68 results about "Gynecologic Tumor" patented technology

The invention discloses an integral detecting reacting board and protein chip agent box of Carcinoembryonic antigen,CEA, 15-3 CA15-3(Carbohydrate Antigen 15-3,CA15-3), CA 125 (Cancer antigen 125,CA125), Squamous cell carcinoma antigen,SCC, Human papillomavirus antibody,HPVAb, beta-human chorionic gonadotropin, beta-HCG and alpha-fetoprotein,AFP, wherein the reacting hole board contains base and reacting hole on the base; the reacting hole contains 2-384 sample holes, 2-200 standard sample holes; the fixing phase carrier is set on the bottom of each reacting hole, which is covered by seven antigen or antibody molecule arrays with anti-CEA, anti-CA15-3,anti-CA125,anti-SCC,HPV,anti- beta-HCG,anti-AFP.

The invention provides gynecological tumor extraction forceps with a cutting apparatus. The gynecological tumor extraction forceps comprise a first forceps arm and a second forceps arm hinged together, a finger ring is arranged at the rear end of each of the first and second forceps arms, a threaded sleeve is arranged on the inner side of the rear end of each of the first forceps arm and the second forces arm, an adjusting rod is connected in each threaded sleeve through a thread, a cutter bar is connected to the top of the inner end of each adjusting rod, the cutter bars are mounted in inner cavities of the first forceps arm and the second forceps arm, a connecting sleeve is arranged at the top end of each cutter bar, the inner end of each adjusting rod is arranged in the corresponding connecting sleeve, the other end of each cutter bar is provided with a blade, the tail end of the adjusting rod is provided with an adjusting wheel, and limiting columns and sheaths used for limiting the cutter bars are further arranged inside the first forceps arm and the second forceps arm. The gynecological tumor extraction forceps have the advantages that the forceps are simple in structure and convenient to use, when tumors are cut, the cutter bars eject out the blades by rotating the adjusting wheels for tumor cutting, the blades are brought back by reversely rotating the adjusting wheels, and the function of the extraction forceps is given into play to take out the cut tumors.

The invention provides application of gamabufotalin and a salt compound thereof in preparation of a medicine for treating gynecological tumor. By comparing in vitro anti-cancer experiments of a bufadienolide extract and monomeric compounds, namely the gamabufotalin, cinobufagin, bufalin and bufotalin as well as hydrochlorides or sulfates of the four compounds to four gynecological tumors, namely human ovarian cancer cells A2780, human ovarian cancer cells SK-OV-3, human cervical carcinoma cells and human endometrial cancer cells, an experimental result shows that the gamabufotalin has stronger activity against gynecological tumor cells than the bufadienolide extract and the monomeric compounds, namely the cinobufagin, the bufalin and the bufotalin, and the hydrochloride or the sulfate of the gamabufotalin has stronger activity against gynecological tumor cells than the hydrochlorides or the sulfates of three compounds, namely the cinobufagin, the bufalin and the bufotalin. The gamabufotalin and the salt compound thereof provided by the invention have the advantages of clear components, controllable quality, strong anti-cancer activity, and small adverse reaction, and are expect to be developed into a novel anti-cancer drug.

The invention discloses a gynecological tumor intra-cavity radiation therapy protecting device, for providing the protecting devices K, G, Q formed by vaginal speculum (1), uterine cavity source tube (2), and vaginal ball source device (3) to form the gynecological tumor intra-cavity radiation therapy protecting system. The inventive technique method comprise: a main protective jacket 9(10), and a main protective side plat 11(12) are arranged on the tubular vaginal speculum (1) to form the sealed protective device (K) of vaginal speculum; a protective tube (13), a pipe casing (14), a lock nut (15) and a bolt (16) are arranged on the uterine cavity source tube (2) to form the protective device G; a semi-circle ball protective jacket 20(21), and a semi-circle protective bottom board 22(23) are arranged on the vaginal ball source device (3) to form the protective device Q. The invention utilizes special protective material and the protective device made via masking technique, therefore, it has the advantages of safety, reliability, and protective function to meet the tracing protection of clinic radiation therapy and reduce the hurt and pain on the normal apparatus of sicker caused by radioactive source side effect. The invention can apply the protective application of hospital gynecological tumor intracavity radiation therapy.

