30 results about "Antibody microarray" patented technology

The present invention is directed to a high throughput method for producing a large number of different antibodies, more specifically organized antibody microarrays. These antibodies and antibody microarrays can be used to rapidly assay protein abundance and identify types of proteins that are expressed in cells and tissues under a variety of conditions, or to compare protein expression profiles of different cells.

The invention discloses an on-site detection immuno-chip, comprising a chip carrier and an agarose gel layer which is paved on the chip carrier, wherein, the agarose gel layer is fixed with a plurality of antibody microarrays of 4*4, and chip-dedicated fences or Super PAP Pen scribings are used for separating the microarrays from each other; the double antibody sandwich principle is adopted to detect the antigen to carry out the dot matrix of corresponding capture antibody, positive control and negative control on the solid phase carrier simultaneously; the antibody protein is connected with the solid phase carrier through the covalent bond and physical adsorption; the sample liquid to be detected and the chip are directly incubated; the antigen to be detected in the samples combine with the corresponding antibody which is fixed on the chip; a specific monoclonal antibody probe marked by horse radish peroxidase is added and the macroscopic detection results can be obtained after the coloration of substrates. The invention can detect viruses in multiple aquatic animal samples and the results are macroscopic, thus being applicable to rapid and accurate detection of viruses of aquatic animals in breeding production.

The invention relates to a method and a device for detecting free epithelial cell organ sources in blood. The method includes the following steps: capturing epithelial cells from a blood sample; cracking the epithelial cells to release protein therein; specifically recognizing at least four protein markers with organ specificity; acquiring the organ sources of the epithelial cells by detecting expression quantity of the protein markers. The device comprises a microgroove chip and an antibody loading glass piece, 100-10000 microgrooves are formed in the microgroove chip and used for containing the cells, the antibody loading glass piece is used for loading an antibody micro-array which comprises at least four mutually-independent antibody strips, and the microgrooves and the antibody micro-array are arranged in a matched manner to enable at least one part of each antibody strip to be contained at corresponding positions of the microgrooves. The problem of detecting the epithelial cell organ sources in blood is solved by quickly and effectively detecting multiple proteins in single cells.

The invention discloses a chip and a bacterium analysis instrument for food safety detection by using impedance spectroscopy. The chip is based on a nanometer hole alumina film, modules with different bacterium antibody microarrays are formed on the chip after trusted platform module (TPM) modification, polyethylene glycol (PEG) layer formation and bacterium antibody microarray module formation, and the modules can be used for fast and sensitively detecting various kinds of bacteria in food at the same time. The bacterium analysis instrument disclosed by the invention has the characteristics that convenience and high speed are realized, the portability is good, the operation is simple, and the like, and the bacterium analysis instrument can be used for the field detection of the food safety.

The invention discloses an antibody chip kit for simultaneous quantitative detection of multiple receptors. The kit comprises antibody chips, a receptor standards mixture, a biotin labeled detection antibodies mixture and Cy3 fluorochrome labeled streptavidin. A standard tissue slide which is coated by reactive amino groups is chosen as a surface carrier of the antibody chips, and various antigen specific antibodies are adsorbed firmly on the surface of the slide by a non-contact spotting robot and sixteen microarrays are formed. The sixteen microarrays are then separated in non-interfering small areas by the use of a plastic frame with 2*8 holes and a latex closed frame which match each other and are demountable. Each capture antibody has four repeated spottings in each antibody microarray. The antibody chip kit can realize simultaneous quantitative detection of multiple receptors. Dynamic detection range of receptors is wider. Quadruple ELISA repeated data can be obtained from a single sample experiment. The antibody chip kit has advantages of high sensitivity, high throughput, small required use amount of samples, low cost, easy popularization and the like.

The invention discloses an immunity detection chip of prawn white spot syndrome virus (WSSV), comprising a chip carrier and an agarose gel layer which is paved on the chip carrier, wherein, the agarose gel layer is fixed with a plurality of antibody microarrays of 4*4, and chip-dedicated fences or Super PAP Pen scribings are used for separating the microarrays from each other. The invention comprises the following steps: adopting the sandwich method to detect the antigen; fixing the pathogenic polyclonal antibody (PcAb) on the chip slice base; taking the target organ tissue of the individual to be detected to prepare to-be-detected sample liquid; incubating directly the to-be-detected sample liquid with the chip which is fixed with PcAb to capture the antigen and lead the antigen to combine on the chip; adding a specific monoclonal antibody probe marked by fluorescence; and reading results through a CCD chip scanner. The invention has the advantage of detecting white spot syndrome virus (WSSV) in multiple samples simultaneously, and is applicable to the rapid and accurate detection of white spot syndrome virus (WSSV) of prawns / crabs in breeding production and the quarantine inspection of WSSV in import and export prawns / crabs.

