32results about How to "Less specimen" patented technology

The invention relates to an antibody chip kit for diagnosis of various tumors. The kit includes an antibody chip, a tumor marker standard substance mixture, a biotin-labeled tumor marker detection antibody mixture, and a fluorescein Cy3-labeled streptavidin, wherein the antibody chip includes a substrate, 16 kinds of tumor marker specific antibodies fixed on the surface of the substrate, and two kinds of positive controls, and the tumor marker standard substance mixture is a freeze-dried mixture obtained by mixing 16 kinds of standard tumor marker standard substances together according to a certain amount. The kit can detect 16 clinical commonly used tumor markers, overcomes the defects of complicated operation, single detection index, low sensitivity and the like in the prior art, has the advantages of being cheap, convenient, sensitive, accurate, high in throughput, and less in amount of samples, and can be popularized and large-scaled in common laboratories.

The invention discloses an antibody chip kit for simultaneous quantitative detection of multiple receptors. The kit comprises antibody chips, a receptor standards mixture, a biotin labeled detection antibodies mixture and Cy3 fluorochrome labeled streptavidin. A standard tissue slide which is coated by reactive amino groups is chosen as a surface carrier of the antibody chips, and various antigen specific antibodies are adsorbed firmly on the surface of the slide by a non-contact spotting robot and sixteen microarrays are formed. The sixteen microarrays are then separated in non-interfering small areas by the use of a plastic frame with 2*8 holes and a latex closed frame which match each other and are demountable. Each capture antibody has four repeated spottings in each antibody microarray. The antibody chip kit can realize simultaneous quantitative detection of multiple receptors. Dynamic detection range of receptors is wider. Quadruple ELISA repeated data can be obtained from a single sample experiment. The antibody chip kit has advantages of high sensitivity, high throughput, small required use amount of samples, low cost, easy popularization and the like.

The present invention relates to the technical field of biological detection, particular to an antibody chip kit for detecting periodontal disease related proteins. According to the antibody chip kit, a glass slide treated with a hydrophilic reagent containing alkyl glycoside as a solid-phase carrier, and the specific antibody mixture of a variety of periodontal disease related protein is spotted onto the solid-phase carrier at a temperature of 22-24 DEG C under humidity of 30-40%. According to the present invention, with the kit, a variety of the periodontal disease related protein commonly used in clinic can be detected, the defects of complex operation, single detection index, low sensitivity and the like in the prior art are overcome, and the kit has advantages of low cost, convenience, high sensitivity, accuracy, high throughput, less sample consumption, promotion and scalization in general laboratories, and the like.

The present invention relates to the technical field of biological detection, particularly to an antibody chip kit for detecting cell adhesion molecules. According to the antibody chip kit, a glass slide treated with a hydrophilic reagent containing alkyl glycoside as a solid-phase carrier, and the specific antibody mixture of a variety of cell adhesion factors is spotted onto the solid-phase carrier at a temperature of 20-26 DEG C under humidity of 35-45%. According to the present invention, with the kit, a variety of the cell adhesion factors commonly used in clinic can be detected, the defects of complex operation, single detection index, low sensitivity and the like in the prior art are overcome, and the kit has advantages of low cost, convenience, high sensitivity, accuracy, high throughput, less sample consumption, promotion and scalization in general laboratories, and the like.

The invention belongs to the technical field of biological medicines and particularly relates to a biological marker group for optic neuromyelitis spectrum diseases, application of the biological marker group, a protein chip and a kit. The invention provides an optic neuromyelitis spectrum disease biomarker group which is characterized by comprising monocyte chemotactic protein-3, LIGHT, macrophage inflammatory protein-1 delta, insulin-like growth factor-2, a glucocorticoid induced tumor necrosis factor receptor, thrombopoietin and a herpes virus entry medium, wherein the LIGHT is a lymphotoxin analogue which can be induced and expressed on a T cell and competes with glycoprotein D of HSV to be combined with HVEM. The application fills the blank that no reliable and accurate product and method for diagnosing and identifying optic neuromyelitis lineage diseases exist clinically at present.