The present invention relates to a traditional Chinese medicine composition for treating gynecologic tumors, especially to a traditional Chinese medicine composition for treating hysteromyoma, and further relates to a preparation method and pharmaceutical uses of the composition. According to the present invention, the traditional Chinese medicine composition is prepared from milkvetch root, common yam rhizome, angelica, rhizoma sparganii, curcuma zedoaria, anemarrhena asphodeloides bunge, semen persicae and chicken's gizzard membrane, can effectively treat gynecological tumors and / or related diseases such as hysteromyoma and the like, can provide the same effective treatment effect based on the less drug, and further has the reasonable drug ratio.

The invention provides application of a bufadienolide compound and a bufadienolide salt compound in preparing a medicine for treating gynecological tumor. Anti-cancer experiments of four gynecological tumor, namely human ovarian cancer cells (A2780), human ovarian cancer cells (SK-OV-3), human cervical carcinoma cells (SiHa) and human endometrial cancer cells (shikawa) are carried out in vitro through a bufadienolide extract and monomeric compounds, namely cinobufagin, bufalin, bufotalin and gamabufotalin as well as hydrochlorides or sulfates of the four monomeric compounds, and an experimental result shows that the bufadienolide extract, the monomeric compounds, namely the cinobufagin, the bufalin, the bufotalin and the gamabufotalin, and the hydrochlorides or the sulfates of the four monomeric compounds have strong inhibition effect on four gynecological tumor cells. The anti-cancer activity of the bufadienolide compound and the bufadienolide salt compound is stronger than that of a positive drug paclitaxel, and the bufadienolide compound and a bufadienolide salt compound have small adverse reaction and expect to be developed into a novel anti-cancer drug.

The invention belongs to the field of traditional Chinese medicine, and more specifically relates to a traditional Chinese medicine composition used for treating gynecological oncology, and a preparation method thereof. The traditional Chinese medicine composition is composed of thallus laminariae, bryozoatum, radix scrophulariae, concha ostreae, spina gleditsiae, bulbus fritillariae thunbergii, rhizoma sparganii, rhizoma curcumae, licorice root, radix bupleuri, fructus aurantii immaturus, cassia twig, cortex moutan, spreading hedyotis herb, poria cocos, radix paeoniae rubra, rhizoma corydalis, pseudobulb of appendiculate cremastra, eupolyphaga sinensts, semen vaccariae, radix curcumae, and ligusticum wallichii. The preparation method comprises following steps: the above medicinal materials are mixed so as to obtain a medicinal material mixture; the medicinal material mixture is grinded into a powder; and the powder is prepared into a composition dosage form. The traditional Chinese medicine composition can be used for treating breast tumor, cystic ovarian tumor, and myoma of uterus, and is capable of avoiding operative treatment of patients.

The invention discloses a culture medium for culturing primary cells of gynecological tumors. The culture medium for culturing the primary cells of the gynecological tumors is a special serum-free culture medium, and the culture medium is used for carrying out in-vitro suspension culture on solid tumor cells from the gynecological tumors, so that the interference of normal cells can be eliminatedto the maximum extent while the tumor cells normally amplify. A culture of the primary cells of the gynecological tumors, which are obtained by utilizing the method disclosed by the invention, can beused for in-vitro experiments at various cell levels, second-generation sequencing, animal model construction, cell line construction and the like. Foreseeably, the culture method has a wide application prospect in the fields of research and clinical diagnosis and treatment of the gynecological tumors.

The invention discloses a culture method of gynecological tumor primary cells. The invention provides a gynecological tumor primary cell culture method and a matched reagent. The core of the technology is as follows: a gynecological tumor solid tumor tissue is treated with a mild cell dissociation reagent, so that the activity of tumor cells in the tissue is ensured to the maximum extent; and a special serum-free culture medium is prepared, and solid tumor cells from a gynecological tumor are cultured in vitro by using a suspension culture system, so that interference of normal cells is eliminated to the maximum extent while normal amplification of the tumor cells is guaranteed. A gynecological tumor primary cell culture obtained by the method can be used for various cell-level in-vitro experiments, second-generation sequencing, animal model construction, cell line construction and the like. It is foreseeable that the culture method has wide application prospects in the fields of gynecological tumor research and clinical diagnosis treatment.