An antibody microarray screen including a substrate, monoclonal and polyclonal antibodies that are purified immunoglobins, wherein the antibodies are spotted on predetermined positions on the substrate, and fluids unprocessed for immunoglobulin isolation (e.g., anti-sera, ascites fluids, or hybridoma culture media), wherein the unprocessed fluids are spotted on the predetermined positions on the substrate. Production of drug-metabolizing enzyme antibody microarrays containing closely related cytochromes P450 is disclosed. Methods of manufacturing an antibody microarray, an internal control molecule for use in an antibody microarray, a method of determining optimal spotting concentrations of IgG and a method to increase a detectable signal with microarray analysis are disclosed.

The invention discloses a fish lymphocystis disease virus (LCDV) immunity detection chip, and the structure of the chip comprises a chip carrier, an agarose gel layer covered on the chip carrier and a plurality of antibody microarrays fixed on the agarose gel layer; wherein, special fences for chips or Super PAP Pen rulings are arranged between the microarrays for separating the microarrays. In the invention, a sandwich method is adopted to detect antigen, pathogenic antibody is fixed on a film support of the chip, the to-be-detected sample solution is prepared by using virus target organ of a sample to be detected, the to-be-detected sample solution and the chip fixed with pathogenic antibody are directly incubated, a specific monoclonal antibody (McAb) probe with a fluorescent mark is added, and then a CCD scanner is used for reading the result. The invention has the advantages of detecting lymphocystis disease virus in various samples or different tissues in the same sample simultaneously, which can be used for rapid, sensitive, and accurate detection on LCDV in marine-culturing fishes and quarantine on LCDV in imported-exported fishes.

The invention discloses an antibody microarray kit for detecting the residue of aminoglycoside antibiotics in food. The antibody microarray kit comprises a microarray, an antibody, a secondary antibody marked by a Cy3, and an extraction reagent, wherein the antibody consists of a neomycin monoclonal antibody and a gentamycin monoclonal antibody, wherein the neomycin monoclonal antibody is secreted by a hybridoma cell EDC / 5G04 of which the preservation number is CCTCC NO:C201144; the gentamycin monoclonal antibody is secreted by a hybridoma cell EDC / 2D5 of which the preservation number is CCTCC NO:C201145, and the microarray fixes an amikacin coating antigen and a sisomicin coating antigen. The invention further discloses an antibody microarray method for detecting the residue of the aminoglycoside antibiotics in the food. The method can be used for detecting 5 aminoglycoside antibiotics at the same time, so that the method which is quick, is sensitive and is high in flux is provided for detecting the residue of the aminoglycoside antibiotics in the food, and the method has the characteristics of high accuracy, high precision, high efficiency and the like.

The invention provides a method for identifying heterophilic antibody interference in an antibody microarray system, which is mainly characterized in that: a heterophilic antibody identification point is increased during manufacturing an antibody microarray chip. The antibody of the identification point consists of analogues of solid phase antibodies of various indexes. The method comprises the following steps of: after samples with statistical significance quantity are detected, establishing judgment criteria of heterophilic antibody positive interference; and judging whether the later clinical samples of the same indexes have the heterophilic antibody positive interference according to the judgment criteria. The method identifies the heterophilic antibodies without increasing the conventional workload and reagents; the method does not need to add a blocking agent into each sample so as to save the consumption of many blocking agents, reduce the cost and reduce unknown interference; and meanwhile, automatic identification and objective judgment can be performed through a biochip reader, and analysis on the clinical symptoms of patients is not needed.

The present invention relates to a method for preparing sd Ab microarray comprising the step consisting of: —i) providing a host cell capable of expressing a biotinylation enzyme —ii) transforming said host cell with a nucleic acid encoding for a fusion protein wherein a single domain antibody is fused at its carboxy terminal end to a biotinylation peptide —iii) culturing said host cell in presence of biotin in such a way that said fusion protein and biotinylation enzyme are expressed, resulting in biotinylation of said fusion protein —iv) lysing said host cell as cultured at step iii) —v) spotting the lysate obtained at step iv) on a solid sup port coated with an agent selected from the group consisting of avidin, streptavidin and / or any art known derivative of these agents A further object of the invention relates to a sd Ab microarray obtainable by the method of the invention.

