Micro-array chip based on double layer nano-structure substrate and preparation method thereof

A double-layer nanostructure and microarray chip technology, which is applied in nanostructure manufacturing, nanotechnology, nanotechnology, etc., to achieve the effect of less sample consumption, simple method and high sensitivity

Active Publication Date: 2017-12-19
上海格荣生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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Method used

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  • Micro-array chip based on double layer nano-structure substrate and preparation method thereof
  • Micro-array chip based on double layer nano-structure substrate and preparation method thereof
  • Micro-array chip based on double layer nano-structure substrate and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0056] Example 1 - DNA Microarray Chip

[0057] Step 1: Preparation of silicon sphere substrate by silicon sphere self-assembly method

[0058] 1) Stir 2.2mL of ammonia water and 26.5mL of ethanol at room temperature for 10min, add 0.75mL of tetraethyl orthosilicate and react for 3h at room temperature, then centrifuge and purify the reaction product at a speed of 9000rpm / min and disperse it into n-butyl In the alcohol solution, the silicon sphere solution is obtained.

[0059] 2) Add the obtained silicon sphere solution into water drop by drop, and the silicon spheres will spontaneously form a self-assembled monolayer on the surface of the water. When the clean glass slide was placed on the water surface, the formed silicon sphere monolayer spontaneously transferred to the glass slide surface, and after calcination at 500°C for 1 hour, a stable silicon sphere substrate was obtained. The silicon ball substrate has a three-dimensional spherical nanostructure.

[0060] Step 2...

example 2

[0078] Example 2 - sugar microarray chip

[0079] Step 1: Preparation of silicon sphere substrate by silicon sphere self-assembly method

[0080] 1) Stir 2.2mL of ammonia water and 26.5mL of ethanol at room temperature for 10min, add 0.75mL of tetraethyl orthosilicate and react for 3h at room temperature, then centrifuge and purify the reaction product at a speed of 9000rpm / min and disperse it into n-butyl In the alcohol solution, the silicon sphere solution is obtained.

[0081] 2) Add the obtained silicon sphere solution into water drop by drop, and the silicon spheres will spontaneously form a self-assembled monolayer on the surface of the water. When the clean glass slide was placed on the water surface, the formed silicon sphere monolayer spontaneously transferred to the glass slide surface, and after calcination at 500°C for 1 hour, a stable silicon sphere substrate was obtained. The silicon ball substrate has a three-dimensional spherical nanostructure.

[0082] Step 2...

example 3

[0100] Example 3 - Glycoprotein Microarray Chip

[0101] Step 1: Preparation of silicon sphere substrate by silicon sphere self-assembly method

[0102] 1) Stir 2.2mL of ammonia water and 26.5mL of ethanol at room temperature for 10min, add 0.75mL of tetraethyl orthosilicate and react for 3h at room temperature, then centrifuge and purify the reaction product at a speed of 9000rpm / min and disperse it into n-butyl In the alcohol solution, the silicon sphere solution is obtained.

[0103] 2) Add the obtained silicon sphere solution into water drop by drop, and the silicon spheres will spontaneously form a self-assembled monolayer on the surface of the water. When the clean glass slide was placed on the water surface, the formed silicon sphere monolayer spontaneously transferred to the glass slide surface, and after calcination at 500°C for 1 hour, a stable silicon sphere substrate was obtained. The silicon ball substrate has a three-dimensional spherical nanostructure.

[010...

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Abstract

The invention provides a micro-array chip based on a sphere-brush double layer nano-structure substrate and a preparation method thereof. The preparation method of the micro-array chip has versatility, and has the advantages of convenient process and less equipment requirement, and is suitable for large batch production; the micro-array chip can realize the analysis detection of interacting between nucleotide and nucleotide, sugar and protein, and protein and protein, and has the advantages of simpleness, less sample consumption and high sensitivity. The experiment result shows that the sugar micro-array chip prepared by the method has the detection limit on biotin-modified castor-oil plant agglutinin-120 and biotin-modified concanavalin agglutinin being 1 ng/mL, and the DNA micro-array chip has the detection limit on target DNA being 0.1 nmol/L; the glycoprotein micro-array chip has the detection limit on the biotin-modified castor-oil plant agglutinin-120 being 0.3 ng/ml, and the antibody micro-array chip has the detection limit on the CY5-modified rabbit-anti-human antibody being 10 pg/mL.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a ball-brush double-layer nanostructure substrate microarray chip and a preparation method thereof. Background technique [0002] Microarray chips are an important tool for genomics, proteomics, and glycomics research. Thousands of different microarrays are constructed on the surface of a solid-phase carrier on the order of square centimeters by robotic arm spotting technology or microelectronic lithography technology. Molecular arrays of probes to achieve accurate, rapid and informative detection of nucleic acids, proteins, sugars and other biological components, with the advantages of high throughput, automation, and small sample consumption (Nucleic Acids Res., 2014 , 43, D82–D86; Nat.Struct.Mol.Biol., 2015, 22(8), 603–610; Nat.Protoc., 2015, 10(5), 756–767). [0003] At present, biochips mainly use amino, aldehyde, epoxy or polylysine-modified flat glass as the carrier, but the tw...

Claims

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Application Information

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IPC IPC(8): B01L3/00B82B3/00G01N33/533G01N33/558
CPCB01L3/502707B01L2200/10B01L2300/0636B01L2300/0874B01L2300/0887B01L2300/0896B82B3/00G01N33/533G01N33/558
Inventor 王爱华刘霞王振新
Owner 上海格荣生物科技有限公司
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