The invention relate to a composite nanometer lipid drug delivery system and therapeutic effect thereof on gynecologic tumor, and particularly provides a composite nanometer lipid drug delivery systemincluding (a) taxol as a first active component, (b) targeted inhibitor miRNA as a second active component and (c) a lipid material, with the components (a), (b) and (c) forming composite lipidosomethe average particle size of which is 10-1000 nm. In addition, the system can also include triptolide and curcumin. The system can reduce toxicity of antitumor drugs, enhance antitumor effect, and reduce drug resistance, thus improving patient's compliance.

The invention discloses a method for preparing a lithospermum extract and application of the lithospermum extract, and relates to the field of medicines. The invention aims at disclosing the method for preparing the lithospermum extract and application of the lithospermum extract to preparing a medicine for treating tumors. The method comprises the following steps: 1, preparing lithospermum powder; 2, extracting effective parts mainly being naphthoquinone compounds of the lithospermum powder obtained in the step 1 by utilizing a CO2 supercritical extraction technology, thus obtaining the wine-colored thick-cream lithospermum extract. The application of the lithospermum extract is the application of the lithospermum extract in preparing the medicine for treating tumors. According to the invention, gynecologic tumors and digestive tract cell lines are emphatically researched and the research result verifies that the effective parts of lithospermum naphthoquinone have exact biological activity for inhibiting growth and inducing apotosis to tumor cell lines, thereby providing a scientific experimental basis for developing hard lithospermum and the effective parts of lithospermum naphthoquinone into a new anticancer drug with a remarkable curative effect and specific targets.

The invention provides traditional Chinese medicine composition for treating gynecological tumor as well as a preparation method and an application of the traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from raw materials in parts by weight as follows: 5-30 parts of rhizoma sparganii, 5-30 parts of rhizoma curcumae, 5-20 parts of ginger leaves, 5-20 parts of radix angelicae sinensis, 5-20 parts of radix astragali, 3-15 parts of rhizoma nardostachyos, 3-15 parts of fructus gardeniae, 3-10 parts of radix clematidis and 3-10 parts of aralia apioides. All the medicines cooperate to have effects of breaking blood, promoting qi circulation, removing qi stagnation, activating blood, removing blood stasis, tonifying blood, clearing heat, removing toxins and clearing and activating channels and collaterals, the traditional Chinese medicine composition is used for treating gynecological tumor such as vulva tumor, vagina tumor, cervix tumor, endometrial cancer, ovarian cancer, cervical cancer, ovarian tumor or fallopian tube tumor, particularly, uterine fibroid, endometrial cancer, ovarian cancer or cervical cancer and has no toxic or side effects, and the prescription is simplified.

The invention belongs to the technical field of medical instruments, and particularly relates to a tumor sampling device for gynecology department. The tumor sampling device for gynecology departmentcomprises a casing, wherein the bottom end of the inner wall of the casing is connected with slide blocks; a cutter is glidingly connected onto the inclined surface of each slide block; a slide rod isfixedly connected onto the inclined surface of each slide block; the top end of the upper surface of each cutter is connected with a positioning plate; each positioning plate is glidingly connected with the corresponding slide rod; the middle part of the top end of each cutter is fixedly connected with a push rod; the top end of each push rod is rolling connection with a rolling ball; a press plate is glidingly connected inside the casing in each rolling ball position; the bottom surface of each press plate is connected with a position limiting rod; each position limiting rod is glidingly connected with the top end of the corresponding push rod; and a piston and a press rod are fixedly connected onto the top surfaces of the press plates. The tumor sampling device for gynecology departmenthas the advantages that a cutting device is arranged in the tumor sampling device, so that the cutters can accurately and fully cut a tumor; meanwhile, cut tumor samples are effectively stored; and the stability and convenience of the tumor sampling device for gynecology department in the work process are greatly improved.

