueous dispersant (that is: the procedure of adopting filter to need waste a lot of time), the invention converts the nanoparticle in the organic nanoparticle aqueous dispersant into easily filtered agglutinin. The invention provides a method of producing organic nanometer dye particle agglutinin; wherein the organic nanometer dye particle dispersed in the aqueous medium acts with at least an organic low molecular compound with molecular weight less than 1000 and at least an organic high molecular compound with weight average molar mass more than 1000 to agglutinate the organic nanometer dye particle and then separate.

The invention discloses an application of mussel agglutinin to the preparation of cosmetics. The mussel agglutinin is specific mussel agglutinin (CGL) of N-acetyl galactosamine / galactose (GalNAc / Gal), namely the mussel agglutinin is used as a biological active constituent and is added into skin-care cosmetics. The mussel agglutinin has obvious functions of promoting growth of a fibroblast and protecting the fibroblast from ultraviolet radiation damage, so that the ultraviolet radiation damage can be effectively prevented, and skin ageing and the like caused by the ultraviolet radiation damage can be avoided; the mussel agglutinin is from mussel, so that the raw material of the mussel agglutinin can be obtained easily and is safe and reliable; the preparation process is simple and mature; and the mussel agglutinin is low in cost and convenient to apply.

The invention discloses a preparation method for an allochroic sensor used for rapid identification and early diagnosis of IgA nephrosis. The preparation method includes obtaining stable butadiyne-type vesicle dispersoid by using a film method or an injection method; dissolving a probe agglutinin in a buffer having pH of 6-8, adding the agglutinin into the butadiyne-type vesicle dispersoid in mol ratio of 1:1 to 1:5; modifying the surface of butadiyne-type vesicle by the agglutinin in the solution in a physical absorption or chemical coupling manner at the temperature of 4-37 DEG C to obtain a tool having a specific recognition function for N-acetylgalactosamine in serum wherein N-acetylgalactosamine is the core fragment of the IgA1 marker having abnormal O-glycosylation. The preparation method is simple, rapid, accuracy and economy, and the price of the product is far lower than that of the like product. The preparation method for an allochroic sensor is used for popularizing the rapid identification, early diagnosis and general examination of IgA nephrosis.

The invention discloses an unicellular organism-based high-hydrophobicity micrometer powder material, and a preparation method thereof. According to the preparation method, unicellular organism cells are washed repeatedly, an activation agent is used for activation of the unicellular organism cells so as to obtain surface activated unicellular organism cells; at the same time, Fe3O4 nanoparticles are subjected to washing and modification so as to realize combination of the nanoparticles with agglutinin molecules, and obtain agglutinin molecule-modified nanoparticles; the agglutinin molecule-modified nanoparticles are combined with the surface-activated unicellular organism cells; construction of unicellular organism surface rough micro-nano structures is realized via immobilizing of the nanoparticles on the surface of the unicellular organism cells; and the surface of an obtained product is subjected to octadecylamine modification so as to reduce particle surface energy further, and obtain the unicellular organism-based high-hydrophobicity micrometer powder material.

The present invention relates to a method for testing the interaction of a temperature-sensitive random polymer with agglutinin. The method comprises the steps of: preparing a temperature-sensitive random polymer P (DEGMA-co-OVNGlu) solution; preparing an agglutinin protein Con A solution, an agglutinin protein BSA solution and an agglutinin protein RCA120 solution; UV testing the Con A solution,the BSA solution and the RCA120 solution; UV testing the mutual combination of the polymer with Con A, BSA, RCA120; subjecting different concentrations of polymer solutions to a dynamic light scattering test; and adding the agglutinin solution to the polymer solution and then performing a dynamic light scattering test. Compared with other test methods (surface plasmon resonance and isothermal titration calorimetry), the method is easy to operate, low in cost, and fast in test analysis.

The invention provides a green and circular cultivation method of chicken eating insects. The method comprises: (1) cultivating chicken which are 1-50 days, keeping the fresh air in the cultivation greenhouse, feeding the chicken which are 1-2 weeks one time each 6 hours and 3-4 times every day, feeding the chicken which are 3-5 weeks one time each 4 hours and 4-5 times every day, feeding the chicken which are older than 5 weeks one time each 2 hours and 5-6 times every day, and performing normal feeding later; and (2) cultivating chicken in growing period which is from 50 days to slaughter, putting the chicken into the forest to perform nature ecological cultivation during the cultivation process. The chicken eating insects uses fly-maggot as forage, and the fly-maggot protein contains a lot of matters which have special effect for human body such as chitin, antibacterial peptide defensin, and exogenous agglutinin, and is rich in a plurality of nutrition components such as nine essential amino acid and protein for human body, free amino acid, vitamin, mineral element, and unsaturated fatty acid, so that the fly-maggot protein is absorbed and converted easily.

