32results about How to "Less specimen" patented technology

The invention relates to an antibody chip kit for diagnosis of various tumors. The kit includes an antibody chip, a tumor marker standard substance mixture, a biotin-labeled tumor marker detection antibody mixture, and a fluorescein Cy3-labeled streptavidin, wherein the antibody chip includes a substrate, 16 kinds of tumor marker specific antibodies fixed on the surface of the substrate, and two kinds of positive controls, and the tumor marker standard substance mixture is a freeze-dried mixture obtained by mixing 16 kinds of standard tumor marker standard substances together according to a certain amount. The kit can detect 16 clinical commonly used tumor markers, overcomes the defects of complicated operation, single detection index, low sensitivity and the like in the prior art, has the advantages of being cheap, convenient, sensitive, accurate, high in throughput, and less in amount of samples, and can be popularized and large-scaled in common laboratories.

The invention belongs to the technical field of biomedicine, and particularly relates to a gastric cancer detection kit adopting HOXB9 and PBX1 as biomarkers and an application. With the adoption of the detection kit, HOXB9 and PBX1 in a tissue sample are detected jointly, the gastric cancer is diagnosed and detected, and detection results have good sensitivity and high specificity. The kit can be effectively used for early screening and / or prognosis evaluation of the gastric cancer and has good clinical application prospect.

The invention discloses an antibody chip kit for screening biomarkers. The kit comprises an antibody chip, a biotin labeled cell factor detection antibody mixture and horse radish peroxidase labeled streptavidin, wherein the antibody chip comprises a bas membrane and specific antibodies of 60 cell factors fixed on the surface of the base membrane, three positive control. The kit disclosed by the invention can be used for simultaneously detecting 60 cell factors for multiple samples, overcomes the defects in the prior art of tedious operation, single detection index, low sensitivity and the like and has the advantages that the kit is cheap, convenient, sensitive, accurate, high in throughput and few in use level of specimens and can be popularized and expanded on a common laboratory and the like.

The invention relates to an antibody chip kit for early diagnosis of acute kidney injury. The kit comprises an antibody chip, an acute kidney injury marker standard substance mixture, a biotin labeled acute kidney injury marker standard substance detection antibody mixture and fluorescein Cy3 labeled streptavidin, wherein the antibody chip comprises a standard structure slide and specific antibodies of 20 acute kidney injury markers fixed on the surface of the slide, three positive control; the acute kidney injury marker standard substance detection antibody mixture is a freeze-drying mixture which is prepared by mixing the 20 acute kidney injury marker standard substances at a certain dose. The kit provided by the invention can be used for detecting 20 clinically common acute kidney injury markers, overcomes the defects in the prior art of tedious operation, single detection index, low sensitivity and the like and has the advantages that the kit is cheap, convenient, sensitive, accurate, high in throughput and few in use level of specimens and can be popularized and expanded on a common laboratory and the like.

The invention discloses a protein chip and a kit for abortive tuberculosis diagnosis. The protein chip contains a specific antibody for 29 proteins. The protein chip can specifically separate an abortive tuberculosis patient from healthy people, nontuberculous pneumonia patients and the healed tuberculosis patients, has relatively high sensitivity and specificity, high flux and less consumption of specimens and is convenient in popularization and mass production in ordinary laboratories; and meanwhile, the application in the clinical diagnosis is favorable for more rapidly and accurately discovering the abortive tuberculosis patient and is expected to be used for screening the abortive tuberculosis and diagnosing the abortive tuberculosis, inspecting the healthy people, predicting and evaluating the curative effect of the tuberculosis treatment and the like, and provides strong technical support for controlling the situation of the tuberculosis.

The invention relates to an application of a two-dimensional graphdiyne material (with single layer or a plurality of layers) for a non-diagnosis purpose of biomarker fluorescence detection. Specifically, a biomarker includes a disease biomarker small-molecular compound, DNA, a nucleic acid aptamer, a protein, a peptide chain or other biological molecules. Through adopting of the two-dimensional graphdiyne material, the detection sensitivity can be substantially increased and the detection time is shortened at the same time, the use amount of specimens is reduced, the cost of testing is reduced, real-time detection of a variety of biological molecules can be realized simultaneously, and the two-dimensional graphdiyne material has broad application prospects for epidemic prevention of epidemic diseases such as influenza.

The invention relates to an antibody chip for joint detection of leukemia markers, wherein the leukemia markers are selected from: ENA-78, GCP-2, MDC, MIF, MIP-3a, MPIF-1, MSPa, OPN, GDF-15, HB-EGF , HGF, PDGF‑AA, VEGF‑D, BLC, G‑CSF, TNF‑RⅠ, uPAR, TNFa, Fas L, IL4, ICAM‑1, IL5, KC, TIMP‑1, TARC, IL2, IL7, M‑ At least 17 of CSF, IL10, IL21, IL‑12p70, BDNF, PF‑4, EGF, TNFRⅡ, and LIGHT can simultaneously measure the expression levels of multiple samples and multiple leukemia markers to accurately and comprehensively reflect the occurrence and course of leukemia , to improve its diagnosis, disease course monitoring and prognosis assessment.