The invention relates to the technical field of gynecological tumor rehabilitation, in particular to a physical exercise device for gynecological tumor rehabilitation, which comprises a base. A seat plate is slidably connected to the base, a first lifting mechanism matched with the seat plate is arranged in the base, a back plate is rotatably connected to the seat plate, a first rotating mechanism matched with the back plate is arranged in the seat plate, a first massage mechanism matched with the seat plate is arranged on the seat plate, the first massage mechanism is connected with the first rotating mechanism, a pair of pedal plates is slidably connected to the base, a second exercise mechanism matched with the pedal plates is arranged in the base and connected with the first massage mechanism, a positioning column is fixedly connected to the base, a first supporting block is fixedly connected to the other end of the positioning column, a movable handle is rotatably connected to the first supporting block, a first exercise mechanism matched with the movable handle is arranged in the first supporting block, and the first exercise mechanism is connected with the second exercise mechanism. The problems of a physical exercise device for gynecological tumor rehabilitation in the prior art are effectively solved.

The invention discloses a fumigating device for relieving gynecological tumor pain. The device not only can facilitate fumigation of a patient and relieve pain of the patient, but also can facilitate daily nursing of the patient. The fumigating device comprises a chair body and a transfer frame, a backrest is rotationally connected to the chair body, a massage device is arranged on the backrest, a dispensing barrel is placed in the chair body, an end cover is detachably connected to the upper end of the dispensing barrel, air outlet holes are formed in the end cover, a driving block is fixedly connected to the backrest, a driving groove is formed in the driving block, a driving pin is slidably connected into the driving groove, the driving pin is fixedly connected with a first sliding block in front-back sliding connection with the chair body, the chair body is provided with a second sliding block capable of being adjusted front and back, the second sliding block is rotationally connected with a worm gear and a worm which are meshed with each other, the eccentric position of the worm gear is rotationally connected with a driving rod, and the end, away from the worm gear, of the driving rod is rotationally connected with the first sliding block. The device is convenient to use, the working intensity of medical staff can be greatly relieved, the working efficiency is improved, the pain of the patient is relieved, and rehabilitation of the patient is accelerated.

The present invention is related to novel use of 2-oxo-heterocyclic compounds having anticancer activity and the process for preparing them and a pharmaceutical composition comprising the same. The present invention provides a pharmaceutical composition for preventing and treating the cancer disease comprising lung cancer, bone cancer, pancreatic cancer, skin cancer, cancer of the head and neck, cutaneous or intraocular melanoma, uterine cancer, ovarian cancer, rectal cancer or cancer of the anal region, stomach cancer, colon cancer, breast cancer, gynecologic tumors, Hodgkin's disease, cancer of the esophagus, cancer of the small intestine, cancer of the endocrine system, sarcomas of soft tissues, cancer of the urethra, cancer of the penis, prostate cancer, chronic or acute leukemia, solid tumors of childhood, lymphocytic lymphonas, cancer of the bladder, cancer of the kidney or ureter, or neoplasms of the central nervous system, therefore, it can be used as the therapeutics for treating and preventing cancer diseases.

The invention discloses a gynecological tumor detector capable of relieving the nervous mood of a patient, and belongs to the technical field of human health examination.The gynecological tumor detector comprises a detection bed board, a working frame is fixedly connected to one end of the detection bed board, two supporting mechanisms are fixedly connected to the upper side of the working frame, and a lighting mechanism is arranged on the outer side of the working frame; a to-be-detected person lies above the detection bed board, two legs of the to-be-detected person are placed above supporting arc rods of the two supporting mechanisms respectively, rising and falling of a supporting circular plate are controlled by controlling extension and contraction of an electric telescopic rod, and then rising and falling of a supporting cylinder, a supporting inclined rod and the supporting arc rods are controlled; the height of the position of the supporting arc rod is proper, the comfort of a person to be examined is improved, a blanket and other shelters are placed above the legs and the abdomen of the person to be examined, light supplementing illumination is conducted through the illumination mechanism, normal examination can be conducted without completely opening one end of each shelter, and the nervous mood of the person to be examined in the examination process is reduced.

The invention discloses a gynecological oncology auxiliary-examination device which comprises a guiding rod. The guiding rod is held to gradually go into the vagina from a conical search-lighting part, a pump body and a flow control valve can be opened in the entering process of the guiding rod to make a disinfectant enter the vagina for disinfection, meanwhile a control switch is turned on to make an illumination lamp emit light and light up the inside of the vagina, then an air bag is inflated through an air nozzle, the air bag is in contact with the inner wall of the vagina after inflation,and the vagina is expanded so that an observation space can be increased, and a medical worker can perform observation and diagnosis through a through hole. The gynecological oncology auxiliary-examination device facilitates simultaneous execution of vagina expansion, disinfection and observation, working steps are decreased, the working difficulty is reduced, and time and effort are saved.

