The invention discloses a rotary chemiluminescent protein chip, which comprises a rotor, a reaction ring, a base and a porous carrier coated with an antigen or antibody microarray, the porous carrier is pasted on the peripheral ring surface of the rotor, the rotor is arranged in a reaction cup, a magnet is arranged in the rotor, a circle of ring edge is arranged on the periphery of the lower part of the rotor, and the ring edge is arranged on the base. The annular edge is connected with the rotor through a connecting rod at intervals, and the rotor and the annular edge define a vertically-through circular flow guide groove; a vertically through liquid leading-out hole is formed in the bottom surface of the reaction cup, and the reaction cup is arranged in the base; a drain pipe is arranged in a bottom panel of the base, one end of the drain pipe is communicated with the inside of the base, and the other end of the drain pipe is connected with a negative pressure pump. By improving the chip structure and under the assistance of magnetic stirring and pump suction, the detection repeatability can be improved by assisting high-efficiency reaction of each step, various liquids used in the detection process can be timely and orderly guided out, and the design and manufacturing requirements of products with higher repeatability requirements can be met.