The invention relates to the field of biological science, and provides a chip technology for detecting colorectal cancer related antibodies and an antibody chip detection kit. An antibody chip comprises a substrate-based solid phase antibody chip and a microbead or microgel-based liquid suspension chip. A protein chip technology is used to fix specific antibodies related to colorectal cancer, andmultiple antigenic proteins related to the colorectal cancer in a sample can be simultaneously detected. The kit includes the antibody chip, a standard product, a sample diluent, a blocking solution,a labeling solution, a detecting solution, and a washing solution. Through the comprehensive calculation of several detection indicators, an auxiliary means for the detection and analysis of the colorectal cancer related antibodies is provided. Compared with a kit for detecting a single index, the antibody chip detection kit for detecting the several detection indicators has the characteristics ofhigh specificity and high sensitivity, and the antibody chip also has the performance of small sample using amount. The invention also discloses detection methods using the antibody chip, and the detection methods comprise a fluorescence detection method based on sample labeling, a double-anti-sandwich method, and a detection method using the liquid suspension chip.

The invention discloses a method for determining serum alpha-amylase activity based on a paper base. The method comprises: a, paper-based alpha-amylase detection card preparation: 1.1, material preparation and 1.2, production: (1) preparing a dyeing starch solution, (2) spraying the dyeing starch solution on one side of a white filter paper to complete the production of the paper-based alpha-amylase detection film, (3) preparing a square film-fixing card consisting of upper and lower layers and (4) completing the preparation of the paper-based alpha-amylase detection card, and b, reaction. Themethod has low sample consumption (20 microliters of liquid), a fast response rate, high sensitivity, a wide linear range and a small system size, is easy to carry, is free of external reagents, improves the stability, reduces the cost of measurement, and provides a simple and low-cost diagnosis method of pancreatic diseases for primary medical units and people in special environmental areas.

The invention discloses an antibody chip kit for screening biomarkers. The kit comprises an antibody chip, a biotin labeled cell factor detection antibody mixture and horse radish peroxidase labeled streptavidin, wherein the antibody chip comprises a bas membrane and specific antibodies of 60 cell factors fixed on the surface of the base membrane, three positive control. The kit disclosed by the invention can be used for simultaneously detecting 60 cell factors for multiple samples, overcomes the defects in the prior art of tedious operation, single detection index, low sensitivity and the like and has the advantages that the kit is cheap, convenient, sensitive, accurate, high in throughput and few in use level of specimens and can be popularized and expanded on a common laboratory and the like.

The invention belongs to the technical field of biomedicine, and particularly relates to a gastric cancer detection kit adopting HOXB9 and PBX1 as biomarkers and an application. With the adoption of the detection kit, HOXB9 and PBX1 in a tissue sample are detected jointly, the gastric cancer is diagnosed and detected, and detection results have good sensitivity and high specificity. The kit can be effectively used for early screening and/or prognosis evaluation of the gastric cancer and has good clinical application prospect.

The invention relates to a leukemia marker joint inspection antibody chip and a kit. Leukemia markers are at least seventeen markers selected from ENA-78, GCP-2, MDC, MIF, MIP-3a, MPIE-1, MSPa, OPN, GDF-15, HB-EGF, HGF, PDGF-AA, VEGF-D, BLC, G-CSF, TNF-RI, uPAR, TNFa, Fas L, IL4, ICAM-1, IL5, KC, TIMP-1, TARC, IL2, IL7, M-CSF, IL10, IL21, IL-12p70, BDNF, PF-4, EGF, TNFRII and LIGHT. The expressionamounts of a plurality of samples and the leukemia markers can be measured at the same time, the leukemia occurrence and disease course are accurately and comprehensively reflected, and the leukemiadiagnosis, disease course monitoring and prognosis evaluation level is improved.

The invention relates to the technical field of reproductive medicine and immunological application and particularly relates to an antigen for detecting a sperm antibody and its expression vector, kitand detection method. The antigen for detecting a sperm antibody is a polypeptide encoded by a PB functional region gene fragment of a recombinant IZUMO gene and has a sequence shown in the formula of SEQ ID NO: 1. The antigen for detecting a sperm antibody is a key protein of a sperm acrosome reaction and determines if the fertilization is successful. The antibody for the antigen is an importantreason leading to immune infertility. The ELISA kit for detecting the anti-sperm antibody solves the problems of low sensitivity and low specificity, has the advantages of convenience, sensitivity, few sample use amount, large-scale detection and promotion and large-scale application in the general laboratories. Therefore, the antigen is expected to be used for screening and diagnosis of anti-sperm antibodies in infertile patients.