The invention provides kidney-tonifying and waist-strengthening fitness wine and a preparation method thereof, and relates to the field of traditional Chinese medicines. The kidney-tonifying and waist-strengthening fitness wine is prepared from multiple medicine raw materials, wherein rhizoma curculiginis, herba epimedii, actinolite, herba cistanche, cortex eucommiae, radix achyranthis bidentatae,radix dipsaci, herba cynomorii, testis et penis canitis, rhizoma polygonati, fructus corni, gold silkworm moth and the like have the efficacy of warming kidney and strengthening yang; a synergistic effect is achieved by means of compatibility of multiple medicines; the fitness wine has a better treatment effect for symptoms such as soreness and weakness of waist and knees, impotence, spermatorrhea and premature ejaculation which are caused by deficiency of the kidney; furthermore, the kidney-tonifying and waist-strengthening fitness wine also has the effects of tonifying kidney and strengthening waist on the basis of treatment, and can enhance the functions of the kidney. Meanwhile, the medicine raw materials also include medicines such as fructus lycii, white paeony root and liquorice root which have functions of replenishing blood and tonifying qi, so that the fitness wine has the effects of clearing and activating the channels and collaterals, relieving pain and reducing inflammation and enhancing body immunity. The preparation method of the kidney-tonifying and waist-strengthening fitness wine is simple in preparation way and high in utilization rate of effective components inthe traditional Chinese medicines; the prepared kidney-tonifying and waist-strengthening fitness wine is better in curative effect.