The invention provides a micro-array chip based on a sphere-brush double layer nano-structure substrate and a preparation method thereof. The preparation method of the micro-array chip has versatility, and has the advantages of convenient process and less equipment requirement, and is suitable for large batch production; the micro-array chip can realize the analysis detection of interacting between nucleotide and nucleotide, sugar and protein, and protein and protein, and has the advantages of simpleness, less sample consumption and high sensitivity. The experiment result shows that the sugar micro-array chip prepared by the method has the detection limit on biotin-modified castor-oil plant agglutinin-120 and biotin-modified concanavalin agglutinin being 1 ng / mL, and the DNA micro-array chip has the detection limit on target DNA being 0.1 nmol / L; the glycoprotein micro-array chip has the detection limit on the biotin-modified castor-oil plant agglutinin-120 being 0.3 ng / ml, and the antibody micro-array chip has the detection limit on the CY5-modified rabbit-anti-human antibody being 10 pg / mL.

The invention relates to a method to induce nerve stem cell expanding and restrict differentiation toward gial cell. It adopts a serum-free culture system containing a new type grain legumes agglutinin to induce nerve stem cell expanding and restrict differentiation toward gial cell. It provides evidence to nerve cell renewable theory and supplies daughter cell to nerve cell transplant and micro-encapsulation preparation. It could be also used as extraneous pharmacokinetics model to selecting cell medicament. The invention would have great application prospect, and great social and economic benefits.

The invention discloses a preparation method of seaweed flour paste. The seaweed flour paste is prepared through ultrafine seaweed powder, quality wheat flour, seaweed leaching liquid and table salt, remains both the sweet of traditional flour paste and flavor of fresh seaweed, and meets the demand of people on tasting the flour paste as well as makes up for the deficiency of eating of seaweed. The ultrafine seaweed powder and the leaching liquid contain polysaccharide, agglutinin, unsaturated fattyacid, iodine and other natural active materials, so that the seaweed flour paste has the effects of resisting virus, tumor, coagulation, thrombus and oxidation and improving immunity of the organism, and shows a high nutritive value after long-term use.

The invention relates to a method for simultaneously analyzing influenza A virus subtype and virulence thereof by utilizing an agglutinin chip, and the method comprises the following steps: firstly, preparing the agglutinin chip and preparing a biological sample; and then, loading the biological sample to the agglutinin chip to detect the glycoprotein chain in the influenza A virus, thereby rapidly judging the subtype of the influenza A virus and the pathogenicity density of the influenza A virus. The method provided by the invention can be used for solving the technical problem that the conventional method for detecting influenza virus cannot be used for rapidly and simultaneously detecting the influenza A virus subtype and the virulence thereof; and the method provided by the invention has the advantages of rapidness, simplicity, convenience, high efficiency and the like.

The invention discloses a docetaxel polydroxybutyrate nanoparticle, a manufacturing method and apisum sativum agglutinin modification method and application. The manufacturing method of the docetaxel polydroxybutyrate nanoparticle includes the steps that docetaxel and thichloromethane and / or dichloromethane of polyhydroxybutyrate are uniformly mixed and then mixed with an aqueous solution of polyvinyl alcohol, then ultrasonic emulsification is carried out for 3-10 min, curing is carried out, and organic solvents are removed; the mass ratio of the docetaxel to the polyhydroxybutyrate to the polyvinyl alcohol is (0.8-2): (4-10): (20-50). The solubility of the docetaxel is improved, distribution of particle sizes of manufactured products is even, the quantity of carried drugs is large, the packing rate is high, drug release is quick, drugs are released in a controlled mode out of the body, and the bioavailability is high. The docetaxel polydroxybutyrate nanoparticle undergoing apisum sativum agglutinin modification improves drug targeting performance and treatment indexes, and lays a foundation for development of a tumor targeting drug delivery system mediated by specific sugar.

Disclosed is a method for analyzing glycoprotein, which comprises steps of firstly preparing a spotting buffer solution, spotting a chip, closing, cleaning and then drying, finally obtaining an agglutinin chip. A biological specimen is directly added on the agglutinin chip for undergoing steps of incubation, cleaning for removing hybridprotein, forming a crystal and dissociating the crystal, a time-of-flight mass spectrometer is used for detecting the flying time of charged ions in a vacuum electric field so as to obtain the glycoprotein graphical spectrum information, visually display the glycoprotein information such as molecular weight and abundance in the detected biological specimen, and identify the glycoprotein differential expression. Comparing the glycoprotein graphical spectrum information of the detected biological specimen with the sample graphical spectrum information can obtain the glycoprotein information of the differential expression. The method solves the technical problems of low detection flux, complicated detection steps and long cycle in the background technique. The method has advantages of convenient operation, high sensitivity and good repeatability, and is applied to the fast analysis and application in clinic medicine.