The invention relates to an establishing method of a serum diagnosing model of active tuberculosis, which has the following steps: step 1 is a serum source taking and preparing step which comprises an experiment group and a control group, the venous blood of a checked person is collected, serum and the rest are separately stored after serum separation at the temperature of -80 DEG C in a refrigerator for spare use; step 2 is the step of the mass spectrometric analysis of the serum and comprises sample treatment, protein plate check, spectrometric analysis and data acquisition; step 3 is the step of statistic analysis and the difference protein screening; step 4 is the step of establishing the diagnosing model, and Biomarker Pattern 5.0 software is used for establishing the model of diagnosing active tuberculosis. When the model is used for identifying the active tuberculosis and the control group, the sensitivity is 96.9 percent, the specificity is 97.8 percent, the positive predicating value and the negative predicating value are 97.7 percent and 97.1 percent, and the total accurate rate is 97.3 percent.

The invention discloses a kit for detecting phosphorylated epidermal growth factor receptor and the kit adopts the protein chip technology. The kit comprises a base film (1) fixed with several kinds of specific antibodies and reactant and detection agent (2), wherein specific antibodies and phosphorylated epidermal growth factor receptor can perform antibody-antigen reaction, each kind of specific antibodies are fixed on a plurality of independent recognition sites which are fixed on the base film; and the reactant and detection agent (2) are used to detect whether the substance which can perform antibody-antigen reaction with specific antibodies exists in a sample to be detected through the array-ELISA method. The invention also discloses a preparation method of the kit. By adopting the kit of the invention, 17 phosphorylated epidermal growth factor receptors in a plurality of samples can be detected, lots of defects of the prior art can be overcome and the kit has the advantages that the kit is cheap, convenient, sensitive and accurate, has high flux and less amount of sample and can be popularized in the ordinary laboratory and scaled, etc.

Provided is a phosphorylation antibody-array test kit for detecting receptor tyrosine kinase (RTK), comprising: a solid-phase support, which is a standard plate coated with specific antibodies; a washing liquid comprising 20X concentrated washing liquid containing 0.1% Tween 20 and dilution thereof; a sample dilution; a dilution used for diluting antibodies and HRP-streptavidin; a biotinylated detection antibody mixture; a solution of 300X concentrated fluorescein-streptavidin; a sample processing liquid, which is 2X cell lysate; the specific antibody is an antibody for 71 types of protein. The RTK phosphorylation antibody-array test kit is capable of quickly, simply, and accurately testing by means of a single experiment for the phosphorylation conditions of 71 proteins in an RTK pathway; By means of monitoring changes in protein phosphorylation in an experimental model system, pathway activation in cells may be rapidly detected, and the tedious processes of immunoprecipitation and blotting are unnecessary.

The invention provides a homogeneous luminescence immunoassay method for quantitatively analyzing multiple components simultaneously and a kit used for the method. Receptor microspheres containing various different fluoresceins are adopted, and antibody molecules for capturing different biological markers to be measured are enveloped in the method; in a measuring process, the multiple biological markers in a sample to be measured are combined with the corresponding antibody molecules on the surfaces of the receptor microspheres and biotinylated antibodies in a detection system respectively to form double-antibody sandwich compositions which are respectively connected with donor microspheres labeled with streptavidin; when the donor microspheres are irradiated by exciting light, all types of the receptor microspheres send out optical signals with different wavelengths; the intensities of the light with different wavelengths are respectively detected, so that the biological markers to be measured can be accurately quantified. The kit comprises the receptor microspheres containing chemiluminescence reagents and the fluoresceins, the biotinylated antibodies and the donor microspheres containing photosensitive substances. The homogeneous luminescence immunoassay method and the kit have the beneficial effects that simultaneous and quantitative measurement on multiple components is realized, and the detection cost is reduced.

Provided is an antibody-array test kit for detecting proteins related to periodontal disease, comprising an antibody array; said antibody array uses as its solid-phase support a glass slide treated with a hydrophilic reagent containing an alkyl glycoside, and a specific antibody mixture of various periodontal disease-related proteins is spotted onto the solid-phase support under conditions of 22-24°C and 30-40% humidity. The test kit is capable of detecting a plurality of proteins related to periodontal disease and commonly used for clinical purposes, and has the advantages that it is sensitive, high-throughput, uses a small amount of specimen, and may be popularized and used on a large scale in conventional laboratories.

The invention relates to the technical fields of reproductive medicine and immune application, in particular to an antigen for detecting sperm antibodies, an expression carrier thereof, a kit and a detection method thereof. An antigen used for detecting sperm antibodies of the present invention is a polypeptide encoded by a gene fragment of the PB functional region of the recombinant IZUMO gene, and its sequence has the sequence shown in SEQ ID NO:1. The antigen used to detect sperm antibody is the key protein of sperm acrosome reaction, which determines the success of fertilization, and the antibody against this antigen is one of the important causes of immune infertility. The ELISA kit for sperm antibody detection can detect anti-sperm antibodies, overcomes the existing defects of low sensitivity and low specificity, and is convenient, sensitive, requires less specimen, can be tested in large quantities, and can be popularized and scaled in ordinary laboratories Etc. Therefore, the present invention is expected to be used in the screening and diagnosis of antisperm antibodies in infertile patients.