The invention relates to the technical field of medical instruments, and discloses a gynecological tumor nursing infusion pump convenient to carry. Thegynecological tumor nursing infusion pump comprises a fixed shell, wherein two round holes are formed in each of the bottom and the top of the fixed shell, first rotating rods are rotationally connected to the upper surface of the interior of the round hole in the lower left side and the lower surface of the interior of the round hole in the upper right side, and the outer side wall of the first rotating rod is fixedly connected with a plurality of first clockwork springs. According to the invention, by arranging a fixed shell, a first rotating rod, a second rotating rod, a first screw rod, a second screw rod, a first semicircle, a second semicircle, a first pull rope, a second clamping head, a fourth pull rope, a first clamping block, a sliding rod, a pressing plate, a first clockwork spring, a sixth rotating rod, a first round block, a second round block, a long strip, a first fixing plate and a second fixing plate, the long strip is rotated to drive the first round block and a fifth rotating rod to rotate, in this way, the fourth pull rope is tightened, the second clamping head is pulled, and then the clockwork spring drives the first rotating rod and the second rotating rod to rotate, so that the first screw rod is in threaded connection with the first semicircular block.

The invention discloses a protection device for applying intracavity after-loading treatment to gynecological malignant tumors. The protection device comprises a fixing plate, a threaded sleeve, an avoiding hole, a sliding column, a fixing frame A, a water injection blocking bag A, a fixing block, a T-shaped column, a threaded rod, a connecting frame, a fixing frame B, a water injection blocking bag B, a Y-shaped water pipe and a water pump. According to the protection device for applying intracavity after-loading treatment to gynecological malignant tumors, firstly, through the ingenious design of an expansion mechanism, the vagina area of a gynecological patient can be opened, and inspection and operation in the vagina are facilitated; the fixing effect is achieved in cooperation with the effect of the fixing frame; secondly, the bladder and rectum area of the patient can be well blocked and protected and the irradiation dose endangering organs can be reduced in cooperation with the effect of the liquid injection type water bag blocking structure, thereby ensuring after-loading treatment safety; and in addition, the problems that the accuracy of the irradiation dose of a target region is reduced, the repeatability is poor and the like due to the fact that the existing clinical gauze packing is prone to position inconsistency each time are solved, and finally popularization and application of gynecologic tumor treatment are facilitated.

The invention provides a medical recovery bed. The medical recovery bed comprises a bed body and a heat preservation protective cover; the heat preservation protective cover can be arranged on a bed plate of the bed body in an open or close mode, so that when being closed, the heat preservation protective cover encloses at least a part of the bed plate into a corresponding heat preservation space.Therefore, a patient can directly lie on the bed body after a gynecological tumor operation, the body of the patient can be covered by the heat preservation protective cover, and then a heat preservation effect is achieved while the heat preservation protective cover cannot be in contact with the body or a wound of the patient, so that the problems that pain is caused to a patient and operation recovery is not facilitated due to friction of a cover and a wound during an in-bed nursing process of the patient after a gynecological tumor operation in the prior art are solved.

The invention aims to provide a gynecological tumor acupoint treatment device which is safe and reliable, does not has toxic and side effects, can perform acupoint catgut embedding paste treatment to patients under painless condition, can be used by most human bodies for acupoint catgut embedding paste treatment, and is effective to gynecological tumors caused by various reasons. The gynecological tumor acupoint treatment device comprises acupoint catgut and acupoint paste, wherein the acupoint catgut is absorbable protein catgut, and the acupoint paste comprises traditional Chinese medicine components of lumbricus, monkshood, arisaema consanguineum schott, ligusticum wallichii, eucommia ulmoides, frankincense, myrrh, angelica sinensis, radix angelicae tuhuo, rhizoma corydalis, safflower carthamus, dragon's blood, scorpio, centipede, leech, caulis spatholobi, and the like.

