Method for determining serum alpha-amylase activity based on paper base

An amylase and paper-based technology, which is used in the fields of food, medicine, and biochemical testing, can solve problems that are not suitable for technicians in basic sanitation facilities, and achieve the effects of reducing measurement costs, wide linear range, and low cost

Inactive Publication Date: 2018-10-12
长春比特医疗仪器有限公司
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

Diagnostic assays currently available in developed countries require advanced laboratory infrastructure and expensive reagents, t

Method used

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  • Method for determining serum alpha-amylase activity based on paper base
  • Method for determining serum alpha-amylase activity based on paper base
  • Method for determining serum alpha-amylase activity based on paper base

Examples

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Embodiment 1

[0044] The present invention will be further described below in conjunction with accompanying drawing:

[0045] As shown in the figure, 1 is white filter paper, 1-1 is red dyed starch film, 1-2 is the natural color of white filter paper, 2 is paper-based α-amylase detection membrane, 2-1 is paper-based α-amylase Detecting the red film surface of the membrane, 3 is the diaphragm fixing card, 3-1 is the upper layer of the diaphragm fixing card, 3-1-1 is the upper circular opening of the diaphragm fixing card, 3-2 is the lower layer of the diaphragm fixing card, 4 is Paper-based alpha-amylase test card. 1. Sample

[0046]The research protocol was reviewed and approved by the Medical Ethics Committee of PLA 208 Hospital, and written informed consent was obtained. During the routine blood draw period (from March to August 2017), serum samples were collected from outpatients and inpatients in PLA 208 Hospital. Store in a 1.5ml eppendorf tube at 4°C and use within one week.

[004...

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Abstract

The invention discloses a method for determining serum alpha-amylase activity based on a paper base. The method comprises: a, paper-based alpha-amylase detection card preparation: 1.1, material preparation and 1.2, production: (1) preparing a dyeing starch solution, (2) spraying the dyeing starch solution on one side of a white filter paper to complete the production of the paper-based alpha-amylase detection film, (3) preparing a square film-fixing card consisting of upper and lower layers and (4) completing the preparation of the paper-based alpha-amylase detection card, and b, reaction. Themethod has low sample consumption (20 microliters of liquid), a fast response rate, high sensitivity, a wide linear range and a small system size, is easy to carry, is free of external reagents, improves the stability, reduces the cost of measurement, and provides a simple and low-cost diagnosis method of pancreatic diseases for primary medical units and people in special environmental areas.

Description

technical field [0001] The invention belongs to the technical fields of medicine, food and biochemical detection. Background technique [0002] Acute pancreatitis (AP) is one of the most common inflammatory diseases of the gastrointestinal tract, and its incidence has increased rapidly in recent years. Although the clinical course of most patients with acute pancreatitis is favorable, approximately 10%-20% of patients with severe acute pancreatitis develop a mortality rate of at least 30%. [0003] Serum α-amylase (Serum α-amylase, EC 3.2.1.1) is a kind of amylase, its function is to hydrolyze the glycosidic bond of starch polymer glucose and decompose polysaccharide. The human body is mainly synthesized and secreted by salivary glands and pancreatic epithelial cells, and also secreted by organs such as the lung, uterus, lactating breast, and proximal duodenum; in healthy people, serum amylase is mainly from the pancreas and salivary glands, of which 40-45% comes from Panc...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 侯巍吴再辉史健松石鑫
Owner 长春比特医疗仪器有限公司
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