The present invention provides a monoclonal antibody recognizing modification after translation of p53 in a manner specific to a modification site, an antibody microarray comprising the antibody immobilized on a substrate, etc. Disclosed is a monoclonal antibody which reacts specifically with a peptide consisting of an amino acid sequence of at least 6 consecutive amino acids containing a predetermined amino acid residue of the amino acid sequence represented by SEQ ID NO: 1, wherein the amino acid residue is phosphorylated or acetylated, or with a peptide having one to several arbitrary amino acids added to the above peptide, but does not react with the above peptide which is not phosphorylated or acetylated.

The present invention provides a monoclonal antibody recognizing modification after translation of p53 in a manner specific to a modification site, an antibody microarray comprising the antibody immobilized on a substrate, etc. Disclosed is a monoclonal antibody which reacts specifically with a peptide consisting of an amino acid sequence of at least 6 consecutive amino acids containing a predetermined amino acid residue of the amino acid sequence represented by SEQ ID NO: 1, wherein the amino acid residue is phosphorylated or acetylated, or with a peptide having one to several arbitrary amino acids added to the above peptide, but does not react with the above peptide which is not phosphorylated or acetylated.

The invention provides a method for detecting one or more biological ligands, where the method generally uses two arrays of biological reagents. The two arrays have two different functionalities: the first array captures the ligands on the array; and the second array delivers detecting reagents to the captured ligands. Use of arrays affords the method high-throughput detection capability; and the use of two different arrays ensures high specificity. The first array has a support structure fixed with a first set of reagents, each at a pre-determined position on the support. The second array has a support structure fixed with a second set of reagents, each at a pre-determined position on the support. The first array contains reagent(s) binding to a ligand, while the second array contains second reagent(s) binding to the same ligand. In preferred embodiments, antibodies are used as reagents, and used in detecting proteins in protein samples.

The invention discloses a fish lymphocystis disease virus (LCDV) immunity detection chip, and the structure of the chip comprises a chip carrier, an agarose gel layer covered on the chip carrier and a plurality of antibody microarrays fixed on the agarose gel layer; wherein, special fences for chips or Super PAP Pen rulings are arranged between the microarrays for separating the microarrays. In the invention, a sandwich method is adopted to detect antigen, pathogenic antibody is fixed on a film support of the chip, the to-be-detected sample solution is prepared by using virus target organ of a sample to be detected, the to-be-detected sample solution and the chip fixed with pathogenic antibody are directly incubated, a specific monoclonal antibody (McAb) probe with a fluorescent mark is added, and then a CCD scanner is used for reading the result. The invention has the advantages of detecting lymphocystis disease virus in various samples or different tissues in the same sample simultaneously, which can be used for rapid, sensitive, and accurate detection on LCDV in marine-culturing fishes and quarantine on LCDV in imported-exported fishes.

A method for setting-up antibody micro-array based on double-antibody sandwich immunological analysis includes preparing carrier, activating carrier, fixing point sample of catching antibody and immunological analysis based on micro-array.

The invention provides a micro-array chip based on a sphere-brush double layer nano-structure substrate and a preparation method thereof. The preparation method of the micro-array chip has versatility, and has the advantages of convenient process and less equipment requirement, and is suitable for large batch production; the micro-array chip can realize the analysis detection of interacting between nucleotide and nucleotide, sugar and protein, and protein and protein, and has the advantages of simpleness, less sample consumption and high sensitivity. The experiment result shows that the sugar micro-array chip prepared by the method has the detection limit on biotin-modified castor-oil plant agglutinin-120 and biotin-modified concanavalin agglutinin being 1 ng / mL, and the DNA micro-array chip has the detection limit on target DNA being 0.1 nmol / L; the glycoprotein micro-array chip has the detection limit on the biotin-modified castor-oil plant agglutinin-120 being 0.3 ng / ml, and the antibody micro-array chip has the detection limit on the CY5-modified rabbit-anti-human antibody being 10 pg / mL.

A method for constructing an antibody microarray based on a double-antibody sandwich immunoassay, characterized in that the construction steps include: preparation of a carrier; activation of the carrier; spotting and fixing of a capture antibody; and immunoassay based on the microarray. The antibody microarray constructed according to this optimized operation procedure has good reproducibility (intra-group and inter-group variation <10%) and quantitative analysis ability (standard curve correlation between antigen concentration and signal intensity>0.95 ), providing a reliable technical platform for parallelism and multi-factor protein quantitative detection. In addition, the chip is stable after spotting, and the activity can be maintained for at least 2 months, which provides important conditions for the industrialization and scale of the chip.

