Method for producing tea wine by utilizing eurotium cristatus
A technology for R. coronoides and tea wine, which is applied in the field of beverage wine brewing, can solve the problems that tea health care function cannot be fully exerted, and the content of active ingredients of tea polyphenols is low, and achieves the effect of improving the utilization rate of effective ingredients and good quality.
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Embodiment 1
[0014] A method for producing tea wine by utilizing the saccharomyces coronoides, comprising the following steps:
[0015] (1) Preparation of tea solid culture medium: add 5mg of water to 50g of tea leaves, and sterilize under microwave 400w for 60s;
[0016] (2) Fungal fermentation of tea leaves: Physiocystis coronoides FBKL3.0002 ( Eurotium cristatus FBKL3.0002) was activated on PDA solid medium, enriched and cultured in PDA liquid medium at 30°C for 5-7d. Insert 15% into the solid culture medium of tea leaves according to the mass ratio of tea leaves, and ferment at 30°C for 10-15 days;
[0017] (3) Preparation of tea solid culture medium: add 1000mg of boiling water to the fermented tea leaves, then add 160g of brown sugar, soak for 20min, and cool down;
[0018] (4) Yeast fermentation of tea leaves: Schizosaccharomyces kuri ( Schizosaccharomyces spombe CICC1360) was activated on wort solid medium, cultured on wort solid slant medium for 2-3 days, then inserted into w...
Embodiment 2
[0021] A method for producing tea wine by utilizing the saccharomyces coronoides, comprising the following steps:
[0022] (1) Preparation of tea solid culture medium: add 5mg of water to 50g of tea leaves, and sterilize under microwave 400w for 60s;
[0023] (2) Fungal fermentation of tea leaves: Physiocystis coronoides FBKL3.0002 ( Eurotium cristatus FBKL3.0002) was activated on PDA solid medium, enriched and cultured in PDA liquid medium at 30°C for 5-7d. Insert 15% by mass into the solid culture medium of tea leaves, and ferment at 30°C for 10-15 days;
[0024] (3) Preparation of tea solid medium: add 1000mg of boiling water to the fermented tea leaves, then add 180g of brown sugar, soak for 20min, and cool down;
[0025] (4) Yeast fermentation of tea leaves: Schizosaccharomyces kuri ( Schizosaccharomyces spombe CICC1360) was activated on wort solid medium, cultured on wort solid slant medium for 2-3 days, then inserted into wort liquid medium, and cultured at 28°C fo...
Embodiment 3
[0028] A method for producing tea wine by utilizing the saccharomyces coronoides, comprising the following steps:
[0029] (1) Preparation of tea solid culture medium: add 5mg of water to 50g of tea leaves, and sterilize under microwave 400w for 60s;
[0030] (2) Fungal fermentation of tea leaves: Physiocystis coronoides FBKL3.0002 ( Eurotium cristatus FBKL3.0002) was activated on PDA solid medium, enriched and cultured in PDA liquid medium at 30°C for 5-7d. Insert 15% by mass into the solid culture medium of tea leaves, and ferment at 30°C for 10-15 days;
[0031] (3) Preparation of tea solid culture medium: add 1000mg of boiling water to the fermented tea leaves, then add 200g of brown sugar, soak for 20min, and cool down;
[0032] (4) Yeast fermentation of tea leaves: Schizosaccharomyces kuri ( Schizosaccharomyces spombe CICC1360) was activated on wort solid medium, cultured on wort solid slant medium for 2-3 days, then inserted into wort liquid medium, and cultured at...
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