The invention discloses matcha powder containing nettle and a preparation method thereof, the matcha powder is prepared from the following raw materials in percentage by weight: 95% of nettle powder and 5% of dandelion powder by sealing the raw materials, filling nitrogen for protection and stirring, the nettle powder is ultrafinely-broken nettle powder obtained by low temperature molecular cell wall breaking, and the dandelion powder is ultrafinely-broken dandelion powder obtained by low temperature molecular cell wall breakin. The matcha powder containing the nettles and the preparation method thereof have the following beneficial effects: 1) the matcha powder has antiviral and immunomodulatory activity, nettle agglutinin has superantigen activity, is capable of inhibiting various viruses and promoting T cell division, not only can improve human immunity, but also can regulate the body's immune system to balance; 2) the nettle is a weak alkaline plant, and can clean up human gastrointestinal trash; 3) the matcha powder can effectively prevent high blood pressure, heart disease and diabetes; 4) the the matcha powder has calcium supplementation effect; 5) the matcha powder has effective prevention and treatment effect on skin diseases; and 6) the matcha powder has effective prevention and treatment effect on rheumatoid arthritis.

The invention relates to the field of the animal biomedical engineering and specifically discloses a preparation method of recombinant pichia pastoris for surface display of porcine epidemic diarrhea virus core antigen COE protein. A yeast alpha-agglutinin surface display system is adopted to express the antigen core gene, namely COE gene, of the PEDV, and the COE protein is anchored on the cell walls of the yeast, and therefore, the preparation method of recombinant pichia pastoris for surface display of porcine epidemic diarrhea virus core antigen COE protein is provided. The recombinant pichia pastoris can be prepared into novel vaccine products having excellent industrialization prospect; the popularization and application of the vaccines play a positive role in preventing viral diarrhea having serious harm to the pig industry and have great practical significance for protecting and promoting healthy development of the pig industry, and meanwhile, remarkable social and economic benefits can be achieved, and therefore, the vaccines have vast market and industrialization prospect.

The invention discloses a protein separating and enriching method. The method is characterized by comprising the following steps: subjecting a sample to a primary lectin affinity chromatography, then carrying out an enzymatic hydrolysis on the sample, then subjecting the sample to a secondary lectin affinity chromatography, finally separating the sample by utilizing HPLC, and sequencing with a mass spectrum instrument. The agglutinin used in the method comprises concanavalin A, wheat germ agglutinin, PHA phaseolus vulgaris agglutinin, etc. The glycopeptides in the processed sample is analyzed and retrieved in a database by a capillary liquid chromatography-ESI-MASS or a capillary liquid chromatography-MADLI-TOF-MASS. The method has the advantages that various saccharide species can be collected in one time, the labeled polypeptide can be enriched through an affinity method, and a quantitative and comparative analysis is advantageously to be achieved through adopting an isotope labeled reagent.

The invention belongs to the molecular biology field, and especially the glycobiology field, which provides a kit for detecting a Tn antigen on a CA125 surface. The invention relates to the kit for detecting an antibody-agglutinin ELISA of Tn antigen on the CA125 surface. The kit can capture the CA125 in serum through the CA125 antibody coated on a 96 orifice plate, the specificity is used for recognizing the agglutinin VVA of Tn antigen to detect the Tn antigen content on the CA125 surface, thereby whether the patient has malignant gynecologic tumor due to rising of CA125 or not can be determined. The time for kit detection is saved, a required serum sample is easy to be acquired, and the kit has high degree of accuracy used for distinguishing rising of benign CA125 and malignant CA125.

The invention relates to a preparation method of an agglutinin-magnetosome composite. The method comprises the following steps: suspending a strain in 1 PBS (phosphate buffer solution) in an M:V ratio of 1:10, crushing with a high-pressure homogenizer (the pressure is 120-140 MPa) 3-4 times, putting into a magnetic field, and carrying out magnetic separation to obtain the magnetosome. Compared with the unmodified magnetosome, the modified PE (polyethylene)-magnetosome has better dispersity and can be coupled with more agglutinin. The prepared WGAPE-magnetosome can well separate the target glycoprotein, and avoids the influence of the magnetosome protein on the glycoprotein separation specificity.

The invention discloses a semen film surface grist agglutinin accepter quantization testing agent and agent box, it also provides the corresponding testing method to test the semen film surface grist agglutinin accepter, and it provides the semen film WAG accepter location and amount information which can be directly applied in aphoria.

Disclosed are particles comprising a complex of nicotine and cerium oxide and a biodegradable coating comprising agglutinin. Also disclosed is a method of treating or preventing neurodegenerative or neurological disorders in a mammal in need thereof, comprising administering to the mammal a therapeutically effective amount of the particles.

The invention discloses an application of mussel agglutinin to the preparation of cosmetics. The mussel agglutinin is specific mussel agglutinin (CGL) of N-acetyl galactosamine / galactose (GalNAc / Gal), namely the mussel agglutinin is used as a biological active constituent and is added into skin-care cosmetics. The mussel agglutinin has obvious functions of promoting growth of a fibroblast and protecting the fibroblast from ultraviolet radiation damage, so that the ultraviolet radiation damage can be effectively prevented, and skin ageing and the like caused by the ultraviolet radiation damage can be avoided; the mussel agglutinin is from mussel, so that the raw material of the mussel agglutinin can be obtained easily and is safe and reliable; the preparation process is simple and mature; and the mussel agglutinin is low in cost and convenient to apply.