A genetic polymorphism associated with myocardial infarction is provided. More particularly, provided are a polynucleotide including a single nucleotide polymorphism (SNP) or a haplotype associated with myocardial infarction, a polynucleotide hybridized with the polynucleotide, a polypeptide encoded by one of the polynucleotides, an antibody bound to the polypeptide, a microarray and a kit including one of the polynucleotides, a myocardial infarction diagnosis method, a SNP detecting method and a method of screening pharmaceutical compositions for myocardial infarction.

The invention discloses an active chemiluminescent protein chip which comprises a reaction cup, a locking ring, a base and a porous carrier, the porous carrier is coated with an antigen or antibody microarray, an annular tightening part is coaxially arranged at the bottom of the reaction cup, and a plurality of elastic tightening pieces are uniformly distributed on the circumference of the bottom of the annular tightening part; the cup body of the reaction cup is sleeved with the locking ring, and an annular clamping groove is formed in the lower portion of the inner side of the locking ring; the base is of a hollow structure and is vertically through, and the opening end of the bottom of the base is connected with an external pump structure. According to the invention, after a common percolation type color development type protein chip is improved into the active chemiluminescence protein chip, the infiltration time and rate of liquid can be controlled autonomously and accurately, the sensitivity of product detection is improved, and the design and manufacturing requirements of products with higher sensitivity requirements are met.

The invention discloses an antibody microarray kit for detecting the residue of aminoglycoside antibiotics in food. The antibody microarray kit comprises a microarray, an antibody, a secondary antibody marked by a Cy3, and an extraction reagent, wherein the antibody consists of a neomycin monoclonal antibody and a gentamycin monoclonal antibody, wherein the neomycin monoclonal antibody is secreted by a hybridoma cell EDC / 5G04 of which the preservation number is CCTCC NO:C201144; the gentamycin monoclonal antibody is secreted by a hybridoma cell EDC / 2D5 of which the preservation number is CCTCC NO:C201145, and the microarray fixes an amikacin coating antigen and a sisomicin coating antigen. The invention further discloses an antibody microarray method for detecting the residue of the aminoglycoside antibiotics in the food. The method can be used for detecting 5 aminoglycoside antibiotics at the same time, so that the method which is quick, is sensitive and is high in flux is provided for detecting the residue of the aminoglycoside antibiotics in the food, and the method has the characteristics of high accuracy, high precision, high efficiency and the like.

The invention discloses an antibody chip kit for simultaneous quantitative detection of multiple receptors. The kit comprises antibody chips, a receptor standards mixture, a biotin labeled detection antibodies mixture and Cy3 fluorochrome labeled streptavidin. A standard tissue slide which is coated by reactive amino groups is chosen as a surface carrier of the antibody chips, and various antigen specific antibodies are adsorbed firmly on the surface of the slide by a non-contact spotting robot and sixteen microarrays are formed. The sixteen microarrays are then separated in non-interfering small areas by the use of a plastic frame with 2*8 holes and a latex closed frame which match each other and are demountable. Each capture antibody has four repeated spottings in each antibody microarray. The antibody chip kit can realize simultaneous quantitative detection of multiple receptors. Dynamic detection range of receptors is wider. Quadruple ELISA repeated data can be obtained from a single sample experiment. The antibody chip kit has advantages of high sensitivity, high throughput, small required use amount of samples, low cost, easy popularization and the like.

The invention discloses an immunity detection chip of prawn white spot syndrome virus (WSSV), comprising a chip carrier and an agarose gel layer which is paved on the chip carrier, wherein, the agarose gel layer is fixed with a plurality of antibody microarrays of 4*4, and chip-dedicated fences or Super PAP Pen scribings are used for separating the microarrays from each other. The invention comprises the following steps: adopting the sandwich method to detect the antigen; fixing the pathogenic polyclonal antibody (PcAb) on the chip slice base; taking the target organ tissue of the individual to be detected to prepare to-be-detected sample liquid; incubating directly the to-be-detected sample liquid with the chip which is fixed with PcAb to capture the antigen and lead the antigen to combine on the chip; adding a specific monoclonal antibody probe marked by fluorescence; and reading results through a CCD chip scanner. The invention has the advantage of detecting white spot syndrome virus (WSSV) in multiple samples simultaneously, and is applicable to the rapid and accurate detection of white spot syndrome virus (WSSV) of prawns / crabs in breeding production and the quarantine inspection of WSSV in import and export prawns / crabs.