The invention discloses protection equipment for intracavitary radiotherapy on gynecolgical tumors. The protection equipment comprises a first shell, wherein a second shell is fixedly arranged at oneend of the first shell, the outer wall of the end, away from a lead screw, of a threaded cylinder is sleeved with a second limiting ring which is fixedly connected with the inner wall of the first shell, the outer wall of the end, close to the lead screw, of the threaded cylinder is sleeved with a third limiting ring which is fixedly connected with the inner wall of the second shell, and limitinggrooves matched with limiting protrusions are formed in the upper ends and the lower ends of the second limiting ring and the third limiting ring. The protection equipment has the advantages that a tumor part can be sucked into the first shell through negative pressure, radiotherapy can be conducted on a tumor part at a fixed point, and therefore anti-radiation protection can be conducted on healthy tissue around the tumor part, the size of the suction part can be adjusted according to the size of the tumor part, and therefore, radiotherapy of tumors of different sizes can be adapted to; and besides, the equipment is easy to operate, and the process of clamping the tumor part is omitted, thereby avoiding the pains brought to patients.

The invention discloses tumor stripping equipment for the gynecological tumor surgery. The equipment comprises an outer cylinder, one end of the outer cylinder is fixedly connected with a fixing rod, the fixing rod is rotatably sleeved with two rotating plates, and one sides of the two rotating plates are fixedly connected with a same C-shaped plate. When the tumor stripping equipment is used, a grip is rotated to drive two rotating shafts to rotate, so that two clamps are matched with a spring rod and a clamping plate to clamp a tumor for stripping, the action amplitude is small, the clamps can be fixed by means of self-locking of the lead screw, fine operation is facilitated, and the life safety of a patient is guaranteed; a rotating handle is rotated to drive adjusting ropes wound on two reels to be wound and unwound one by one, the C-shaped plate is driven to rotate around the fixing rod, the angle of the front end is adjusted, tumors at all positions can be clamped conveniently, the practicability of the equipment is improved, and when a linkage shaft rotates, the linkage shaft can be kept fixed under small external force through an elastic plug pin; the front end of the equipment can be kept relatively stable, and the operation stability is guaranteed.

The invention relates to a medicine for treating gynecologic inflammation. The medicine is prepared from the following components in parts by weight: 25-35 parts of safflower carthamus, 15-20 parts of cordate houttuynia, 15-20 parts of radix sophorae flavescentis, 10-15 parts of borneol, and 20-30 parts of hedyotis diffusa. The medicine is prepared by the following preparation technical flow: selecting the raw material compositions according to the ratio range, mixing the raw materials, adding water in the materials to be decocted for two times, combining the filtrates, concentrating the combined filtrates, adding ethanol in the liquid to be stirred uniformly, standing the liquid for a while, taking a supernatant to be concentrated again, adding starch and the borneol in the concentrate, drying the mixture, pelletizing, and filling the pelletized powder in capsules to finish the production. The medicine disclosed by the invention is suitable for treating chronic gynecologic inflammation and gynecological oncology.

The invention relates to a gynecological capsule which is prepared from the following raw materials in parts by weight: 25-35 parts of solidago canadensis, 15-25 parts of ligusticum wallichii, 15-25 parts of radix sophorae flavescentis and 25-30 parts of leonurus artemisia. A preparation process of the gynecological capsule comprises the following steps: mixing the raw materials in proportion, adding water for decocting twice, combining filtered liquor, concentrating, adding ethanol, uniformly stirring, standing to obtain a supernatant liquor, concentrating again, adding starch, drying, granulating, and capsulating. The gynecological capsule is suitable for chronic gynecological inflammation and gynecological tumors.

The invention belongs to the technical field of medical devices, and specifically relates to a gynecological tumor sampling device, which includes a housing; a slider is connected to the bottom end of the inner wall of the housing, and a cutter is slidably connected to the inclined surface of the slider. A slide bar is fixedly connected to the inclined surface of the slider, a positioning plate is connected to the top of the upper surface of the cutter, and the positioning plate is slidably connected to the slide bar, and a push rod is fixedly connected to the middle part of the top of the cutter, so The tops of the push rods are all rollingly connected with rolling balls, and the inside of the shell at the rolling balls is slidably connected with a pressure plate, and the bottom surface of the pressure plates is connected with a limit rod, and the limit rod is slidably connected with the top of the push rod. The top surface of the pressing plate is fixedly connected with a piston and a pressing rod; the present invention sets a cutting mechanism in the tumor sampling device, so that the cutting knife can accurately and fully cut the tumor, and at the same time, effectively preserve the cut tumor sample, which greatly improves the quality of the tumor. The working stability and convenience of the gynecological tumor sampling device are improved.

