32results about How to "Less specimen" patented technology

The invention relates to an antibody chip kit for diagnosis of various tumors. The kit includes an antibody chip, a tumor marker standard substance mixture, a biotin-labeled tumor marker detection antibody mixture, and a fluorescein Cy3-labeled streptavidin, wherein the antibody chip includes a substrate, 16 kinds of tumor marker specific antibodies fixed on the surface of the substrate, and two kinds of positive controls, and the tumor marker standard substance mixture is a freeze-dried mixture obtained by mixing 16 kinds of standard tumor marker standard substances together according to a certain amount. The kit can detect 16 clinical commonly used tumor markers, overcomes the defects of complicated operation, single detection index, low sensitivity and the like in the prior art, has the advantages of being cheap, convenient, sensitive, accurate, high in throughput, and less in amount of samples, and can be popularized and large-scaled in common laboratories.

The invention discloses an antibody chip kit for screening biomarkers. The kit comprises an antibody chip, a biotin labeled cell factor detection antibody mixture and horse radish peroxidase labeled streptavidin, wherein the antibody chip comprises a bas membrane and specific antibodies of 60 cell factors fixed on the surface of the base membrane, three positive control. The kit disclosed by the invention can be used for simultaneously detecting 60 cell factors for multiple samples, overcomes the defects in the prior art of tedious operation, single detection index, low sensitivity and the like and has the advantages that the kit is cheap, convenient, sensitive, accurate, high in throughput and few in use level of specimens and can be popularized and expanded on a common laboratory and the like.

The invention discloses a protein chip and a kit for abortive tuberculosis diagnosis. The protein chip contains a specific antibody for 29 proteins. The protein chip can specifically separate an abortive tuberculosis patient from healthy people, nontuberculous pneumonia patients and the healed tuberculosis patients, has relatively high sensitivity and specificity, high flux and less consumption of specimens and is convenient in popularization and mass production in ordinary laboratories; and meanwhile, the application in the clinical diagnosis is favorable for more rapidly and accurately discovering the abortive tuberculosis patient and is expected to be used for screening the abortive tuberculosis and diagnosing the abortive tuberculosis, inspecting the healthy people, predicting and evaluating the curative effect of the tuberculosis treatment and the like, and provides strong technical support for controlling the situation of the tuberculosis.

The invention relates to an antibody chip for joint detection of leukemia markers, wherein the leukemia markers are selected from: ENA-78, GCP-2, MDC, MIF, MIP-3a, MPIF-1, MSPa, OPN, GDF-15, HB-EGF , HGF, PDGF‑AA, VEGF‑D, BLC, G‑CSF, TNF‑RⅠ, uPAR, TNFa, Fas L, IL4, ICAM‑1, IL5, KC, TIMP‑1, TARC, IL2, IL7, M‑ At least 17 of CSF, IL10, IL21, IL‑12p70, BDNF, PF‑4, EGF, TNFRⅡ, and LIGHT can simultaneously measure the expression levels of multiple samples and multiple leukemia markers to accurately and comprehensively reflect the occurrence and course of leukemia , to improve its diagnosis, disease course monitoring and prognosis assessment.

Provided is a phosphorylation antibody-array test kit for detecting receptor tyrosine kinase (RTK), comprising: a solid-phase support, which is a standard plate coated with specific antibodies; a washing liquid comprising 20X concentrated washing liquid containing 0.1% Tween 20 and dilution thereof; a sample dilution; a dilution used for diluting antibodies and HRP-streptavidin; a biotinylated detection antibody mixture; a solution of 300X concentrated fluorescein-streptavidin; a sample processing liquid, which is 2X cell lysate; the specific antibody is an antibody for 71 types of protein. The RTK phosphorylation antibody-array test kit is capable of quickly, simply, and accurately testing by means of a single experiment for the phosphorylation conditions of 71 proteins in an RTK pathway; By means of monitoring changes in protein phosphorylation in an experimental model system, pathway activation in cells may be rapidly detected, and the tedious processes of immunoprecipitation and blotting are unnecessary.

The invention provides a homogeneous luminescence immunoassay method for quantitatively analyzing multiple components simultaneously and a kit used for the method. Receptor microspheres containing various different fluoresceins are adopted, and antibody molecules for capturing different biological markers to be measured are enveloped in the method; in a measuring process, the multiple biological markers in a sample to be measured are combined with the corresponding antibody molecules on the surfaces of the receptor microspheres and biotinylated antibodies in a detection system respectively to form double-antibody sandwich compositions which are respectively connected with donor microspheres labeled with streptavidin; when the donor microspheres are irradiated by exciting light, all types of the receptor microspheres send out optical signals with different wavelengths; the intensities of the light with different wavelengths are respectively detected, so that the biological markers to be measured can be accurately quantified. The kit comprises the receptor microspheres containing chemiluminescence reagents and the fluoresceins, the biotinylated antibodies and the donor microspheres containing photosensitive substances. The homogeneous luminescence immunoassay method and the kit have the beneficial effects that simultaneous and quantitative measurement on multiple components is realized, and the detection cost is reduced.

Provided is an antibody-array test kit for detecting proteins related to periodontal disease, comprising an antibody array; said antibody array uses as its solid-phase support a glass slide treated with a hydrophilic reagent containing an alkyl glycoside, and a specific antibody mixture of various periodontal disease-related proteins is spotted onto the solid-phase support under conditions of 22-24°C and 30-40% humidity. The test kit is capable of detecting a plurality of proteins related to periodontal disease and commonly used for clinical purposes, and has the advantages that it is sensitive, high-throughput, uses a small amount of specimen, and may be popularized and used on a large scale in conventional laboratories.