The invention provides a micro-array chip based on a sphere-brush double layer nano-structure substrate and a preparation method thereof. The preparation method of the micro-array chip has versatility, and has the advantages of convenient process and less equipment requirement, and is suitable for large batch production; the micro-array chip can realize the analysis detection of interacting between nucleotide and nucleotide, sugar and protein, and protein and protein, and has the advantages of simpleness, less sample consumption and high sensitivity. The experiment result shows that the sugar micro-array chip prepared by the method has the detection limit on biotin-modified castor-oil plant agglutinin-120 and biotin-modified concanavalin agglutinin being 1 ng / mL, and the DNA micro-array chip has the detection limit on target DNA being 0.1 nmol / L; the glycoprotein micro-array chip has the detection limit on the biotin-modified castor-oil plant agglutinin-120 being 0.3 ng / ml, and the antibody micro-array chip has the detection limit on the CY5-modified rabbit-anti-human antibody being 10 pg / mL.

A nanoarray or microarray of antibodies includes a planar surface having an ultraflat surface, which has a planar substrate functionalized with a first monolayer of linking molecules, preferably APTES molecules, and a second monolayer of small peptides of the general formula (I) capable of binding the Fc region of antibodies. The nanoarray or microarray includes capture antibodies immobilized on the planar support, preferably in the form of a matrix of spots, and is suitable to be used for detecting antigens in a biological sample by AFM imaging.Z—HN—R1—R2—R3—R4—R5—R6—NH—X—CO-G-COOH.

The invention discloses a rotary chemiluminescent protein chip, which comprises a rotor, a reaction ring, a base and a porous carrier coated with an antigen or antibody microarray, the porous carrier is pasted on the peripheral ring surface of the rotor, the rotor is arranged in a reaction cup, a magnet is arranged in the rotor, a circle of ring edge is arranged on the periphery of the lower part of the rotor, and the ring edge is arranged on the base. The annular edge is connected with the rotor through a connecting rod at intervals, and the rotor and the annular edge define a vertically-through circular flow guide groove; a vertically through liquid leading-out hole is formed in the bottom surface of the reaction cup, and the reaction cup is arranged in the base; a drain pipe is arranged in a bottom panel of the base, one end of the drain pipe is communicated with the inside of the base, and the other end of the drain pipe is connected with a negative pressure pump. By improving the chip structure and under the assistance of magnetic stirring and pump suction, the detection repeatability can be improved by assisting high-efficiency reaction of each step, various liquids used in the detection process can be timely and orderly guided out, and the design and manufacturing requirements of products with higher repeatability requirements can be met.

The invention relates to a method and a device for detecting free epithelial cell organ sources in blood. The method includes the following steps: capturing epithelial cells from a blood sample; cracking the epithelial cells to release protein therein; specifically recognizing at least four protein markers with organ specificity; acquiring the organ sources of the epithelial cells by detecting expression quantity of the protein markers. The device comprises a microgroove chip and an antibody loading glass piece, 100-10000 microgrooves are formed in the microgroove chip and used for containing the cells, the antibody loading glass piece is used for loading an antibody micro-array which comprises at least four mutually-independent antibody strips, and the microgrooves and the antibody micro-array are arranged in a matched manner to enable at least one part of each antibody strip to be contained at corresponding positions of the microgrooves. The problem of detecting the epithelial cell organ sources in blood is solved by quickly and effectively detecting multiple proteins in single cells.

The invention discloses an improved antibody chip kit capable of quantitatively detecting multiple cell factors synchronously. The kit includes a standard tissue slide which is coated with activated amino groups and is used as a surface carrier. Various antigen-specificity antibodies are spotted on a surface of the slide to form a microarray through a non-contacting sample spotting instrument. The slide is divided into sixteen small zones, which are not interfered with each other, through a detachable 2*8-hole plastic frame, wherein each small zone contains an antibody microarray. Each captured antibody is repeated by four times in each antibody microarray. The slide and the frame are tightly clamped with each other through plastic clamping plates which are arranged at the two sides to form an antibody chip. Experimental reagents of a multiple sandwich ELISA reaction are completed on the surface of the slide. By means of the antibody chip kit, multiple cell factors can be quantitatively detected synchronously. A sensitivity of the kit is similar to that of single ELISA but a dynamic detection range of the cell factors is wider. Repeated data, which is as four times as that of ELISA, can be obtained from a single-sample experiment. The kit is high in sensitivity, is high in flux, is little in sample usage amount, is low in cost and is easy to popularize.