The invention discloses a gene library construction method of hereditary gynecologic tumors and a kit and relates to mutation of 20 genes including ATM (ATM serine / threonine kinase), BARD1 (BRCA1 associated RING domain 1), BRCA1 (BRCA1, DNA repair associated), BRCA2 (BRCA2, DNA repair associated), BRIP1 (BRCA1 interacting protein C-terminal helicase 1), CDH1 (cadherin 1), CHEK2 (checkpoint kinase2), NBN (nibrin), PIK3CA (phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha), PALB2 (partner and localizer of BRCA2), PTEN (phosphatase and tensin homolog), RAD51C (RAD51 paralogC), RAD51D (RAD51 paralog D), STK11 (serine / threonine kinase 11), TP53 (tumor protein p53), MSH2 (mutS homolog 2), MLH1 (mutL homolog 1), MSH6 (mutS homolog 6), PMS2 (PMS1 homolog 2, mismatch repair system component) and EPCAM (epithelial cell adhesion molecule). Target regions comprise exon regions of coding amino acids of the above 20 genes and 20 basic group regions at each of the upstream anddownstream of each exon. In order to ensure that all the target regions are covered, a primer group necessary for a PCR (Polymerase Chain Reaction) amplification process is separated into two independent primer groups for carrying out target region amplification respectively. Then a PCR product is subjected to sequencing label connection, library elution and reamplification and purification afteramplification to obtain a library for sequencing. The library construction method disclosed by the invention is simple and rapid in steps; the library construction cost is effectively reduced; and thelibrary covers related genes of the hereditary gynecologic tumors. A high-throughput sequencing instrument is combined and related gene variation can be rapidly and accurately obtained; and the genelibrary construction method has important significance on the hereditary gynecologic tumors.

The invention discloses a medical application of isorupestonic acid for treating gynecological tumors. The medical application discovers that Gui curcumalactone, isorupestonic acid, 7-aromadendrene-6-ketone and 4-aromadendrene have excellent proliferation-inhibiting effect for human ovarian cancer SKOV3 cells and human cervical cancer Hela cells; ledene is a dewatered product of ledol from chemical structure, the chemical structures are more similar, but the difference of the human ovarian cancer SKOV3 cells and the human cervical cancer Hela cells is larger in proliferation-inhibiting activity, wherein the ledene has obvious proliferation-inhibiting effect for the human cervical cancer Hela cells, and has no obvious proliferation-inhibiting effect for the human ovarian cancer SKOV3 cells,but the ledol has obvious proliferation-inhibiting effect for the human ovarian cancer SKOV3 cells, and has no obvious proliferation-inhibiting effect for the human cervical cancer Hela cells. All the ledene, the ledol, the Gui curcumalactone, the isorupestonic acid, the 7-aromadendrene-6-ketone and the 4-aromadendrene can be used for treating the gynecological tumors.

The invention aims at providing external-use composition for mainly treating postpartum diseases and a method for preparing the same. The composition comprises the following components of big dried rehmannia root, white pepper, Chinese date, flavescent sophora root, clove, rhubarb and lotus leaf and mainly treats postpartum diseases, and the composition has curative effects for treating the endocrine dyscrasia, the gynecologic inflammation, the sterility and the gynecologic tumor in women. The weight portions of components of the composition are as follows: 3 to 10 portions of big dried rehmannia root, 2 to 8 portions of white pepper, 3 to 10 portions of Chinese date, 2 to 8 portions of flavescent sophora root, 3 to 10 portions of clove, 3 to 10 portions of rhubarb and 3 to 10 portions oflotus leaf. The method for preparing the composition is as follows: all components of the composition are ground, mixed and packaged, and the finished products are obtained. The composition can be made in all medicament forms such as pills and suppositories. The composition has the advantages of having wide curative effect, mainly treating postpartum diseases and having curative effects for treating the endocrine dyscrasia, the gynecologic inflammation, the sterility and the gynecologic tumor in women; the composition is directly and externally used on human bodies, so that the utilization rate of active components is high and the curative effect is obvious; the composition is prepared by Chinese herbal medicines, so that the composition has no toxic and side effects on human bodies and is safe and reliable.