The present invention relates to a large-size and high specific stiffness truss off-axis three-reflection optical system main support structure which mainly comprises a, front plate, a first A-type truss, a second A-type truss, a third A-type truss, a fourth A-type truss, a fifth, A-type truss, a sixth A-type truss, a seventh A-type truss, and a back plate. The technical point is that the seven groups of A-type trusses are combined to the front plate and the back plate through the mode of a threaded connection. Each of the A-type truss comprises an A-type joint, a truss rod, and an I-type joint. The truss rod and the joint are combined together through the mode of glue connection, the mode of glue connection is a sleeve mode, the joint is outside, and the truss rod is inside. The A-type joint is fixed on the front plate, and the I-type joint is fixed at the back plate. The large-size and high specific stiffness truss off-axis three-reflection optical system main support structure has the advantages of compact structure, easy formation of each structure member, easy assembly of a main support structure and a high specific stiffness and can satisfy the use requirement of a large size off-axis three-reflection optical system. The main support structure can be used in the field of large space sensors, space-based telescopes and the like.