An improved antibody chip kit for simultaneous quantitative detection of multiple cytokines

Abstract

The invention discloses an improved antibody chip kit capable of quantitatively detecting multiple cell factors synchronously. The kit includes a standard tissue slide which is coated with activated amino groups and is used as a surface carrier. Various antigen-specificity antibodies are spotted on a surface of the slide to form a microarray through a non-contacting sample spotting instrument. The slide is divided into sixteen small zones, which are not interfered with each other, through a detachable 2*8-hole plastic frame, wherein each small zone contains an antibody microarray. Each captured antibody is repeated by four times in each antibody microarray. The slide and the frame are tightly clamped with each other through plastic clamping plates which are arranged at the two sides to form an antibody chip. Experimental reagents of a multiple sandwich ELISA reaction are completed on the surface of the slide. By means of the antibody chip kit, multiple cell factors can be quantitatively detected synchronously. A sensitivity of the kit is similar to that of single ELISA but a dynamic detection range of the cell factors is wider. Repeated data, which is as four times as that of ELISA, can be obtained from a single-sample experiment. The kit is high in sensitivity, is high in flux, is little in sample usage amount, is low in cost and is easy to popularize.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to an antibody chip kit for quantitatively detecting multiple cytokines simultaneously. technical background [0002] Cytokines are small-molecule polypeptides (low-molecular-weight proteins) synthesized and secreted by immune cells and non-immune cells of the body that regulate various cellular physiological functions. [0003] Cytokines have a very wide range of biological activities, including promoting the proliferation and differentiation of target cells, enhancing anti-infection and cell killing effects, promoting or inhibiting the expression of other cytokines and membrane surface molecules, promoting inflammatory processes, and affecting cell metabolism. Immune-related cytokines mainly include lymphokines produced by lymphocytes, monokine produced by mononuclear macrophages, interleukin (IL), interferon (IFN), colony stimulating factor (CSF) ), tumor necrosis factor (TNF)...

AI Technical Summary

Problems solved by technology

[0009] Aiming at the deficiencies in the prior art, the object of the present invention is to provide an improved body chip kit for quantitatively detecting multiple cytokines at the same time. The kit uses fluorescent detection signals to quantitatively detect dozens of cytokines simultaneously, overcoming the The existing technology has defects such as cumbersome operation, single detection index, and low sensitivity. It has the advantages of low cost, convenience, high throughput, high sensitivity, high specificity, less specimen consumption, and can be popularized and scaled in ordinary laboratories.

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